Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,710	T→C	intergenic (‑324/+224)	sufA ← / ← ydiH	Fe‑S cluster assembly protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,710	0	T	C	98.5%	225.5 / NA	66	intergenic (‑324/+224)	sufA/ydiH	Fe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (39/26);  minor base A (0/1);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGAT  >  NC_000913/1764562‑1764853                                                                                                                                                       |                                                                                                                                                gTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGctt                                                                                                                                               <  1:69146/151‑1 (MQ=255) gTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGctt                                                                                                                                               <  2:1140368/151‑1 (MQ=255)                ttatCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGgcg                                                                                                                                >  2:144273/1‑151 (MQ=255)                      aaGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTAtc                                                                                                                          >  2:98902/1‑151 (MQ=255)                      aaGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTAtc                                                                                                                          >  2:834627/1‑151 (MQ=255)                      aaGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTAt                                                                                                                           <  2:568662/150‑1 (MQ=255)                            cATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGTGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGc                                                                                                                    >  2:413584/1‑151 (MQ=255)                             aTTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCa                                                                                                                   <  1:1008781/151‑1 (MQ=255)                              ttAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCAc                                                                                                                  >  1:59322/1‑151 (MQ=255)                               tAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCAct                                                                                                                 >  1:204794/1‑151 (MQ=255)                                aaGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCActc                                                                                                                >  1:936139/1‑151 (MQ=255)                                   gCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                                                                                              >  1:1060888/1‑150 (MQ=255)                                     tgtgATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGt                                                                                                           >  1:209393/1‑151 (MQ=255)                                        gatgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGc                                                                                                        >  1:357924/1‑151 (MQ=255)                                         atgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCt                                                                                                       <  2:566670/151‑1 (MQ=255)                                           gaaaaagaaaaaGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                                                                                      <  2:483618/150‑1 (MQ=255)                                            aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                                                                                      >  1:205701/1‑149 (MQ=255)                                            aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                                                                                      >  2:984318/1‑149 (MQ=255)                                             aaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGc                                                                                                   <  2:999415/151‑1 (MQ=255)                                                 gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCtt                                                                                               >  1:882180/1‑151 (MQ=255)                                                  aaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTg                                                                                              >  1:205198/1‑151 (MQ=255)                                                      agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg                                                                                          <  1:204830/151‑1 (MQ=255)                                                      agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg                                                                                          >  1:596333/1‑151 (MQ=255)                                                      agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg                                                                                          >  1:614223/1‑151 (MQ=255)                                                              tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                                                                                   >  2:606342/1‑150 (MQ=255)                                                               gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATaa                                                                                  <  1:170409/150‑1 (MQ=255)                                                               gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAg                                                                                 <  1:680866/151‑1 (MQ=255)                                                                aaaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa                                                                                <  1:195617/151‑1 (MQ=255)                                                                aaaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa                                                                                <  1:214340/151‑1 (MQ=255)                                                                 aaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa                                                                               <  2:47517/151‑1 (MQ=255)                                                                     gACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataat                                                                           >  1:1072013/1‑151 (MQ=255)                                                                       cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtct                                                                         >  1:373967/1‑151 (MQ=255)                                                                           ttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt                                                                      >  2:544834/1‑150 (MQ=255)                                                                              agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaa                                                                    >  2:704893/1‑149 (MQ=255)                                                                                   tAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAc                                                             >  1:382234/1‑151 (MQ=255)                                                                                     ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                                           <  1:802942/151‑1 (MQ=255)                                                                                     ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                                           <  2:365310/151‑1 (MQ=255)                                                                                      gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccg                                                          >  2:267287/1‑151 (MQ=255)                                                                                        aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc                                                        >  1:310499/1‑151 (MQ=255)                                                                                        aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc                                                        <  2:468078/151‑1 (MQ=255)                                                                                         aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgccg                                                       >  1:210348/1‑151 (MQ=255)                                                                                          aaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                                      >  1:965175/1‑151 (MQ=255)                                                                                            aaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGttt                                                    <  2:427986/151‑1 (MQ=255)                                                                                              gTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGtttt                                                   >  1:384813/1‑150 (MQ=255)                                                                                               tGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTa                                                  >  1:341279/1‑150 (MQ=255)                                                                                               tGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTa                                                  >  2:701023/1‑150 (MQ=255)                                                                                                gCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                                >  2:603495/1‑151 (MQ=255)                                                                                                        cAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTg                                        >  1:202667/1‑151 (MQ=255)                                                                                                         aaaaaTAAAAAACCCTTTAAT‑TCTGGGCGACACA‑GCTTAAGGGGCTTCTCCCCCACCCGCCTTTTATCTCCGCCCCTCTCGGTGGCTTAGCTTTGGAAGGGGCAAAAAAAATAATCCCAAAAAGCTACCCCGCCGTTTTAACACAAACTg                                        <  1:577819/148‑1 (MQ=255)                                                                                                            aaTAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAt                                    >  2:183805/1‑151 (MQ=255)                                                                                                            aaTAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAt                                    >  2:970076/1‑151 (MQ=255)                                                                                                            aaTAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAt                                    >  1:276660/1‑151 (MQ=255)                                                                                                              tAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                                   >  1:465204/1‑150 (MQ=255)                                                                                                               aaaaaacCCCTTAGG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTCCCCCATCCGGCGTTTATCTCCGGCCCTCTCAGTGGCTTAGCTCTTGAAGGGGCAATAAGAATAATCTCAAAAACCTACCCCGCCGTTTTAACACAAACTGCGATTa                                  <  2:526707/147‑1 (MQ=255)                                                                                                                    aaCCCCTAAGTTCGGGGCGACACGGGCTTAA‑GGGCTTCTCCCCCACCCGGCGTTTATCCCCGCCACTCTCAGTGGTTTGGCTTTTGAGGGGGCAATAAAAATAATCTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGtatt                             <  1:529715/151‑1 (MQ=255)                                                                                                                     aCCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtattat                           >  1:244072/1‑151 (MQ=255)                                                                                                                         ttAAG‑TCTGTGCGACACAGGCTTAA‑GGGCTTCTCCCCCATCCGGCGCTTATCCCCGGCCCTCTCAGTGGTTTAGCTCTTGAAGGGGCAATAAGAATAATCTAATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTattatt                          <  2:279797/148‑1 (MQ=255)                                                                                                                            agttCTGGGCGACACAGGCTAAA‑GGCCTTCTCCCCCACCCGGCTTTTATCCCCGCCCCTCTCAGTGTTTTAGTTTTTAAGGGGGAAATAAAAATATTTTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg                      <  1:962314/147‑1 (MQ=255)                                                                                                                            aG‑TTTGTGCGACACGGGCTAAA‑GGGCTTCTCCCCAACCCGGGCTTTCTCCCCGGCCCTTTCGGTGGTTTAGTTCTTTAGGGGGCGATAAGAAGAATCCAAAAAAGAAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa                    <  2:729944/151‑1 (MQ=255)                                                                                                                               tCTGTGCGACACAGGCTTAA‑GGTCTTCTCCCCCATCGGGCTTTTATCCCCGCCACTCTATTTGGCTTAGTTTTTGAAGGGGGAAAAAAAATAATCTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAAc                   <  1:83496/150‑1 (MQ=255)                                                                                                                                cTGTGCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACa                  >  1:35240/1‑150 (MQ=255)                                                                                                                                 tgtgCGACACAGGCTTAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAat                >  2:854880/1‑151 (MQ=255)                                                                                                                                  gtgCGACACAGGCTTAG‑GGGCTTCTCCCCCATCCGGCGTTTACCTCCGCCACTCTCAGTGGTTTGGCTTTGGAAGGGGCGATAAGAAAAACCTCAGAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAata               <  2:165288/151‑1 (MQ=255)                                                                                                                                     cGACACAGGCTAAA‑GGGCTTCTCCCCCACCCGGCGTTTATCCCCGCCACTCTCAGTGGCTTAGCTTTTGAAGGGGCAATAAAAATAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAatat              <  1:183803/149‑1 (MQ=255)                                                                                                                                        cacgGGTTTAG‑GGGCTTCTCCCCCACCCGCCGCTTATCCCCGCCACTCTCGGTGGCTTGGCTCTTGAGGGGGCGATAAAAATATTCTCATAAAGTAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAgg          <  2:856539/150‑1 (MQ=255)                                                                                                                                         acaGGCTTAA‑GGGATTCTACCCCTCCCGGCTTTTATCCCCGGCACCCTAAGTGCTTTAGCTGTTGAAGGGCGAAAAAGAATAATCCTATAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGc         <  2:65719/150‑1 (MQ=255)                                                                                                                                            ggtttAA‑GGGCTTCTCCCCCACCCGGCTCTTATCTCCGGCCCTCTCAGTGGTTTAGCTCTTGAGGGGGCGAAAAAATAAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCgg       <  2:951532/146‑1 (MQ=255)                                                                                                                                                tAA‑GGGCTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGAt  >  1:595938/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                GTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAG‑TCTGTGCGACACAGGCTTAA‑GGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGAT  >  NC_000913/1764562‑1764853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑