Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,386,502 A→G D331G (GAT→GGT)  ycjF → UPF0283 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,386,5020AG98.5% 241.1 / ‑4.6 65D331G (GAT→GGT) ycjFUPF0283 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (24/40);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGTCGCAAGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTG  >  NC_000913/1386358‑1386646
                                                                                                                                                |                                                                                                                                                
tGTCGCAAGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCcg                                                                                                                                            >  1:889071/1‑151 (MQ=255)
 gTCGCAAGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCcg                                                                                                                                            <  2:308343/150‑1 (MQ=255)
  tCGCAAGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCcgtc                                                                                                                                          >  2:200148/1‑151 (MQ=255)
      aaGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCgtcgtc                                                                                                                                       >  2:726981/1‑150 (MQ=255)
      aaGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCa                                                                                                                                      <  2:770842/151‑1 (MQ=255)
             cGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGTGTTTCCGTCGTCAGCtt                                                                                                                                  <  1:553997/148‑1 (MQ=255)
             cGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCt                                                                                                                                   <  2:974232/147‑1 (MQ=255)
              gctgctCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCg                                                                                                                              <  2:21915/151‑1 (MQ=255)
               ctgctCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCgg                                                                                                                             >  2:451261/1‑151 (MQ=255)
                tgctCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGt                                                                                                                            >  2:489392/1‑151 (MQ=255)
                  ctCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTc                                                                                                                           >  1:1056474/1‑150 (MQ=255)
                        tGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTga                                                                                                                     <  2:399607/150‑1 (MQ=255)
                             aCCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTgaaag                                                                                                                  <  2:471291/148‑1 (MQ=255)
                                 gcgcAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCt                                                                                                           <  1:69376/151‑1 (MQ=255)
                                      gCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCaa                                                                                                       >  1:1160835/1‑150 (MQ=255)
                                       cTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCaaa                                                                                                      >  1:386677/1‑150 (MQ=255)
                                         cAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAg                                                                                                   <  2:941617/151‑1 (MQ=255)
                                            gggATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCa                                                                                                <  1:570911/151‑1 (MQ=255)
                                             ggATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTc                                                                                                                           <  2:1038418/123‑1 (MQ=255)
                                             ggATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTc                                                                                                                           >  1:1038393/1‑123 (MQ=255)
                                               aTTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCaaaa                                                                                             <  2:650550/151‑1 (MQ=255)
                                                 tGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACg                                                                                           >  2:872326/1‑151 (MQ=255)
                                                  ggTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACg                                                                                           <  2:25377/150‑1 (MQ=255)
                                                      cAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAg                                                                                      <  1:722614/151‑1 (MQ=255)
                                                       aGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGc                                                                                     <  2:729154/151‑1 (MQ=255)
                                                       aGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGc                                                                                     <  1:1007883/151‑1 (MQ=255)
                                                         gACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCg                                                                                    >  1:1025077/1‑150 (MQ=255)
                                                             tCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGaaaa                                                                                >  1:238324/1‑150 (MQ=255)
                                                             tCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGaaa                                                                                 <  2:49655/149‑1 (MQ=255)
                                                                   ggCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGc                                                                         <  2:184632/151‑1 (MQ=255)
                                                                    gCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGc                                                                         >  1:488292/1‑150 (MQ=255)
                                                                    gCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCa                                                                        <  1:1063313/151‑1 (MQ=255)
                                                                     cACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCa                                                                        >  1:1111771/1‑150 (MQ=255)
                                                                       cGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAat                                                                      >  1:406201/1‑150 (MQ=255)
                                                                         aCTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATc                                                                   <  1:872312/151‑1 (MQ=255)
                                                                           tCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCgg                                                                 >  1:57825/1‑151 (MQ=255)
                                                                            cGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCggg                                                                <  1:405362/151‑1 (MQ=255)
                                                                             gggATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGt                                                               <  2:1043123/151‑1 (MQ=255)
                                                                              ggATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTg                                                              <  1:711494/151‑1 (MQ=255)
                                                                              ggATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTg                                                              <  1:331569/151‑1 (MQ=255)
                                                                              ggATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTg                                                              >  1:376607/1‑151 (MQ=255)
                                                                                   aaaGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGAc                                                         <  1:527080/151‑1 (MQ=255)
                                                                                   aaaGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGAc                                                         >  2:286788/1‑151 (MQ=255)
                                                                                            gAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCt                                                <  2:357346/151‑1 (MQ=255)
                                                                                             aGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCtt                                               >  2:112494/1‑151 (MQ=255)
                                                                                              gCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCtt                                               <  1:4409/150‑1 (MQ=255)
                                                                                               cTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCATGTGAAAGAAACGCTGCAAAAAGGCAAAACGACCAGCGAAAGATAATGCAATATCGGGTGCTGACCGTATATCttt                                              <  1:78196/150‑1 (MQ=255)
                                                                                                 ttGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTa                                             <  1:814954/149‑1 (MQ=255)
                                                                                                 ttGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTa                                             <  2:277685/149‑1 (MQ=255)
                                                                                                  tGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTa                                             <  1:451258/148‑1 (MQ=255)
                                                                                                  tGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACg                                           <  1:1064383/150‑1 (MQ=255)
                                                                                                             gCCGTGGATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCg                               >  1:935323/1‑151 (MQ=255)
                                                                                                                   gATTGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTc                         <  2:882575/151‑1 (MQ=255)
                                                                                                                      tGACGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGt                      >  2:18534/1‑151 (MQ=255)
                                                                                                                        aCGATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCt                    <  1:200146/151‑1 (MQ=255)
                                                                                                                          gATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCttt                  >  2:593926/1‑151 (MQ=255)
                                                                                                                          gATGACAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCttt                  <  1:372578/151‑1 (MQ=255)
                                                                                                                              aCAAACCTCGCCTCGGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTgtgt              >  1:1188075/1‑151 (MQ=255)
                                                                                                                                   cctcgcctcgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATg         <  2:448760/151‑1 (MQ=255)
                                                                                                                                   cctcgcctcgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATg         <  1:826263/151‑1 (MQ=255)
                                                                                                                                      cgcctcgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAtt      <  1:139564/151‑1 (MQ=255)
                                                                                                                                      cgcctcgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAt       <  1:490440/150‑1 (MQ=255)
                                                                                                                                           cgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttgttg  >  2:867467/1‑150 (MQ=255)
                                                                                                                                           cgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttgttg  <  1:382195/150‑1 (MQ=255)
                                                                                                                                           cgGGGGTTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGAttgtt   <  2:545286/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGTCGCAAGATCTCGCTGCTCGTTTGTCTACCCGCGCAGCTCAGGGGATTGGTGCAGGACTTCTGACGGCACGACTCGGGATTAAAGCTATGGAGCTTTGCCGCCCGCTGCCGTGGATTGACGATGACAAACCTCGCCTCGGGGATTTCCGTCGTCAGCTTATCGGTCAGGTGAAAGAAACGCTGCAAAAAGGCAAAACGCCCAGCGAAAAATAATGCAATATCGGGTGCTGACCGGATATCTTTACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTG  >  NC_000913/1386358‑1386646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: