Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,121,209	A→G	Y116H (TAT→CAT)	wcaJ ←	colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,121,209	0	A	G	100.0%	227.3 / NA	63	Y116H (TAT→CAT)	wcaJ	colanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

GGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATACCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAAT  >  NC_000913/2121065‑2121355                                                                                                                                                 |                                                                                                                                                   ggAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACgc                                                                                                                                              <  2:865916/151‑1 (MQ=255)   aaGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACgcca                                                                                                                                            <  2:1124249/151‑1 (MQ=255)       tctcCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACgccagcc                                                                                                                                         >  2:867313/1‑150 (MQ=255)          ccATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACgccagcca                                                                                                                                        <  2:974499/148‑1 (MQ=255)          ccATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGAt                                                                                                                                     <  1:12500/151‑1 (MQ=255)            aTCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGAttt                                                                                                                                   <  2:138465/151‑1 (MQ=255)                  aTTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGtt                                                                                                                             <  2:970200/151‑1 (MQ=255)                        ccGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCgtgt                                                                                                                       <  1:1139550/151‑1 (MQ=255)                             ggcTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGaa                                                                                                                   >  2:945780/1‑150 (MQ=255)                                tAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATc                                                                                                                >  2:249147/1‑150 (MQ=255)                                tAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGCTGCGTGTCGAAATc                                                                                                                >  2:1037150/1‑150 (MQ=255)                                  actCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCAtt                                                                                                             <  2:977189/149‑1 (MQ=255)                                    tCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCAttgt                                                                                                           >  2:1097929/1‑151 (MQ=255)                                     cccccGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCAttgtt                                                                                                          <  2:255088/151‑1 (MQ=255)                                     cccccGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCAttgtt                                                                                                          <  2:428469/151‑1 (MQ=255)                                       cccGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGa                                                                                                        <  1:68652/151‑1 (MQ=255)                                         cGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAAc                                                                                                      <  2:931484/151‑1 (MQ=255)                                          gCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACg                                                                                                     >  2:100295/1‑151 (MQ=255)                                           ccACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACg                                                                                                     <  1:867299/150‑1 (MQ=255)                                           ccACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGc                                                                                                    <  1:238860/151‑1 (MQ=255)                                            cACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGcc                                                                                                   >  2:734067/1‑151 (MQ=255)                                              ccGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGcca                                                                                                  <  1:885753/150‑1 (MQ=255)                                                      aTGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCgg                                                                                         >  2:1184821/1‑151 (MQ=255)                                                       tGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGgc                                                                                        >  1:815197/1‑151 (MQ=255)                                                             tGTTATAGCCATGATTTCGCCGCCCGCCCGCCCCAATGCGAATACACGAACGGCAAGGCGCCAGTCCGATGCTGGTCAGCGCATGCCGCGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTg                                                                                    <  1:1027918/149‑1 (MQ=255)                                                             tGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTg                                                                                    <  2:1160878/149‑1 (MQ=255)                                                                 ataGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATc                                                                              >  1:379937/1‑151 (MQ=255)                                                                  taGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATc                                                                              >  2:508194/1‑150 (MQ=255)                                                                  taGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATc                                                                              >  1:1014103/1‑150 (MQ=255)                                                                   aGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCAc                                                                            <  1:100293/151‑1 (MQ=255)                                                                     ccATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGc                                                                          <  2:200350/151‑1 (MQ=255)                                                                       aTGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCtt                                                                        >  2:982190/1‑151 (MQ=255)                                                                       aTGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCtt                                                                        >  2:80082/1‑151 (MQ=255)                                                                       aTGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCtt                                                                        >  1:334656/1‑151 (MQ=255)                                                                            tACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGt                                                                   >  2:242262/1‑151 (MQ=255)                                                                              cGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTcc                                                                 <  2:703695/151‑1 (MQ=255)                                                                               gCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTcc                                                                 >  2:1077404/1‑150 (MQ=255)                                                                                 agccagccCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGt                                                              >  2:197135/1‑151 (MQ=255)                                                                                  gccagccCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGtt                                                             <  2:1100815/151‑1 (MQ=255)                                                                                     agccCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGATCCAGTTTTg                                                          >  2:210923/1‑151 (MQ=255)                                                                                      gcccgcccCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGt                                                         <  2:289468/151‑1 (MQ=255)                                                                                       cccgcccCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGt                                                         <  1:382972/150‑1 (MQ=255)                                                                                        ccgcccCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGt                                                         >  2:300446/1‑149 (MQ=255)                                                                                        ccgcccCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGt                                                         >  1:59331/1‑149 (MQ=255)                                                                                          gcccCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGc                                                      >  1:1024799/1‑150 (MQ=255)                                                                                               aaTGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAgg                                                >  1:1120431/1‑151 (MQ=255)                                                                                                aTGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAggg                                               >  1:871114/1‑151 (MQ=255)                                                                                                               cGGCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTg                                >  1:1181071/1‑151 (MQ=255)                                                                                                                ggCAAACCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGc                               <  2:658014/151‑1 (MQ=255)                                                                                                                   aaaCCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCg                            >  1:132421/1‑151 (MQ=255)                                                                                                                    aaCCACCAGTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGa                           <  2:428181/151‑1 (MQ=255)                                                                                                                            gTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACcgcg                   <  2:9633/151‑1 (MQ=255)                                                                                                                            gTCCGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACcgcg                   >  1:270738/1‑151 (MQ=255)                                                                                                                               cGATGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGcc                 >  2:620855/1‑150 (MQ=255)                                                                                                                                gATGCTGGTCAGCGCATGCCACGCCAGCCAGTTTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCAt               >  2:1040599/1‑151 (MQ=255)                                                                                                                                 aTGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATg              >  1:737118/1‑151 (MQ=255)                                                                                                                                 aTGCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATg              <  1:1061472/151‑1 (MQ=255)                                                                                                                                   gCTGGTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAg            <  1:383614/151‑1 (MQ=255)                                                                                                                                      ggTCAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCg          >  2:1087549/1‑150 (MQ=255)                                                                                                                                         cAGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATa       >  2:305231/1‑150 (MQ=255)                                                                                                                                          aGCGCATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATaa      >  2:75607/1‑150 (MQ=255)                                                                                                                                           gcgcATGCCACGCCAGCCAGATTTTCAGCTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATaaaa    <  1:1037125/151‑1 (MQ=255)                                                                                                                                             gcATGCCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAAt  >  1:833974/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATACCACGCCAGCCAGATTTTCAGTTGCGTGTCGAAATCATTGTTGAACGCCACCAGTCCGGCGCTGAAAATCACGCTTAAGGTCCAGTTTTGTAGCAACAGGGCAAATTCTGTCGCTGCCCGAACACCGCGCCATGAGCGATAAAAAT  >  NC_000913/2121065‑2121355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑