Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,262,619	C→T	G87R (GGA→AGA)	ispE ←	4‑diphosphocytidyl‑2‑C‑methylerythritol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,262,619	0	C	T	100.0%	245.8 / NA	61	G87R (GGA→AGA)	ispE	4‑diphosphocytidyl‑2‑C‑methylerythritol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/32);  total (29/32)

AGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCG  >  NC_000913/1262471‑1262764                                                                                                                                                     |                                                                                                                                                  aGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCg                                                                                                                                                 >  2:916647/1‑151 (MQ=255)   ccccATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCCCCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGt                                                                                                                                                <  2:211249/150‑1 (MQ=255)     ccATTCCCGCCACCTCACCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCgg                                                                                                                                             <  1:122771/151‑1 (MQ=255)      cATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCgg                                                                                                                                             >  1:340213/1‑150 (MQ=255)              ccAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGCCCGTCGCGGCTTTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGccc                                                                                                                                    <  1:300078/151‑1 (MQ=255)               cAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCg                                                                                                                                   >  2:196702/1‑151 (MQ=255)                     aTCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCt                                                                                                                             >  2:292873/1‑151 (MQ=255)                      tcaaTGCTGAGCCCGCATTGCCAGAGATGATTAAATGCCCCCAGGACCTTCGCGGAATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCt                                                                                                                             <  1:871168/147‑1 (MQ=255)                       ccATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGACCCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGc                                                                                                                           <  1:278580/151‑1 (MQ=255)                         aTGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCg                                                                                                                         >  2:979436/1‑151 (MQ=255)                             ttAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGCCCGCCGCGGCATTGGATGACCCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCa                                                                                                                       <  2:340216/149‑1 (MQ=255)                                 cccGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGACCCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGtttt                                                                                                                  <  2:682047/150‑1 (MQ=255)                                 cccGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtc                                                                                                                 >  1:120980/1‑151 (MQ=255)                                 cccGCATTGCCAGAGATGATTTAATCCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGtt                                                                                                                    <  1:919097/148‑1 (MQ=255)                                  ccGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtca                                                                                                                <  2:673514/151‑1 (MQ=255)                                     cATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtcatc                                                                                                              >  1:462488/1‑150 (MQ=255)                                     cATTGCCAGAGATGATTAAATGCCACCAGGCCCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTtcat                                                                                                               <  1:401755/149‑1 (MQ=255)                                          ccAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAAt                                                                                                        >  1:839444/1‑151 (MQ=255)                                          ccAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAAt                                                                                                        <  2:822618/151‑1 (MQ=255)                                          ccAGAGATGATTTAAGGCCCCCAGGCCCGTCGCGGCATTGGATGACCCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATcaacaa                                                                                                         <  1:616305/150‑1 (MQ=255)                                           cAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATc                                                                                                       >  2:1109380/1‑151 (MQ=255)                                           cAGAGATGATTTAATGCCACCAGGACCGTCGCGGCACTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATc                                                                                                       >  1:135832/1‑151 (MQ=255)                                            agagATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATc                                                                                                       >  1:390059/1‑150 (MQ=255)                                                     ttAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTgcgc                                                                                              <  2:264436/150‑1 (MQ=255)                                                     ttAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGcgcg                                                                                             >  2:1174048/1‑151 (MQ=255)                                                      tAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGcgcg                                                                                             >  1:473632/1‑150 (MQ=255)                                                       aaTGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGaa                                                                                           <  1:913025/151‑1 (MQ=255)                                                        aTGCCACCAGGACCGTCGCGGCATTGGCTGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAAc                                                                                          >  2:651209/1‑151 (MQ=255)                                                           ccaccaGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGAt                                                                                       >  2:950966/1‑151 (MQ=255)                                                           ccaccaGGACCGTCGCGGCATGGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACg                                                                                         <  1:1086144/149‑1 (MQ=255)                                                                     cGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCt                                                                             >  1:122418/1‑151 (MQ=255)                                                                        cgcgGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                                                                           >  1:906259/1‑150 (MQ=255)                                                                         gcgGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTc                                                                           <  1:821669/149‑1 (MQ=255)                                                                         gcgGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCAt                                                                         >  1:475674/1‑151 (MQ=255)                                                                            gcttgggATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATg                                                                        <  2:1099664/144‑1 (MQ=255)                                                                             cATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTc                                                                     <  2:868229/151‑1 (MQ=255)                                                                             cATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTc                                                                     <  2:459056/151‑1 (MQ=255)                                                                                tGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCa                                                                   <  2:985165/150‑1 (MQ=255)                                                                                 ggATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCAc                                                                  <  1:169196/150‑1 (MQ=255)                                                                                             gccgAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGc                                                     <  1:312347/151‑1 (MQ=255)                                                                                                      gccgccCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGa                                            <  1:346073/151‑1 (MQ=255)                                                                                                        cgccCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACg                                          >  1:1010065/1‑151 (MQ=255)                                                                                                             aTCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAatat                                     >  1:359032/1‑151 (MQ=255)                                                                                                             aTCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAatat                                     <  1:196700/151‑1 (MQ=255)                                                                                                                   aaaCGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCAt                               >  2:101751/1‑151 (MQ=255)                                                                                                                   aaaCGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCAt                               >  1:505987/1‑151 (MQ=255)                                                                                                                     aCGCTTGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCg                             <  1:362665/151‑1 (MQ=255)                                                                                                                          tGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCa                          <  2:710764/149‑1 (MQ=255)                                                                                                                          tGTCAATGCTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCa                          <  1:89087/149‑1 (MQ=255)                                                                                                                               aTGCTGATATTCGCACCGCTTCTCGTCGGAAGATGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCt                   <  1:244826/151‑1 (MQ=255)                                                                                                                                  cTGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCa                 >  1:209726/1‑150 (MQ=255)                                                                                                                                   tGATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAAt               >  1:1070476/1‑151 (MQ=255)                                                                                                                                    gATATTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAAt               <  1:554130/150‑1 (MQ=255)                                                                                                                                      tatTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCt            >  1:33535/1‑151 (MQ=255)                                                                                                                                       atTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCAc                         <  2:15661/137‑1 (MQ=255)                                                                                                                                       atTCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCAc                         >  1:15661/1‑137 (MQ=255)                                                                                                                                        ttCGCACCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTg           >  2:990784/1‑150 (MQ=255)                                                                                                                                             aCCGCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGgtg     <  1:160080/151‑1 (MQ=255)                                                                                                                                                gCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCg  >  2:420758/1‑151 (MQ=255)                                                                                                                                                gCTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCg  <  2:176658/151‑1 (MQ=255)                                                                                                                                                 cTTCTCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTc   >  1:151873/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATTTAATGCCACCAGGACCGTCGCGGCATTGGATGAACCACCGCCGAGACCGCCGCCCATCGGCAAACGCTTGTCAATGCTGATATTCGCACCGCTTCCCGTCGGAAGACGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCG  >  NC_000913/1262471‑1262764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑