Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,179	A→G	N429S (AAC→AGC)	cstA →	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,179	0	A	G	100.0%	265.7 / NA	69	N429S (AAC→AGC)	cstA	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/32);  total (37/32)

GTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTT  >  NC_000913/631031‑631326                                                                                                                                                     |                                                                                                                                                    gTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGcc                                                                                                                                                   >  2:162425/1‑151 (MQ=255)   ttCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGcc                                                                                                                                                   >  1:694405/1‑149 (MQ=255)     ccGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGcc                                                                                                                                                   >  1:649965/1‑147 (MQ=255)      cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGAtt                                                                                                                                              <  1:559466/151‑1 (MQ=255)       gCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTg                                                                                                                                             >  2:810482/1‑151 (MQ=255)             atcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCg                                                                                                                                       <  1:124995/151‑1 (MQ=255)                atcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGa                                                                                                                                      >  1:743685/1‑149 (MQ=255)                  cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAg                                                                                                                                    <  1:848996/149‑1 (MQ=255)                   gATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTgg                                                                                                                                 >  1:1059364/1‑151 (MQ=255)                     tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTggg                                                                                                                                >  1:641537/1‑150 (MQ=255)                        gggCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGaa                                                                                                                            <  1:1001335/151‑1 (MQ=255)                             tgtATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTc                                                                                                                       >  1:229647/1‑151 (MQ=255)                                aTTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGAt                                                                                                                    >  1:636201/1‑151 (MQ=255)                                 ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATc                                                                                                                   >  2:211407/1‑151 (MQ=255)                                        aTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTccc                                                                                                            >  1:765010/1‑151 (MQ=255)                                           aaCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTg                                                                                                         >  2:218813/1‑151 (MQ=255)                                           aaCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTg                                                                                                         <  2:271983/151‑1 (MQ=255)                                           aaCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTg                                                                                                         <  2:951816/151‑1 (MQ=255)                                            aCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTgc                                                                                                        <  1:227071/151‑1 (MQ=255)                                              aGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTgcgg                                                                                                      >  1:342506/1‑151 (MQ=255)                                               gCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTgcggg                                                                                                     >  1:969069/1‑151 (MQ=255)                                                 ccGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTgcgggcg                                                                                                   <  2:1073705/151‑1 (MQ=255)                                                   gATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGt                                                                                                 <  2:922467/151‑1 (MQ=255)                                                         ggTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCg                                                                                           >  1:717335/1‑151 (MQ=255)                                                            gCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGAc                                                                                         <  1:918069/150‑1 (MQ=255)                                                                  tCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCg                                                                                  >  1:456078/1‑151 (MQ=255)                                                                    ctgCAGTGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGGAGCTGGGGCTTTAGCATTACACCAGATGTCCTAAGCCAGATTGCCAGCGAAGTGGGTGAGCAGTCGGTCATTTCCCGTGCGCGCGGTGCGCCGACGCTGGCgg                                                                                 <  1:1042433/148‑1 (MQ=255)                                                                     ggCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTg                                                                               <  1:1047902/151‑1 (MQ=255)                                                                      gCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTgg                                                                              >  2:522249/1‑151 (MQ=255)                                                                            aCGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATgg                                                                        <  2:136842/151‑1 (MQ=255)                                                                             cggcggATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGc                                                                       >  1:354360/1‑151 (MQ=255)                                                                                  gATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACa                                                                  <  1:1068873/151‑1 (MQ=255)                                                                                      tgGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCt                                                              >  1:827330/1‑151 (MQ=255)                                                                                       ggTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCt                                                              <  1:1045519/150‑1 (MQ=255)                                                                                        gTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGc                                                            >  2:470023/1‑151 (MQ=255)                                                                                         tCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGc                                                            >  2:100101/1‑150 (MQ=255)                                                                                          cGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCa                                                           <  2:301878/150‑1 (MQ=255)                                                                                                  ccGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCt                                                  <  2:707466/151‑1 (MQ=255)                                                                                                      gcAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGggc                                              <  1:922405/151‑1 (MQ=255)                                                                                                       cAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGggcg                                             >  1:65158/1‑151 (MQ=255)                                                                                                               gAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCatgatg                                     <  2:1017579/151‑1 (MQ=255)                                                                                                                 gTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatgga                                   <  1:323004/151‑1 (MQ=255)                                                                                                                   aGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggatg                                 <  1:44303/151‑1 (MQ=255)                                                                                                                   aGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggat                                  >  1:614705/1‑150 (MQ=255)                                                                                                                   aGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggat                                  <  2:794818/150‑1 (MQ=255)                                                                                                                   aGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggat                                  <  1:382466/150‑1 (MQ=255)                                                                                                                       ggggCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTgg                              >  1:1160215/1‑150 (MQ=255)                                                                                                                       ggggCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTgg                              >  2:171350/1‑150 (MQ=255)                                                                                                                       ggggCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGc                             <  2:863723/151‑1 (MQ=255)                                                                                                                           cTTTAGAATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGtt                          >  1:496521/1‑150 (MQ=255)                                                                                                                            tttAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCt                        >  1:527054/1‑151 (MQ=255)                                                                                                                             ttAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCt                        <  2:1093461/150‑1 (MQ=255)                                                                                                                             ttAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTg                       >  2:470606/1‑151 (MQ=255)                                                                                                                             ttAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTg                       >  1:437925/1‑151 (MQ=255)                                                                                                                             ttAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCACGATGGATGTGGCGGTCt                        >  1:1046040/1‑150 (MQ=255)                                                                                                                                gCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGt                     >  2:298621/1‑150 (MQ=255)                                                                                                                                gCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTa                    <  2:379386/151‑1 (MQ=255)                                                                                                                                 cATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTAt                   >  2:502165/1‑151 (MQ=255)                                                                                                                                   ttACACCAGATACGCTAAGCCAGATTGCCAGCGACGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGCGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTAt                   >  1:438545/1‑149 (MQ=255)                                                                                                                                   ttACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATc                  >  1:38143/1‑150 (MQ=255)                                                                                                                                       acCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTc             >  2:1155921/1‑151 (MQ=255)                                                                                                                                            aTACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAt        <  1:505823/151‑1 (MQ=255)                                                                                                                                              aCGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAttt      <  2:1117016/151‑1 (MQ=255)                                                                                                                                              aCGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAtt       <  1:775987/150‑1 (MQ=255)                                                                                                                                              aCGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAtt       <  2:948821/150‑1 (MQ=255)                                                                                                                                               cGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAttt      <  1:413070/150‑1 (MQ=255)                                                                                                                                                gCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg    <  2:1045979/151‑1 (MQ=255)                                                                                                                                                gCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttg    >  2:798647/1‑151 (MQ=255)                                                                                                                                                  tAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgtt  >  2:492657/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTT  >  NC_000913/631031‑631326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑