Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,375	T→C	E374G (GAG→GGG)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,375	0	T	C	100.0%	285.3 / NA	77	E374G (GAG→GGG)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/36);  total (41/36)

TGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCC  >  NC_000913/2262227‑2262520                                                                                                                                                      |                                                                                                                                                  tGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑cccccc                                                                                                                                                 >  2:542479/1‑151 (MQ=255)  ggCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑Ccccccc                                                                                                                                                >  2:106649/1‑151 (MQ=255)   gCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑cccccccc                                                                                                                                               >  1:728213/1‑151 (MQ=255)    ccACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑cccccccc                                                                                                                                               >  2:163177/1‑150 (MQ=255)     cagtttaatcccTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCaa                                                                                                                                             <  2:809898/141‑1 (MQ=255)     cACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGAAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCa                                                                                                                                              >  1:877754/1‑150 (MQ=255)      aCGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑ccccccccc                                                                                                                                              <  2:893410/149‑2 (MQ=255)         ttGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGAcc                                                                                                                                         >  2:278392/1‑151 (MQ=255)           gATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGAcc                                                                                                                                         <  1:1100367/149‑1 (MQ=255)            aTGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACcagc                                                                                                                                      >  2:363632/1‑151 (MQ=255)             tGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACcagc                                                                                                                                      >  2:278222/1‑150 (MQ=255)             tGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACcagc                                                                                                                                      >  2:1184826/1‑150 (MQ=255)               ccTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGAcc                                                                                                                                         <  1:954627/145‑1 (MQ=255)                 tttACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCg                                                                                                                                  >  1:1097266/1‑150 (MQ=255)                   tACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGAt                                                                                                                                >  1:1107930/1‑150 (MQ=255)                     cccGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGAt                                                                                                                                <  2:643784/148‑1 (MQ=255)                     cccGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGc                                                                                                                             >  1:395338/1‑151 (MQ=255)                       cgccgcATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGt                                                                                                                           >  2:816894/1‑151 (MQ=255)                            cATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCg                                                                                                                      >  1:506779/1‑151 (MQ=255)                                     cGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTc                                                                                                             <  1:918625/151‑1 (MQ=255)                                     cGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTc                                                                                                             <  2:9459/151‑1 (MQ=255)                                      ggtggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTc                                                                                                             >  2:639116/1‑150 (MQ=255)                                      ggtggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCa                                                                                                            <  2:141078/151‑1 (MQ=255)                                              tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGt                                                                                                    >  2:174359/1‑151 (MQ=255)                                               cACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGtt                                                                                                   <  2:815362/151‑1 (MQ=255)                                               cACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGt                                                                                                    <  2:639965/150‑1 (MQ=255)                                                 ccAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCa                                                                                                 <  2:176818/151‑1 (MQ=255)                                                  cAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCa                                                                                                 <  2:141691/150‑1 (MQ=255)                                                   aGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGc                                                                                               <  2:126736/151‑1 (MQ=255)                                                         aCTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGc                                                                                         >  2:234046/1‑151 (MQ=255)                                                             cgccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCa                                                                                        <  2:1151886/148‑1 (MQ=255)                                                                cgcgTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTgg                                                                                  >  1:1108309/1‑151 (MQ=255)                                                                cgcgTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTgg                                                                                  >  1:177816/1‑151 (MQ=255)                                                                 gcgTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCCCCCCCAAGACCAGCAGCGATAGCGTc                                                                                                                          <  2:436043/111‑1 (MQ=255)                                                                 gcgTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCCCCCCCAAGACCAGCAGCGATAGCGTc                                                                                                                          >  1:436040/1‑111 (MQ=255)                                                                    ttCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCgg                                                                              >  1:896550/1‑151 (MQ=255)                                                                      cAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGt                                                                             >  2:764015/1‑150 (MQ=255)                                                                       aaTTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTaa                                                                           <  1:971050/151‑1 (MQ=255)                                                                        aTTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTaa                                                                           >  2:305678/1‑150 (MQ=255)                                                                        aTTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTaa                                                                           >  2:854927/1‑150 (MQ=255)                                                                          ttCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAg                                                                         <  1:1079708/150‑1 (MQ=255)                                                                          ttCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAg                                                                         <  2:801498/150‑1 (MQ=255)                                                                                     aaCCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATg                                                              >  1:253230/1‑150 (MQ=255)                                                                                       ccAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                           >  1:165732/1‑151 (MQ=255)                                                                                       ccAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                           >  2:819634/1‑151 (MQ=255)                                                                                           caaGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTc                                                       >  2:930669/1‑151 (MQ=255)                                                                                            aaGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCt                                                      <  1:1149980/151‑1 (MQ=255)                                                                                            aaGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCt                                                      >  1:887848/1‑151 (MQ=255)                                                                                             aGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCtt                                                     <  1:24869/151‑1 (MQ=255)                                                                                              ggTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTa                                                    <  2:751333/151‑1 (MQ=255)                                                                                                    aaGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGcc                                               <  2:749023/150‑1 (MQ=255)                                                                                                    aaGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGc                                                >  1:617097/1‑149 (MQ=255)                                                                                                       ccGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAg                                            >  1:262890/1‑150 (MQ=255)                                                                                                        cGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTg                                          >  1:734398/1‑151 (MQ=255)                                                                                                        cGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTg                                          <  2:318480/151‑1 (MQ=255)                                                                                                          gATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGcc                                        <  1:250240/151‑1 (MQ=255)                                                                                                           aTTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCca                                       <  1:1106132/151‑1 (MQ=255)                                                                                                              aaGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                     >  2:159817/1‑150 (MQ=255)                                                                                                              aaGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                     <  1:104442/150‑1 (MQ=255)                                                                                                                gggTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                  >  1:355716/1‑151 (MQ=255)                                                                                                                 ggTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACtt                                 <  1:33423/151‑1 (MQ=255)                                                                                                                    gATAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtca                              <  1:78285/151‑1 (MQ=255)                                                                                                                     aTAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                             >  2:980944/1‑151 (MQ=255)                                                                                                                     aTAGTAGCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                             <  2:632663/151‑1 (MQ=255)                                                                                                                      tagtagCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                            >  2:947474/1‑151 (MQ=255)                                                                                                                       agtagCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                            >  2:1095003/1‑150 (MQ=255)                                                                                                                       agtagCAACACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                            <  1:542476/150‑1 (MQ=255)                                                                                                                             aaCACGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTAc                     >  2:171582/1‑151 (MQ=255)                                                                                                                                acGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTc                  <  1:255968/151‑1 (MQ=255)                                                                                                                                 cGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTcc                 <  2:234837/151‑1 (MQ=255)                                                                                                                                 cGTCTGCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTcc                 <  1:49659/151‑1 (MQ=255)                                                                                                                                      gCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg            <  2:1049143/151‑1 (MQ=255)                                                                                                                                      gCTCATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg            >  1:810538/1‑151 (MQ=255)                                                                                                                                         cATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgcc          <  2:195260/150‑1 (MQ=255)                                                                                                                                         cATTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgcc          >  1:982166/1‑150 (MQ=255)                                                                                                                                          aTTATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccgg        >  1:981820/1‑151 (MQ=255)                                                                                                                                            tATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt      >  1:859948/1‑151 (MQ=255)                                                                                                                                            tATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt      >  1:301501/1‑151 (MQ=255)                                                                                                                                            tATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt      >  2:992007/1‑151 (MQ=255)                                                                                                                                            tATGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggt       <  1:819620/150‑1 (MQ=255)                                                                                                                                             aTGCG‑CCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggttt     >  1:800321/1‑151 (MQ=255)                                                                                                                                                cg‑cCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttgcc  <  2:230853/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  TGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCG‑CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCC  >  NC_000913/2262227‑2262520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑