Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,901,862	T→C	intergenic (‑277/‑186)	yoaE ← / → manX	putative membrane protein/conserved protein/fused mannose‑specific PTS enzymes: IIA component/IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,862	0	T	C	100.0%	335.6 / NA	85	intergenic (‑277/‑186)	yoaE/manX	putative membrane protein/conserved protein/fused mannose‑specific PTS enzymes: IIA component/IIB component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/45);  total (40/45)

GTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCAAAAATACATCTG  >  NC_000913/1901716‑1902009                                                                                                                                                   |                                                                                                                                                    gTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTg                                                                                                                                                 <  1:1197107/151‑1 (MQ=255)   cGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCt                                                                                                                                               >  1:1075524/1‑151 (MQ=255)      tGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAAt                                                                                                                                            >  1:768407/1‑151 (MQ=255)       gACGATCTATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATc                                                                                                                                           <  2:195008/151‑1 (MQ=255)        aCGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCg                                                                                                                                          >  2:944964/1‑151 (MQ=255)           aTCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGaa                                                                                                                                        >  1:161502/1‑150 (MQ=255)              cATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGtt                                                                                                                                    <  1:706298/151‑1 (MQ=255)              cATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTGTACCTTTCGAAATCTCTGCTAATCGAAAGtt                                                                                                                                    <  2:309015/151‑1 (MQ=255)               aTACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGtt                                                                                                                                    >  1:805352/1‑150 (MQ=255)                 aCTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTaaa                                                                                                                                 <  2:467910/151‑1 (MQ=255)                    gCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAAt                                                                                                                                <  2:720099/149‑1 (MQ=255)                       ggCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACgg                                                                                                                           >  2:43201/1‑151 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGAt                                                                                                                         <  2:738163/149‑1 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGAt                                                                                                                         <  1:471619/149‑1 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATc                                                                                                                        <  2:726722/150‑1 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCt                                                                                                                       <  2:525098/151‑1 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCt                                                                                                                       <  1:155003/151‑1 (MQ=255)                           aCTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCt                                                                                                                       >  2:14986/1‑151 (MQ=255)                             tGCCCTGTACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTtc                                                                                                                     >  2:123591/1‑151 (MQ=255)                              gCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCtt                                                                                                                      <  1:580767/149‑1 (MQ=255)                              gCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCtt                                                                                                                      <  1:692193/149‑1 (MQ=255)                              gCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCtt                                                                                                                      <  2:472881/149‑1 (MQ=255)                              gCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTtca                                                                                                                    <  2:866311/151‑1 (MQ=255)                              gCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTtc                                                                                                                     >  1:768776/1‑150 (MQ=255)                                   ataCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                                                                                                               <  2:1034665/151‑1 (MQ=255)                                    taCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACAt                                                                                                              >  1:115907/1‑151 (MQ=255)                                       tCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATaa                                                                                                            >  1:670828/1‑150 (MQ=255)                                        ccATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATaaaa                                                                                                          <  2:1074159/151‑1 (MQ=255)                                           tGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAaata                                                                                                        >  1:590991/1‑150 (MQ=255)                                             gttgttAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAAtt                                                                                                     <  1:836721/151‑1 (MQ=255)                                               tgttAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAAttt                                                                                                    <  2:640225/150‑1 (MQ=255)                                                gttAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAAtttt                                                                                                   <  1:411593/150‑1 (MQ=255)                                                gttAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAAtttt                                                                                                   <  2:1195684/150‑1 (MQ=255)                                                  tAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAAtttttt                                                                                                 <  2:1041970/150‑1 (MQ=255)                                                   aaaCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTc                                                                                                <  1:944944/150‑1 (MQ=255)                                                   aaaCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg                                                                                               >  1:769766/1‑151 (MQ=255)                                                   aaaCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg                                                                                               <  1:99590/151‑1 (MQ=255)                                                    aaCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg                                                                                               >  1:894613/1‑150 (MQ=255)                                                    aaCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGa                                                                                              <  2:945230/151‑1 (MQ=255)                                                     aCGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGat                                                                                             <  1:890879/151‑1 (MQ=255)                                                          aGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTa                                                                                        <  2:990249/151‑1 (MQ=255)                                                          aGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTa                                                                                        <  1:308489/151‑1 (MQ=255)                                                          aGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCGTCATCACATAAAATAATTTTTTCGATATCTa                                                                                        <  2:464347/151‑1 (MQ=255)                                                               aaCATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAaaat                                                                                    >  1:88751/1‑150 (MQ=255)                                                                   tatCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATc                                                                               >  2:529889/1‑151 (MQ=255)                                                                    atCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATcg                                                                              >  2:507320/1‑151 (MQ=255)                                                                      cAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATcgc                                                                             <  1:750784/150‑1 (MQ=255)                                                                      cAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATcgc                                                                             <  1:555267/150‑1 (MQ=255)                                                                        gagaCGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGaa                                                                          <  1:610867/151‑1 (MQ=255)                                                                           aCGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACg                                                                       >  1:351380/1‑151 (MQ=255)                                                                             gCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCa                                                                     <  1:327754/151‑1 (MQ=255)                                                                              ccTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGc                                                                      >  2:1054457/1‑149 (MQ=255)                                                                                     tttGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGtttt                                                             >  2:262414/1‑151 (MQ=255)                                                                                      ttGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGttttt                                                            >  2:441732/1‑151 (MQ=255)                                                                                      ttGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGtttt                                                             <  1:556373/150‑1 (MQ=255)                                                                                       tGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTg                                                           >  2:369428/1‑151 (MQ=255)                                                                                        ggCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTg                                                           <  1:302617/150‑1 (MQ=255)                                                                                          cAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGtt                                                        >  1:1043809/1‑151 (MQ=255)                                                                                           aaaGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTg                                                       <  2:969410/151‑1 (MQ=255)                                                                                             aGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTa                                                     <  2:274043/151‑1 (MQ=255)                                                                                              gATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGt                                                      >  2:1084545/1‑149 (MQ=255)                                                                                              gATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAg                                                    <  1:934716/151‑1 (MQ=255)                                                                                               aTTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGc                                                   >  1:788655/1‑151 (MQ=255)                                                                                                 ttACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGccc                                                 >  2:490241/1‑151 (MQ=255)                                                                                                   aCCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCt                                                <  1:658577/150‑1 (MQ=255)                                                                                                       tccttTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCt                                           >  2:388436/1‑151 (MQ=255)                                                                                                          tttGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGa                                         >  2:1016859/1‑150 (MQ=255)                                                                                                            tGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAAtc                                      >  1:38000/1‑151 (MQ=255)                                                                                                            tGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAAtc                                      >  1:710259/1‑151 (MQ=255)                                                                                                              cAAACGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAAtcg                                     >  1:111098/1‑150 (MQ=255)                                                                                                                 aCGAATGTGACAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAAtcgattc                                 >  2:566943/1‑151 (MQ=255)                                                                                                                          aCAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTgg                         >  1:1178208/1‑150 (MQ=255)                                                                                                                          aCAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGga                        <  1:364051/151‑1 (MQ=255)                                                                                                                           cAAGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGgac                       >  2:715600/1‑151 (MQ=255)                                                                                                                            aaGGATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGgacg                      >  2:748923/1‑151 (MQ=255)                                                                                                                              ggATATTTTACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGgacgac                    <  1:397921/151‑1 (MQ=255)                                                                                                                                    tttACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTc               <  1:425295/150‑1 (MQ=255)                                                                                                                                    tttACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTc               >  2:77728/1‑150 (MQ=255)                                                                                                                                     ttACCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCa              <  2:884704/150‑1 (MQ=255)                                                                                                                                       aCCTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCaaaa           <  2:597705/151‑1 (MQ=255)                                                                                                                                        ccTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCaaaa           >  1:854849/1‑150 (MQ=255)                                                                                                                                        ccTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGCTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCaaaa           >  1:254477/1‑150 (MQ=255)                                                                                                                                         cTTTCGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTAATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTATAGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCaa             <  2:1074461/147‑1 (MQ=255)                                                                                                                                             cGAAATCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCAAAAATACAt     >  2:760661/1‑151 (MQ=255)                                                                                                                                                aaTCTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCAAAAATACATCTg  >  1:164554/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCTAAAATAAATCGCGAAACGCAGGGGTTTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCAAAAATACATCTG  >  NC_000913/1901716‑1902009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑