Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,101	G→A	R89C (CGC→TGC)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,101	0	G	A	100.0%	277.3 / NA	72	R89C (CGC→TGC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

CTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTAC  >  NC_000913/2261953‑2262247                                                                                                                                                     |                                                                                                                                                   cTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGC‑TAAAGTTAATTCGGGGGCAccc                                                                                                                                                 >  1:141076/1‑151 (MQ=255)     cacaGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTg                                                                                                                                              >  1:838152/1‑151 (MQ=255)         gACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGCGACTTCAAAACCCGAGAAGCTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGCGCACCCCt                                                                                                                                               >  1:28726/1‑146 (MQ=255)               atCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTgg                                                                                                                                    >  2:1040017/1‑151 (MQ=255)                 cGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGaa                                                                                                                                  <  1:382397/151‑1 (MQ=255)                   aaCTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAAc                                                                                                                                <  1:457182/151‑1 (MQ=255)                       gACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGt                                                                                                                             <  1:780243/150‑1 (MQ=255)                          cGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGc                                                                                                                         <  1:700844/151‑1 (MQ=255)                             gCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAAt                                                                                                                      <  1:1156712/151‑1 (MQ=255)                               cAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCAGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGc                                                                                                                    >  2:788717/1‑151 (MQ=255)                                 gCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGccc                                                                                                                  <  1:1120476/151‑1 (MQ=255)                                   tCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGccc                                                                                                                  >  1:1169364/1‑149 (MQ=255)                                    cAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCa                                                                                                                 <  2:407244/149‑1 (MQ=255)                                    cAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAg                                                                                                                <  2:157023/150‑1 (MQ=255)                                      gagaATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTc                                                                                                             <  2:441481/151‑1 (MQ=255)                                         aaTCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCt                                                                                                          <  2:268857/151‑1 (MQ=255)                                           tCAGTCACAAAGCGTACCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTg                                                                                                         >  1:1012445/1‑150 (MQ=255)                                            cAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGa                                                                                                        <  1:555197/150‑1 (MQ=255)                                               tCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAAc                                                                                                      >  1:283083/1‑149 (MQ=255)                                               tCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAAc                                                                                                      >  1:1104022/1‑149 (MQ=255)                                                cacaAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAg                                                                                                    <  1:222375/150‑1 (MQ=255)                                                  caAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGTGAAGGTGAAGTCGGTCACTTCGCCGTCTTTTTCAGTCAGCTTAACGTTAATTCTGGTGAACCGCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGtt                                                                                                  >  1:1089448/1‑150 (MQ=255)                                                  caAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTg                                                                                                 <  1:1082277/151‑1 (MQ=255)                                                   aaaGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGc                                                                                                >  1:522481/1‑151 (MQ=255)                                                     aGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTg                                                                                              >  1:283707/1‑151 (MQ=255)                                                           cccAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAAc                                                                                         <  1:1039992/150‑1 (MQ=255)                                                              aGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATc                                                                                     >  2:266302/1‑151 (MQ=255)                                                              aGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATc                                                                                     <  1:270304/151‑1 (MQ=255)                                                                 cggcggGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCt                                                                                   >  1:1080342/1‑150 (MQ=255)                                                                 cggcggGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGGGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCt                                                                                   >  1:134468/1‑150 (MQ=255)                                                                  ggcggGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGa                                                                                 <  2:691972/151‑1 (MQ=255)                                                                     gggggTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTg                                                                              <  1:855492/151‑1 (MQ=255)                                                                           gACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTa                                                                        <  1:621836/151‑1 (MQ=255)                                                                             cTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTAcc                                                                      >  1:856688/1‑151 (MQ=255)                                                                                cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTa                                                                        <  1:390626/146‑1 (MQ=255)                                                                                cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTa                                                                        >  2:390629/1‑146 (MQ=255)                                                                                cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTAccc                                                                     >  2:81731/1‑149 (MQ=255)                                                                                   aaCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGa                                                                 >  1:555412/1‑150 (MQ=255)                                                                                    aCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAg                                                               <  2:650694/151‑1 (MQ=255)                                                                                      ccGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAgcc                                                             >  2:971072/1‑151 (MQ=255)                                                                                       cGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAgccg                                                            <  2:833451/151‑1 (MQ=255)                                                                                           aaGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCa                                                         >  2:906089/1‑150 (MQ=255)                                                                                                       gTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCaa                                            <  2:335881/151‑1 (MQ=255)                                                                                                            ttCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTccc                                       >  1:254488/1‑151 (MQ=255)                                                                                                             tCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTccc                                       >  2:887498/1‑150 (MQ=255)                                                                                                             tCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTccc                                       <  2:1169098/150‑1 (MQ=255)                                                                                                              cGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCa                                      <  1:108666/150‑1 (MQ=255)                                                                                                              cGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCa                                      <  1:263999/150‑1 (MQ=255)                                                                                                                ccGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGt                                   <  2:570536/151‑1 (MQ=255)                                                                                                                ccGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGt                                   >  2:1071541/1‑151 (MQ=255)                                                                                                                  gTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCt                                 <  2:911613/151‑1 (MQ=255)                                                                                                                      ttttCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCtttt                              >  2:1150008/1‑150 (MQ=255)                                                                                                                      ttttCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCtt                                <  1:212460/148‑1 (MQ=255)                                                                                                                       tttCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTa                             <  2:1090022/150‑1 (MQ=255)                                                                                                                        ttCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAAt                           >  1:810407/1‑151 (MQ=255)                                                                                                                         tCCGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAAt                           >  1:442372/1‑150 (MQ=255)                                                                                                                           cGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACt                        >  2:701146/1‑151 (MQ=255)                                                                                                                           cGTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACt                        >  1:676649/1‑151 (MQ=255)                                                                                                                            gTCAGCTTAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACtt                       >  2:545276/1‑151 (MQ=255)                                                                                                                                   tAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGcc                  <  1:1101023/149‑1 (MQ=255)                                                                                                                                   tAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGcc                  <  1:545273/149‑1 (MQ=255)                                                                                                                                   tAACGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCa                 <  2:810419/150‑1 (MQ=255)                                                                                                                                    aaCGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACg               >  1:484807/1‑151 (MQ=255)                                                                                                                                    aaCGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACg               <  2:1080369/151‑1 (MQ=255)                                                                                                                                     aCGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGt              >  1:318477/1‑151 (MQ=255)                                                                                                                                     aCGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGt              <  1:518994/151‑1 (MQ=255)                                                                                                                                      cGTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGtt             >  2:78286/1‑151 (MQ=255)                                                                                                                                       gTTAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTg            <  1:1099293/151‑1 (MQ=255)                                                                                                                                        ttAATTCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTg            >  2:891220/1‑150 (MQ=255)                                                                                                                                             tCGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGcc       <  2:660639/150‑1 (MQ=255)                                                                                                                                              cGGGTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCtt     <  1:586509/151‑1 (MQ=255)                                                                                                                                                 gTGCACCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTAc  <  1:266299/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTAC  >  NC_000913/2261953‑2262247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑