Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,563	A→G	S210P (TCT→CCT)	rlpA ←	septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,563	0	A	G	100.0%	301.9 / NA	80	S210P (TCT→CCT)	rlpA	septal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/37);  total (43/37)

GCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGT  >  NC_000913/664415‑664709                                                                                                                                                     |                                                                                                                                                   gcAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGgca                                                                                                                                                  <  2:434437/151‑1 (MQ=255)  cAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGgcac                                                                                                                                                 >  1:1025435/1‑151 (MQ=255)     gCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTg                                                                                                                                               >  1:1061982/1‑150 (MQ=255)       gTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAAc                                                                                                                                            <  2:961850/151‑1 (MQ=255)         cGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACtt                                                                                                                                          >  2:266989/1‑151 (MQ=255)         cGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACtt                                                                                                                                          >  1:1170603/1‑151 (MQ=255)               gCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTccc                                                                                                                                    >  1:224883/1‑151 (MQ=255)                cTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCcg                                                                                                                                   >  1:67969/1‑151 (MQ=255)                       ccAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCAcc                                                                                                                            <  2:517158/151‑1 (MQ=255)                       ccAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCAc                                                                                                                             <  2:1018874/150‑1 (MQ=255)                           tACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCtt                                                                                                                        >  1:213996/1‑151 (MQ=255)                                   gcgcTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCggg                                                                                                                <  2:1125227/151‑1 (MQ=255)                                         aGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgc                                                                                                          <  1:443627/151‑1 (MQ=255)                                          ggTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgca                                                                                                         <  1:524185/151‑1 (MQ=255)                                             cGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgcaggc                                                                                                      >  1:1097644/1‑151 (MQ=255)                                             cGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgcaggc                                                                                                      >  1:571960/1‑151 (MQ=255)                                               ttGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgcaggc                                                                                                      >  1:374109/1‑149 (MQ=255)                                                tGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTgcaggcag                                                                                                    <  2:620420/150‑1 (MQ=255)                                                    gcgcCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCg                                                                                                <  1:106605/150‑1 (MQ=255)                                                      gcCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTa                                                                                              >  2:1187390/1‑150 (MQ=255)                                                         gAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTc                                                                                          >  1:738762/1‑151 (MQ=255)                                                          aGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCt                                                                                         <  2:955320/151‑1 (MQ=255)                                                          aGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCt                                                                                         <  2:312533/151‑1 (MQ=255)                                                              aaCCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGttt                                                                                     <  1:1152292/151‑1 (MQ=255)                                                                 cGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGc                                                                                  <  2:1165003/151‑1 (MQ=255)                                                                  gCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCg                                                                                 <  1:1156934/151‑1 (MQ=255)                                                                   cTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCg                                                                                 >  1:211334/1‑150 (MQ=255)                                                                     aCTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGAc                                                                               <  1:878992/150‑1 (MQ=255)                                                                     aCTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACt                                                                              <  2:1003535/151‑1 (MQ=255)                                                                       tGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACtg                                                                             >  2:287330/1‑150 (MQ=255)                                                                         gTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTgg                                                                          >  2:349315/1‑151 (MQ=255)                                                                           tACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGt                                                                         >  2:102942/1‑150 (MQ=255)                                                                              cGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACaa                                                                     <  1:1068494/151‑1 (MQ=255)                                                                                gcgcgcCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGc                                                                   <  2:1084534/151‑1 (MQ=255)                                                                                    gcCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCAt                                                                >  1:563114/1‑150 (MQ=255)                                                                                    gcCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCAt                                                                <  2:854949/150‑1 (MQ=255)                                                                                        gTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAg                                                           <  2:398529/151‑1 (MQ=255)                                                                                         tCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAgg                                                          >  1:429266/1‑151 (MQ=255)                                                                                           ggctCTTCGCTTTATAGCGTCGAATTACTGCCCGGAAGAATGTCACCCGGCGGGCCAGGCGCTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGGGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGa                                                         <  1:38960/147‑1 (MQ=255)                                                                                           ggATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGAc                                                        >  1:165448/1‑151 (MQ=255)                                                                                           ggATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGAc                                                        >  1:260816/1‑151 (MQ=255)                                                                                           ggATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGAc                                                        <  2:1056332/151‑1 (MQ=255)                                                                                                 tCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCCCCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAg                                                     <  1:423305/148‑1 (MQ=255)                                                                                                  cGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGaaa                                                  <  2:755589/150‑1 (MQ=255)                                                                                                  cGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGaa                                                   >  1:743291/1‑149 (MQ=255)                                                                                                   gCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGc                                                >  1:3423/1‑151 (MQ=255)                                                                                                    cTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCg                                               >  2:908595/1‑151 (MQ=255)                                                                                                    cTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCg                                               >  1:98129/1‑151 (MQ=255)                                                                                                     tttttAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCg                                               >  2:1022293/1‑150 (MQ=255)                                                                                                      ttttAGCGTCGAATTCCTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGTTTAAATCGGGAGGTGCAGGCGGGGCGTAAGTCTGTTTGGCGACTGGGGTACAAGCCATACCAGGACCAGAAAGc                                                <  1:367560/148‑1 (MQ=255)                                                                                                      ttttAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGa                                              <  2:360775/150‑1 (MQ=255)                                                                                                       tttAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGaa                                             <  2:327599/150‑1 (MQ=255)                                                                                                        ttAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAAcc                                           >  1:260049/1‑151 (MQ=255)                                                                                                         tAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAAcc                                           <  2:461331/150‑1 (MQ=255)                                                                                                           gCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCAt                                         >  2:188618/1‑150 (MQ=255)                                                                                                           gCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATc                                        >  2:474223/1‑151 (MQ=255)                                                                                                            cGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATcc                                       >  1:734121/1‑151 (MQ=255)                                                                                                             ggcGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATGGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATcc                                       <  2:135068/148‑1 (MQ=255)                                                                                                             gTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCt                                      >  1:544299/1‑151 (MQ=255)                                                                                                             gTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCt                                      <  1:1108514/151‑1 (MQ=255)                                                                                                              tCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGAGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTg                                     >  1:1086883/1‑151 (MQ=255)                                                                                                               cGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTgg                                    >  2:577295/1‑151 (MQ=255)                                                                                                                 aaTTACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGc                                  >  1:512878/1‑151 (MQ=255)                                                                                                                    tACTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGcaa                                <  2:325730/150‑1 (MQ=255)                                                                                                                     aCTGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGcaac                               <  1:1110682/150‑1 (MQ=255)                                                                                                                       tGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCAt                                         >  2:542029/1‑138 (MQ=255)                                                                                                                       tGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCAt                                         <  1:542026/138‑1 (MQ=255)                                                                                                                       tGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGcaa                                <  2:429101/147‑1 (MQ=255)                                                                                                                       tGACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGcaa                                >  1:429098/1‑147 (MQ=255)                                                                                                                        gACCGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACaat                            <  2:234710/150‑1 (MQ=255)                                                                                                                          ccGGAAGAATGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACaataa                          <  2:279279/150‑1 (MQ=255)                                                                                                                                gaaTGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGAt                    <  1:437688/150‑1 (MQ=255)                                                                                                                                gaaTGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATc                   >  2:1048291/1‑151 (MQ=255)                                                                                                                                gaaTGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATc                   >  1:493677/1‑151 (MQ=255)                                                                                                                                 aaTGTCACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATc                   <  1:405825/150‑1 (MQ=255)                                                                                                                                      cACCCTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACg             >  1:192439/1‑151 (MQ=255)                                                                                                                                        cccTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGa            >  1:124117/1‑150 (MQ=255)                                                                                                                                         ccTGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAAc          >  2:225782/1‑151 (MQ=255)                                                                                                                                           tGCGGGCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACtt        >  2:299768/1‑151 (MQ=255)                                                                                                                                              gggCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTgg     >  2:1080107/1‑151 (MQ=255)                                                                                                                                               ggCCAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGgt    >  1:155823/1‑151 (MQ=255)                                                                                                                                                 ccAGGCACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGgtgt  >  2:177051/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCAGGCGTCGGTTCGCTGCCTTCCAGTACACCAGGCGCTAAGGTCGTTGGTGCGCCGAGGAAACCGCTACTGGTTACCGGCGCGCCGGTCGGATCTTCGCTTTTTAGCGTCGAATTACTGACCGGAAGAATGTCACCCTGCGGGCCAGACACTGAACTTGTTCCCGCGCCACCGCTTAAATCGGGAGGTGCAGGCAGGGCGTAAGTCTGTTTGGCGACTGTGGTACAAGCCATACCAGGACCAGAAAGCGAACCATCCTGGGCAACAATAATCGGATCGATACGAACTTTGGTGT  >  NC_000913/664415‑664709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑