Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,293	A→G	D231D (GAT→GAC)	yegI ←	protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,293	0	A	G	100.0%	266.4 / NA	71	D231D (GAT→GAC)	yegI	protein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/34);  total (37/34)

TGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCATCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGCTCTGGCGGCGTAA  >  NC_000913/2150149‑2150438                                                                                                                                                 |                                                                                                                                                  tGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCag                                                                                                                                               >  2:119220/1‑149 (MQ=255)    ctgctgAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATTCGTCTCCAGCGCATTACCCGCGTCAGAGAt                                                                                                                                           >  1:1195091/1‑150 (MQ=255)      gctgAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATaa                                                                                                                                         <  2:138610/150‑1 (MQ=255)       ctgAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCAAGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGc                                                                                                                                       >  1:870635/1‑151 (MQ=255)              aTGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCacac                                                                                                                                >  2:1014933/1‑151 (MQ=255)               tGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACAcc                                                                                                                               >  2:110548/1‑151 (MQ=255)                ggCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACAcc                                                                                                                               <  1:304314/150‑1 (MQ=255)                ggCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACAcc                                                                                                                               >  2:361977/1‑150 (MQ=255)                   ttCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGa                                                                                                                            >  1:767226/1‑150 (MQ=255)                    tCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAAt                                                                                                                          >  1:889243/1‑151 (MQ=255)                      aaCATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATaa                                                                                                                        <  2:110513/151‑1 (MQ=255)                       aCATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATa                                                                                                                         >  2:835990/1‑149 (MQ=255)                       aCATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGATAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATa                                                                                                                         >  1:133877/1‑149 (MQ=255)                          tCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGAt                                                                                                                    >  1:13486/1‑151 (MQ=255)                              tCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATgccg                                                                                                                >  2:29051/1‑151 (MQ=255)                                        cGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCaaa                                                                                                       <  2:38070/150‑1 (MQ=255)                                        cGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCaaa                                                                                                       <  2:939436/150‑1 (MQ=255)                                               ggAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGt                                                                                               >  1:836842/1‑151 (MQ=255)                                                gAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTg                                                                                              <  1:336751/151‑1 (MQ=255)                                                  aTAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGaa                                                                                            <  1:1122773/151‑1 (MQ=255)                                                   tAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGaa                                                                                            >  2:961897/1‑150 (MQ=255)                                                    aGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGaaa                                                                                           <  2:175572/150‑1 (MQ=255)                                                    aGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCCCGTCAGAGATAAGCGGCACACCAGAATAACGATGCCGCCCACCAAACAACACGTGaaaa                                                                                          <  1:53781/151‑1 (MQ=255)                                                      aTCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAAt                                                                                        <  2:13187/151‑1 (MQ=255)                                                         gTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAAtt                                                                                       >  2:288104/1‑149 (MQ=255)                                                             cggcggTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAAc                                                                                 <  1:911765/151‑1 (MQ=255)                                                             cggcggTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAAc                                                                                 <  2:1120664/151‑1 (MQ=255)                                                               gcggTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGc                                                                               >  1:578785/1‑151 (MQ=255)                                                                  gTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCa                                                                              >  1:848950/1‑149 (MQ=255)                                                                    tttAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAgg                                                                           <  2:626070/150‑1 (MQ=255)                                                                    tttAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAgg                                                                           <  1:925362/150‑1 (MQ=255)                                                                    tttAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGc                                                                          >  1:593395/1‑151 (MQ=255)                                                                      tAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGcc                                                                         <  2:492649/150‑1 (MQ=255)                                                                       aaaCCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAAAGCAAGGCCa                                                                        >  1:247390/1‑150 (MQ=255)                                                                         aCCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCaaa                                                                      >  2:184526/1‑150 (MQ=255)                                                                          ccACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAAt                                                                    <  1:940704/151‑1 (MQ=255)                                                                           cACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCTCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAAtt                                                                   <  2:657534/151‑1 (MQ=255)                                                                           cACGTCGCTGATTATCTGACCCGTAGGCATAACGGAAATGGGTAATATCCGTATCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGAGAAAAATTAGCAACGCAAGGCCAAAAtt                                                                   >  2:580225/1‑151 (MQ=255)                                                                               tCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCg                                                               >  2:527133/1‑151 (MQ=255)                                                                                   tGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGGATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGAt                                                           >  2:886469/1‑151 (MQ=255)                                                                                    gATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTTAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGAtt                                                          <  1:721971/151‑1 (MQ=255)                                                                                    gATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGAtt                                                          <  2:49000/151‑1 (MQ=255)                                                                                     aTTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGAttt                                                         >  2:114173/1‑151 (MQ=255)                                                                                        aTCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTc                                                       <  2:83827/150‑1 (MQ=255)                                                                                           tGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCtgtg                                                   >  1:562956/1‑151 (MQ=255)                                                                                            gACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGc                                                  <  2:194175/151‑1 (MQ=255)                                                                                            gACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGc                                                  <  2:757159/151‑1 (MQ=255)                                                                                              cgcgTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGt                                                <  1:154080/151‑1 (MQ=255)                                                                                                 gTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTc                                              >  2:395065/1‑150 (MQ=255)                                                                                                 gTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCa                                             <  1:114981/151‑1 (MQ=255)                                                                                                 gTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCACCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGACCAAAATTATCGTGATTTTCTGTGCGTTCa                                             <  1:836520/151‑1 (MQ=255)                                                                                                  tAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCa                                             >  2:559704/1‑150 (MQ=255)                                                                                                   aGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCaaa                                           <  1:662259/151‑1 (MQ=255)                                                                                                   aGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCaa                                            <  1:1119156/150‑1 (MQ=255)                                                                                                   aGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCaa                                            >  1:5437/1‑150 (MQ=255)                                                                                                     gCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAAc                                         >  2:682009/1‑151 (MQ=255)                                                                                                         aaCGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGa                                      <  2:827376/150‑1 (MQ=255)                                                                                                         aaCGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGAc                                     >  1:140603/1‑151 (MQ=255)                                                                                                         aaCGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGAc                                     >  2:582083/1‑151 (MQ=255)                                                                                                           cGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACa                                    >  1:511485/1‑150 (MQ=255)                                                                                                            ggAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACaa                                   >  1:1100385/1‑150 (MQ=255)                                                                                                             gAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAat                                 <  2:757544/151‑1 (MQ=255)                                                                                                                 tGGGTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAatgatg                             >  2:213098/1‑151 (MQ=255)                                                                                                                  gggTAATATCCGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAatgatg                             <  1:606911/150‑1 (MQ=255)                                                                                                                            cGTCTCCAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGt                  >  2:1085520/1‑151 (MQ=255)                                                                                                                               cttcAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGc               >  2:1172054/4‑151 (MQ=255)                                                                                                                                 ccAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAg                <  1:147430/148‑1 (MQ=255)                                                                                                                                 ccAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGctc             <  2:644575/151‑1 (MQ=255)                                                                                                                                  cAGCGCATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGctct            <  2:492353/151‑1 (MQ=255)                                                                                                                                       cATTACCCGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGCTCTggcgg       >  1:424145/1‑151 (MQ=255)                                                                                                                                            cccGCGTCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGCTCTGGCGGCGTaa  <  2:593404/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGGTAATATCCGTCTCCAGCGCATTACCCGCATCAGAGATAAGCGGCACACCAGAATAAGGATGCCGCCCACCAAACAAGACGTGAAAAATTAGCAACGCAAGGCCAAAATTATCGTGATTTTCTGTGCGTTCAAAACCGACAAATGATGGCAAGGTTTGTAGCTCTGGCGGCGTAA  >  NC_000913/2150149‑2150438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑