Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,682	A→G	T424A (ACA→GCA)	ftsI →	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,682	0	A	G	100.0%	325.0 / NA	84	T424A (ACA→GCA)	ftsI	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/44);  total (40/44)

GTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAA  >  NC_000913/92537‑92829                                                                                                                                                  |                                                                                                                                                    gTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCAcc                                                                                                                                                 >  1:347400/1‑150 (MQ=255)   tattagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCAtt                                                                                                                                              >  1:164073/4‑151 (MQ=255)    agtagATACTTACTCACGCTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTa                                                                                                                                             <  1:455441/151‑1 (MQ=255)      tagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTAc                                                                                                                                            >  1:899205/1‑150 (MQ=255)       agATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTAc                                                                                                                                            >  1:1015754/1‑149 (MQ=255)       agATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACa                                                                                                                                           <  2:899091/150‑1 (MQ=255)       agATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACa                                                                                                                                           <  2:601847/150‑1 (MQ=255)       agATACGTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACa                                                                                                                                           <  1:49432/150‑1 (MQ=255)         aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                        <  2:122476/151‑1 (MQ=255)         aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                        <  2:1176641/151‑1 (MQ=255)         aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                        <  1:750255/151‑1 (MQ=255)         aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                        >  1:350351/1‑151 (MQ=255)           acttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                        >  1:248805/1‑149 (MQ=255)            cttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAgc                                                                                                                                     >  1:874870/1‑151 (MQ=255)            cttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAgc                                                                                                                                     >  2:408215/1‑151 (MQ=255)              tactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAgcgc                                                                                                                                   >  1:789935/1‑151 (MQ=255)               actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGcgcg                                                                                                                                  >  2:67955/1‑151 (MQ=255)                    cGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGa                                                                                                                                 <  1:1097530/147‑1 (MQ=255)                    cGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCt                                                                                                                             <  1:14721/151‑1 (MQ=255)                      ttttGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTAc                                                                                                                           >  2:814180/1‑151 (MQ=255)                        ttGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGc                                                                                                                         >  1:950123/1‑151 (MQ=255)                        ttGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGc                                                                                                                         >  2:1074607/1‑151 (MQ=255)                          ggACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCa                                                                                                                        <  2:99670/150‑1 (MQ=255)                               gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTAt                                                                                                                   <  2:675445/150‑1 (MQ=255)                               gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTAt                                                                                                                   <  1:509950/150‑1 (MQ=255)                               gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATc                                                                                                                  <  1:827297/151‑1 (MQ=255)                                 gAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCgg                                                                                                                <  1:123695/151‑1 (MQ=255)                                  aaaaGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGc                                                                                                               >  2:566569/1‑151 (MQ=255)                                    aaGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAg                                                                                                             <  1:808098/151‑1 (MQ=255)                                        gACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTAc                                                                                                         >  2:21743/1‑151 (MQ=255)                                          ccAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACgg                                                                                                       >  1:57434/1‑151 (MQ=255)                                                  gggTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATc                                                                                               <  2:516318/151‑1 (MQ=255)                                                      tGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGcc                                                                                            <  1:819702/150‑1 (MQ=255)                                                       ggTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGccc                                                                                           >  1:11239/1‑150 (MQ=255)                                                           ggAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGt                                                                                      >  2:392510/1‑151 (MQ=255)                                                            gagaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTc                                                                                     <  2:907066/151‑1 (MQ=255)                                                            gagaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTc                                                                                     <  1:439812/151‑1 (MQ=255)                                                            gagaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCCCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTc                                                                                     >  2:578401/1‑151 (MQ=255)                                                              gaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCg                                                                                    <  1:276756/150‑1 (MQ=255)                                                               aaCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGAt                                                                                  <  2:805340/151‑1 (MQ=255)                                                                aCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGAtt                                                                                 <  1:420134/151‑1 (MQ=255)                                                                 cGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTa                                                                                <  2:27634/151‑1 (MQ=255)                                                                  gCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTAc                                                                               >  2:452701/1‑151 (MQ=255)                                                                   cAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTAcc                                                                              >  1:625217/1‑151 (MQ=255)                                                                     gTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCaa                                                                            <  1:242210/151‑1 (MQ=255)                                                                     gTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCa                                                                             >  2:350807/1‑150 (MQ=255)                                                                       ggCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCaaa                                                                           <  2:783207/150‑1 (MQ=255)                                                                       ggCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCaaa                                                                           <  2:11239/150‑1 (MQ=255)                                                                         cTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGtt                                                                        >  1:34369/1‑151 (MQ=255)                                                                          ttATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCCTTTATCGCCCACTGTCGATTACCAAAGTTg                                                                       >  1:68559/1‑151 (MQ=255)                                                                              atCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAccc                                                                   >  1:25873/1‑151 (MQ=255)                                                                                ccTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccc                                                                  <  2:268180/150‑1 (MQ=255)                                                                                  tCAAAAACAACGGTGGGCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCg                                                               >  2:82912/1‑151 (MQ=255)                                                                                   cAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccccc                                                                <  1:122679/149‑1 (MQ=255)                                                                                     aaaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACATTTATCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGtt                                                            <  1:579906/151‑1 (MQ=255)                                                                                        acaacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTccc                                                         >  1:773373/1‑151 (MQ=255)                                                                                         caacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCg                                                        <  2:53944/151‑1 (MQ=255)                                                                                          aacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCgg                                                       >  2:549512/1‑151 (MQ=255)                                                                                          aacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCgg                                                       <  1:145585/151‑1 (MQ=255)                                                                                            cGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGt                                                      <  2:834101/150‑1 (MQ=255)                                                                                                 gtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgt                                                >  1:1198284/1‑151 (MQ=255)                                                                                                     gACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtgt                                              <  1:1001365/149‑1 (MQ=255)                                                                                                     gACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtg                                               <  2:498919/148‑1 (MQ=255)                                                                                                     gACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtg                                               <  1:659010/148‑1 (MQ=255)                                                                                                       cATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTc                                          >  2:1004424/1‑151 (MQ=255)                                                                                                           gagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCgg                                      <  2:950143/151‑1 (MQ=255)                                                                                                             gagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtgt                                              <  1:66488/141‑1 (MQ=255)                                                                                                             gagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtgt                                              >  2:66488/1‑141 (MQ=255)                                                                                                                   cACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCAtt                              <  1:801453/151‑1 (MQ=255)                                                                                                                    aCCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTg                             <  2:641609/151‑1 (MQ=255)                                                                                                                        tctcTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTcc                          <  2:361684/150‑1 (MQ=255)                                                                                                                        tctcTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTc                           <  1:908857/149‑1 (MQ=255)                                                                                                                        tctcTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCg                         >  1:525254/1‑151 (MQ=255)                                                                                                                         ctctTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCGGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGc                        >  1:325705/1‑151 (MQ=255)                                                                                                                             ttCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACtg                    >  1:34300/1‑151 (MQ=255)                                                                                                                                ggCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTgtgg                 >  2:1087440/1‑151 (MQ=255)                                                                                                                                ggCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTgtgg                 <  1:177379/151‑1 (MQ=255)                                                                                                                                    aCGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTgtggtg               <  2:1040714/149‑1 (MQ=255)                                                                                                                                         cTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatg         >  2:737200/1‑150 (MQ=255)                                                                                                                                          tAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatgat       >  1:760813/1‑151 (MQ=255)                                                                                                                                           aaTGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatgatg      >  2:600713/1‑151 (MQ=255)                                                                                                                                           aaTGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatgatg      >  1:101629/1‑151 (MQ=255)                                                                                                                                             tGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATgg     >  1:798940/1‑150 (MQ=255)                                                                                                                                               gTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGaaa  <  2:761096/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAA  >  NC_000913/92537‑92829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑