Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,782	T→C	F202L (TTC→CTC)	yejB →	microcin C ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,782	0	T	C	100.0%	283.9 / NA	76	F202L (TTC→CTC)	yejB	microcin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/45);  total (31/45)

TCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGTTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCA  >  NC_000913/2274634‑2274928                                                                                                                                                     |                                                                                                                                                   tCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTc                                                                                                                                                  >  2:934150/1‑151 (MQ=255)      tAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCctct                                                                                                                                             >  1:586995/1‑151 (MQ=255)       aGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGATGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCctc                                                                                                                                              >  2:961738/1‑149 (MQ=255)        ggCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTAc                                                                                                                                           <  2:820398/151‑1 (MQ=255)         gCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTAcg                                                                                                                                          >  1:147891/1‑151 (MQ=255)             tCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCgg                                                                                                                                       <  2:478853/150‑1 (MQ=255)               gCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCt                                                                                                                                    <  2:925434/151‑1 (MQ=255)               gCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCt                                                                                                                                    <  2:529167/151‑1 (MQ=255)               gCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCt                                                                                                                                    >  2:1168597/1‑151 (MQ=255)                cAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTg                                                                                                                                   <  2:998370/151‑1 (MQ=255)                  aaGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGt                                                                                                                                 <  1:1028130/151‑1 (MQ=255)                  aaGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGt                                                                                                                                 <  2:81397/151‑1 (MQ=255)                       gTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTcc                                                                                                                             <  1:1187226/150‑1 (MQ=255)                       gTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTc                                                                                                                              <  1:192803/149‑1 (MQ=255)                       gTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTc                                                                                                                              <  1:122655/149‑1 (MQ=255)                         ttATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGc                                                                                                                           <  1:832742/150‑1 (MQ=255)                         ttATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCt                                                                                                                          >  2:870145/1‑151 (MQ=255)                          tataATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCt                                                                                                                          <  1:1131912/150‑1 (MQ=255)                           ataATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTaa                                                                                                                        <  2:397866/151‑1 (MQ=255)                              aTGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACtt                                                                                                                     <  2:801224/151‑1 (MQ=255)                                  gAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGAt                                                                                                                 <  2:1190058/151‑1 (MQ=255)                                   aGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGAtt                                                                                                                >  2:1179956/1‑151 (MQ=255)                                       gCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCt                                                                                                            <  1:700486/151‑1 (MQ=255)                                        cTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTg                                                                                                           >  2:724694/1‑151 (MQ=255)                                          ttGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTg                                                                                                           <  2:59435/149‑1 (MQ=255)                                          ttGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGcc                                                                                                         >  1:581522/1‑151 (MQ=255)                                           tGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCg                                                                                                        >  2:546158/1‑151 (MQ=255)                                             aCGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTg                                                                                                      >  2:247116/1‑151 (MQ=255)                                               gTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGt                                                                                                    >  1:904071/1‑151 (MQ=255)                                                     aGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATc                                                                                                 >  1:81622/1‑148 (MQ=255)                                                             aTTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCGcc                                                                                      <  1:606803/151‑1 (MQ=255)                                                                  tcatcatcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGAtt                                                                                  >  2:459031/1‑150 (MQ=255)                                                                   catcatcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTAt                                                                                <  2:889651/151‑1 (MQ=255)                                                                    atcatcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATc                                                                               >  2:770477/1‑151 (MQ=255)                                                                       atcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAAGCACCGATTATCtgt                                                                            <  2:610581/151‑1 (MQ=255)                                                                        tcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCtgtg                                                                           >  1:258342/1‑151 (MQ=255)                                                                         cGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCtgtg                                                                           <  1:32338/150‑1 (MQ=255)                                                                            cTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCat                                                                       >  2:127632/1‑151 (MQ=255)                                                                            cTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCat                                                                       >  2:134450/1‑151 (MQ=255)                                                                             tACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCat                                                                       >  1:168055/1‑150 (MQ=255)                                                                                 ccATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCAc                                                                  <  2:497246/151‑1 (MQ=255)                                                                                      ccGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGc                                                                >  1:888147/1‑148 (MQ=255)                                                                                      ccGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTg                                                              <  1:134448/150‑1 (MQ=255)                                                                                      ccGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGc                                                             >  1:56239/1‑151 (MQ=255)                                                                                       cGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCTCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCt                                                               <  1:419032/148‑1 (MQ=255)                                                                                            tttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGc                                                       >  1:340667/1‑151 (MQ=255)                                                                                             ttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGc                                                       >  1:359440/1‑150 (MQ=255)                                                                                                 gtttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGc                                                   <  1:642788/150‑1 (MQ=255)                                                                                                 gtttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGc                                                   <  2:413332/150‑1 (MQ=255)                                                                                                 gtttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCg                                                  <  2:630924/151‑1 (MQ=255)                                                                                                   ttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGAc                                                >  1:1166480/1‑151 (MQ=255)                                                                                                      ccATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGt                                             >  1:731320/1‑151 (MQ=255)                                                                                                       cATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTg                                            <  2:147893/151‑1 (MQ=255)                                                                                                       cATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTACCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGt                                             >  1:233048/1‑150 (MQ=255)                                                                                                        aTCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTg                                            >  1:711954/1‑150 (MQ=255)                                                                                                             gctgATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATtgg                                       <  2:311906/150‑1 (MQ=255)                                                                                                                 aTTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCt                                  >  2:735200/1‑151 (MQ=255)                                                                                                                    gTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTg                               <  2:718485/151‑1 (MQ=255)                                                                                                                    gTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTg                               >  1:648467/1‑151 (MQ=255)                                                                                                                        tcttcGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTgc                              <  1:669369/148‑1 (MQ=255)                                                                                                                            cgcgGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCt                        <  2:340670/150‑1 (MQ=255)                                                                                                                            cgcgGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCt                        <  1:537690/150‑1 (MQ=255)                                                                                                                             gcgggcggCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTg                       >  2:656003/1‑150 (MQ=255)                                                                                                                                ggcggcAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCa                   <  2:1162071/151‑1 (MQ=255)                                                                                                                                 gcggcAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCAt                  <  2:556891/151‑1 (MQ=255)                                                                                                                                  cggcAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATg                 <  1:797597/151‑1 (MQ=255)                                                                                                                                    gcAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCt               <  2:612936/151‑1 (MQ=255)                                                                                                                                     cAGCTATTTCTACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTg              <  1:420527/151‑1 (MQ=255)                                                                                                                                      aGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGa             <  1:934131/151‑1 (MQ=255)                                                                                                                                          aTTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaa         <  1:49437/151‑1 (MQ=255)                                                                                                                                          aTTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACa           <  2:282176/149‑1 (MQ=255)                                                                                                                                            ttCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaaa        <  2:252563/150‑1 (MQ=255)                                                                                                                                             tCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaaaa       <  2:616998/150‑1 (MQ=255)                                                                                                                                             tCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaaaa       <  2:112209/150‑1 (MQ=255)                                                                                                                                              cGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAAc     >  1:723820/1‑151 (MQ=255)                                                                                                                                                 ccTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCa  <  1:913566/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGTTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTCA  >  NC_000913/2274634‑2274928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑