Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	999,721	A→G	D169G (GAT→GGT)	elfC →	putative outer membrane fimbrial subunit export usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	999,721	0	A	G	100.0%	247.3 / NA	66	D169G (GAT→GGT)	elfC	putative outer membrane fimbrial subunit export usher protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

AAACAAGCGTGTGTGCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGATGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGGAGATTGCGCAAC  >  NC_000913/999579‑999863                                                                                                                                               |                                                                                                                                               aaacaaGCGTGTGTGCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGAGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGa                                                                                                                                        <  1:764171/151‑1 (MQ=255)      aGCGTGTGTGCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAAc                                                                                                                                   <  1:886584/151‑1 (MQ=255)        cGTGTGTGCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAAcgc                                                                                                                                 <  1:960297/151‑1 (MQ=255)            tgtgCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCt                                                                                                                              >  1:796300/1‑150 (MQ=255)             gtgCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTa                                                                                                                             <  2:741547/150‑1 (MQ=255)             gtgCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTAt                                                                                                                            <  2:903544/151‑1 (MQ=255)               gCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTa                                                                                                                          <  1:1164016/151‑1 (MQ=255)                      gCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGat                                                                                                                   <  1:23011/151‑1 (MQ=255)                       cGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGata                                                                                                                  <  1:383944/151‑1 (MQ=255)                        ggAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGatat                                                                                                                 >  2:814557/1‑151 (MQ=255)                               attCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGttt                                                                                                           <  2:1172027/150‑1 (MQ=255)                                  ccGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCg                                                                                                       >  1:827427/1‑151 (MQ=255)                                   cGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTTCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCgg                                                                                                      >  2:977007/1‑151 (MQ=255)                                    ggATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCggg                                                                                                     >  2:252482/1‑151 (MQ=255)                                    ggATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCggg                                                                                                     >  2:392346/1‑151 (MQ=255)                                      aTGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGc                                                                                                   <  2:548306/151‑1 (MQ=255)                                        gCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCt                                                                                                  <  2:637995/150‑1 (MQ=255)                                            gCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAg                                                                                             <  2:505351/151‑1 (MQ=255)                                             cGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGt                                                                                            >  1:910712/1‑151 (MQ=255)                                              gTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGt                                                                                            >  1:311323/1‑150 (MQ=255)                                                aaCGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTAtt                                                                                         <  2:741222/151‑1 (MQ=255)                                                 aCGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTTAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTc                                                                                        <  1:799429/151‑1 (MQ=255)                                                 aCGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTc                                                                                        <  2:90035/151‑1 (MQ=255)                                                 aCGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTc                                                                                        <  1:1093808/151‑1 (MQ=255)                                                  cGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTc                                                                                        >  1:533076/1‑150 (MQ=255)                                                  cGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCa                                                                                       <  1:1093389/151‑1 (MQ=255)                                                   gTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCAt                                                                                      >  1:322040/1‑151 (MQ=255)                                                          gtgAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAgcg                                                                               <  1:679683/151‑1 (MQ=255)                                                              aTAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAgcgc                                                                              >  1:579470/1‑148 (MQ=255)                                                               tAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGAc                                                                          >  1:816389/1‑151 (MQ=255)                                                                 aaCTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGAc                                                                          >  2:285549/1‑149 (MQ=255)                                                                 aaCTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAg                                                                        <  2:553664/151‑1 (MQ=255)                                                                     ccGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTg                                                                      <  2:552761/149‑1 (MQ=255)                                                                          tGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTgg                                                               >  1:103941/1‑151 (MQ=255)                                                                           ggAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGCCAGTGATTCTgg                                                               <  1:1061229/150‑1 (MQ=255)                                                                           ggAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCt                                                                 <  1:153916/148‑1 (MQ=255)                                                                           ggAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTgg                                                               <  1:43319/150‑1 (MQ=255)                                                                            gAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTgg                                                               >  2:794123/1‑149 (MQ=255)                                                                            gAAATTTCAAGTCCGCAAAACGGCATCAAAAGTAAAGCCCGTGGGTATTTCCCCCCTGAACGTTGGGGTGAAAGGATCAACGCGCTATTACTTGGGTATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTg                                                                <  2:673342/148‑1 (MQ=255)                                                                             aaaTTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCg                                                             >  2:247824/1‑150 (MQ=255)                                                                             aaaTTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGa                                                            <  2:928919/151‑1 (MQ=255)                                                                                   cAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCt                                                       <  2:7868/150‑1 (MQ=255)                                                                                   cAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCt                                                       <  2:194356/150‑1 (MQ=255)                                                                                    aGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTAt                                                     >  2:601536/1‑151 (MQ=255)                                                                                    aGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTAt                                                     <  1:759273/151‑1 (MQ=255)                                                                                       aCCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTAttt                                                   <  1:392343/150‑1 (MQ=255)                                                                                             aaTCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAAt                                            >  2:173050/1‑151 (MQ=255)                                                                                              aTCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAAtt                                           <  2:461738/151‑1 (MQ=255)                                                                                                  ccATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTaa                                        >  2:72714/1‑150 (MQ=255)                                                                                                   cATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAAc                                      <  1:476133/151‑1 (MQ=255)                                                                                                     tCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAAc                                      >  2:877319/1‑149 (MQ=255)                                                                                                     tCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAAc                                      >  2:595636/1‑149 (MQ=255)                                                                                                      cAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAg                                    <  2:543998/150‑1 (MQ=255)                                                                                                         aaGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGc                                >  2:1150866/1‑151 (MQ=255)                                                                                                          aGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCg                               >  2:1124463/1‑151 (MQ=255)                                                                                                             aaTGCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGtt                             >  2:1057879/1‑150 (MQ=255)                                                                                                                gCCCGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAAtt                         <  2:907432/151‑1 (MQ=255)                                                                                                                 cccGTGGTTATGTCCCCCCTGAACGTTGGGGCGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAAttt                        >  2:1124701/1‑151 (MQ=255)                                                                                                                  ccGTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAAttt                        >  1:601368/1‑150 (MQ=255)                                                                                                                    gTGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAgg                     >  1:324537/1‑151 (MQ=255)                                                                                                                     tGGTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGATATTGTCTGAATTTAAACAGTGGCGTTAATTTAgg                     <  2:419661/150‑1 (MQ=255)                                                                                                                      ggTTATGTCCCCCCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGcc                   <  1:1063486/151‑1 (MQ=255)                                                                                                                             tCCCACCTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGTAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGga             <  1:597676/150‑1 (MQ=255)                                                                                                                                ccccTGAACGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGGAGAtt         >  2:549984/1‑151 (MQ=255)                                                                                                                                      aaCGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGGAGATTGCGca    >  2:876840/1‑150 (MQ=255)                                                                                                                                       aCGTTGGGGTGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGGAGATTGCGcaac  >  2:983753/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                               AAACAAGCGTGTGTGCCATTGGCGGAGATTATTCCGGATGCCAGCGTAACGTTTAATGTGAATAAACTCCGTCTGGAAATTTCAGTACCGCAAATCGCCATCAAAAGTAATGCCCGTGGTTATGTCCCCCCTGAACGTTGGGATGAAGGGATCAACGCGCTATTACTGGGATATTCGTTTAGCGGGGCTAACAGTATTCATAGCAGCGCAGACAGTGATTCTGGCGACAGCTATTTTCTGAATTTAAACAGTGGCGTTAATTTAGGCCCATGGAGATTGCGCAAC  >  NC_000913/999579‑999863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑