Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,483	C→T	A288V (GCG→GTG)	accA →	acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,483	0	C	T	100.0%	313.2 / NA	80	A288V (GCG→GTG)	accA	acetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/37);  total (43/37)

AGCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTT  >  NC_000913/209338‑209624                                                                                                                                                  |                                                                                                                                              agCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAAc                                                                                                                                          <  1:772295/151‑1 (MQ=255)   cgcCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAActg                                                                                                                                        >  1:1124213/1‑151 (MQ=255)     ccGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAActgct                                                                                                                                      >  2:1135923/1‑151 (MQ=255)     ccGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTAAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAActgct                                                                                                                                      <  2:950034/151‑1 (MQ=255)      cGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAActgctg                                                                                                                                     <  2:35595/151‑1 (MQ=255)         cAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCg                                                                                                                                  <  1:1075946/151‑1 (MQ=255)          aaaGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCgg                                                                                                                                 >  1:764885/1‑151 (MQ=255)            aGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGAt                                                                                                                               >  1:484483/1‑151 (MQ=255)              cgccgcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCt                                                                                                                             <  2:589889/151‑1 (MQ=255)               gccgcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTg                                                                                                                            <  1:208048/151‑1 (MQ=255)                ccgcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTgg                                                                                                                           <  1:82681/151‑1 (MQ=255)                 cgcTGGCGGCTGAAGCGATGGGAATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGc                                                                                                                          >  1:175179/1‑151 (MQ=255)                  gcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGcc                                                                                                                         >  1:432691/1‑151 (MQ=255)                            gAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGAc                                                                                                                <  2:1179473/150‑1 (MQ=255)                            gAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACg                                                                                                               <  2:1016264/151‑1 (MQ=255)                              aGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACgtg                                                                                                             >  1:320594/1‑151 (MQ=255)                                     ggTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCa                                                                                                      <  2:111432/151‑1 (MQ=255)                                          cATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGa                                                                                                  <  2:818463/150‑1 (MQ=255)                                          cATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGa                                                                                                  <  1:459452/150‑1 (MQ=255)                                           aTTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAg                                                                                                <  1:458626/151‑1 (MQ=255)                                             tGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGa                                                                                                  >  2:294135/1‑147 (MQ=255)                                                  cgcgTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTaaa                                                                                         <  1:57646/151‑1 (MQ=255)                                                   gcgTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTaaa                                                                                         >  2:86946/1‑150 (MQ=255)                                                        tGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAAtcg                                                                                   >  1:1150554/1‑151 (MQ=255)                                                        tGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAAtcg                                                                                   >  2:628966/1‑151 (MQ=255)                                                         gAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAAtcgt                                                                                  <  2:385674/151‑1 (MQ=255)                                                         gAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAAt                                                                                     <  2:846141/148‑1 (MQ=255)                                                          aaaGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAAtcgtc                                                                                 >  1:825443/1‑151 (MQ=255)                                                            aGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATcgtcgt                                                                               >  1:460904/1‑151 (MQ=255)                                                              aactgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGtt                                                                              >  2:313119/1‑150 (MQ=255)                                                              aactgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGtt                                                                              >  1:163449/1‑150 (MQ=255)                                                              aactgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGtt                                                                              >  1:838905/1‑150 (MQ=255)                                                                 tgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                           >  2:194777/1‑150 (MQ=255)                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                           >  1:395602/1‑149 (MQ=255)                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                           >  1:589435/1‑149 (MQ=255)                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCa                                                                          <  1:990703/150‑1 (MQ=255)                                                                   aaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAgc                                                                        <  2:705868/151‑1 (MQ=255)                                                                    aactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAgcg                                                                       >  1:1162839/1‑151 (MQ=255)                                                                        gaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtg                                                                   >  1:266359/1‑151 (MQ=255)                                                                        gaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtg                                                                   <  1:110551/151‑1 (MQ=255)                                                                        gaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtg                                                                   >  2:782935/1‑151 (MQ=255)                                                                          tCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtgat                                                                 >  2:788298/1‑151 (MQ=255)                                                                             aCTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAg                                                              <  1:295633/151‑1 (MQ=255)                                                                                ccATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCt                                                            <  2:101482/150‑1 (MQ=255)                                                                                ccATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCt                                                            >  1:865476/1‑150 (MQ=255)                                                                                ccATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTa                                                           <  2:450297/151‑1 (MQ=255)                                                                                 catcatCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTAc                                                          <  2:1137880/151‑1 (MQ=255)                                                                                    catcCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAGTCGTCGTTATCAGCGCCTGATGAGCTACGGt                                                       >  2:720087/1‑151 (MQ=255)                                                                                     atcCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGtt                                                      >  1:538608/1‑151 (MQ=255)                                                                                      tcCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGtt                                                      >  2:575869/1‑150 (MQ=255)                                                                                        ccGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTAcg                                                   <  2:589444/151‑1 (MQ=255)                                                                                         cGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTAcgc                                                  <  1:413822/151‑1 (MQ=255)                                                                                            aaCCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGt                                                >  1:514483/1‑150 (MQ=255)                                                                                                aCTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTc                                           >  2:809212/1‑151 (MQ=255)                                                                                                 cTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCg                                          >  1:906680/1‑151 (MQ=255)                                                                                                   gggTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGc                                         >  2:1019119/1‑150 (MQ=255)                                                                                                   gggTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGc                                         >  1:158818/1‑150 (MQ=255)                                                                                                   gggTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCa                                        <  1:569846/151‑1 (MQ=255)                                                                                                           cTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCt                                >  2:925729/1‑151 (MQ=255)                                                                                                               ccGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGaaa                            <  2:659558/151‑1 (MQ=255)                                                                                                               ccGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGaaa                            <  2:981684/151‑1 (MQ=255)                                                                                                                 gTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGaaaa                           >  1:968672/1‑150 (MQ=255)                                                                                                                  tAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGaaaa                           >  1:1067404/1‑149 (MQ=255)                                                                                                                   aaCCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAgg                        >  1:309749/1‑151 (MQ=255)                                                                                                                    aCCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAggg                       <  2:552928/151‑1 (MQ=255)                                                                                                                      ccGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTc                     <  1:635231/151‑1 (MQ=255)                                                                                                                      ccGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTc                     <  2:484486/151‑1 (MQ=255)                                                                                                                        ggAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCAc                   <  1:414342/151‑1 (MQ=255)                                                                                                                          aaGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACtt                 >  1:843128/1‑151 (MQ=255)                                                                                                                           aGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTc                <  1:787049/151‑1 (MQ=255)                                                                                                                           aGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTc                <  2:846733/151‑1 (MQ=255)                                                                                                                            gCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTc                <  1:601808/150‑1 (MQ=255)                                                                                                                            gCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGAGTCACTTCg               >  2:16536/1‑151 (MQ=255)                                                                                                                               aTGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTg            >  2:2355/1‑151 (MQ=255)                                                                                                                               aTGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTg            <  1:442277/151‑1 (MQ=255)                                                                                                                               aTGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTg            <  1:394167/151‑1 (MQ=255)                                                                                                                                  gcggcATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGcc         >  2:386076/1‑151 (MQ=255)                                                                                                                                  gcggcATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGcc         >  1:154136/1‑151 (MQ=255)                                                                                                                                     gcATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtt      >  1:910088/1‑151 (MQ=255)                                                                                                                                          gTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttttt  >  2:1156789/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                              AGCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTT  >  NC_000913/209338‑209624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑