| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,450 | T→C | intergenic (+621/+115) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,450 | 0 | T | C | 83.3% | 22.2 / ‑0.2 | 27 | intergenic (+621/+115) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (8/12); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
GTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC > NC_000913/1097305‑1097597 | gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCTTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTc < 1:799033/151‑1 (MQ=14) gggggggctgggccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCt < 1:381043/133‑1 (MQ=255) ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCCTCGCTCCCCCTGCGGGCAACCCCCTCCCTGCGTTCCCGGCCTGTcc > 2:786976/1‑147 (MQ=11) ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAGTGACTCGCCTCGCTCGCCCCCCGGGCAGCCCCCTCCCCGCGTTCACGGCCTGTccca > 2:420351/1‑147 (MQ=11) ggCTTCTCAT‑CCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAGTGAATCCCCTCGCTCCCCCCGCGGGCAACCCCCTCCCCGCGTTCACGGTCTTTCCCAcc > 1:563221/1‑149 (MQ=11) gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTg < 2:16246/151‑1 (MQ=14) ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCCCTTCGCCCTCCCTCGGGGGAGCCCTCTTAATGCGTTCGCGGCCGGGCCAAccg > 1:964869/1‑147 (MQ=11) aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCCCCTCCCCCGCCCCCCGGGCAACCCCCCCCCTTCGTTCCCGGCCCGTCCCACTgg > 2:1002250/1‑145 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:724423/150‑1 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:565142/150‑1 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGGTCTGTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:229339/150‑1 (MQ=2) tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAATCGCTTCGCTCGCCCCGCGGGGCGCCCCCTCCCTGCGTTTACCGGCTGTCCAACTGGCTTTCGCCCGTTTTC‑GAAcc > 2:838476/1‑151 (MQ=11) gCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCCCGCCCTGCGGGCAGCCCCCCCACCGCGTTCACGGGCTGTCCAACTGGCTGTCGCCCGGTGTC‑GAAccc > 2:1012801/1‑151 (MQ=11) gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCGCTTCCCCCGCCCTGCGGGCAGCCCCCTCCCTGCGGTCACGGCCTGTCCAACTCGCTGTCGCCCGTTGTC‑GAAccc > 1:296483/1‑151 (MQ=11) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAATCGCTTTGCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTCACGGCCTGTCCAACCGGCTGTCGCCAGTTGTC‑GAACCCCgg > 2:705862/1‑151 (MQ=11) taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCCCCTCCCTCGCCCCGCGGGGAGGCCGGTCGGTGGGGTGCCGGGCCGTCCCACTGGCTGTCGGCAGGTGTCGGAACCCCggc > 1:76993/1‑149 (MQ=2) cTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGaa < 1:705852/150‑1 (MQ=17) tGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAcc > 1:196762/1‑151 (MQ=14) tGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa < 2:551706/148‑1 (MQ=14) gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAcc < 2:491156/150‑1 (MQ=33) gCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTCggaacc > 1:1103129/1‑146 (MQ=9) cGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAcccc < 2:27504/151‑1 (MQ=25) gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAccccc < 1:415976/151‑1 (MQ=14) gTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAccccc > 2:649558/1‑151 (MQ=25) cACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGa < 2:348899/150‑1 (MQ=38) cGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATa < 2:76994/150‑1 (MQ=255) cGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAc < 2:1048029/151‑1 (MQ=255) | GTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC > NC_000913/1097305‑1097597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |