Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,732	A→G	T9A (ACA→GCA)	yfcH →	putative NAD‑dependent nucleotide‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,732	0	A	G	100.0%	200.2 / NA	53	T9A (ACA→GCA)	yfcH	putative NAD‑dependent nucleotide‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

AACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACC  >  NC_000913/2421590‑2421876                                                                                                                                               |                                                                                                                                                 aaCGTCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTa                                                                                                                                          <  1:25602/151‑1 (MQ=255)  aCATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTaa                                                                                                                                         <  2:1178716/151‑1 (MQ=255)   cATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAAt                                                                                                                                        <  1:1176256/151‑1 (MQ=255)     tCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTg                                                                                                                                      >  1:351222/1‑151 (MQ=255)       aCTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGt                                                                                                                                    >  1:347788/1‑151 (MQ=255)          gggTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTc                                                                                                                                   <  1:187778/149‑1 (MQ=255)            gTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGcc                                                                                                                                >  1:111854/1‑150 (MQ=255)                cGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTg                                                                                                                           <  2:523450/151‑1 (MQ=255)                 gTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGa                                                                                                                          <  2:960949/151‑1 (MQ=255)                   aTGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGAtt                                                                                                                        <  1:1025443/151‑1 (MQ=255)                      cTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCcg                                                                                                                     >  2:792492/1‑151 (MQ=255)                        gAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCcg                                                                                                                     <  1:225822/149‑1 (MQ=255)                          aGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGtt                                                                                                                 <  1:622277/151‑1 (MQ=255)                           gTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGttt                                                                                                                <  1:203879/151‑1 (MQ=255)                           gTGGAGATCGATAAACTGCACGCGCTGAGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGttt                                                                                                                >  2:1058700/1‑151 (MQ=255)                                 aTCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGa                                                                                                          <  1:248771/151‑1 (MQ=255)                                     aTAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTg                                                                                                      >  2:256040/1‑151 (MQ=255)                                       aaaCTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTggg                                                                                                    >  2:605155/1‑151 (MQ=255)                                          cTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGcc                                                                                                  >  1:662470/1‑150 (MQ=255)                                                  gctgcgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTa                                                                                         <  1:861219/151‑1 (MQ=255)                                                      cgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGt                                                                                     <  1:1105150/151‑1 (MQ=255)                                                       gctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTa                                                                                    <  1:344238/151‑1 (MQ=255)                                                         tACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  >  2:336651/1‑151 (MQ=255)                                                         tACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  >  1:164668/1‑151 (MQ=255)                                                          aCGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  <  2:828165/150‑1 (MQ=255)                                                             ccGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGAcg                                                                              >  2:193062/1‑151 (MQ=255)                                                              cGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGAcgc                                                                             <  1:1174400/151‑1 (MQ=255)                                                                   cGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTaa                                                                         <  2:125997/150‑1 (MQ=255)                                                                    ggTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAAcc                                                                       <  2:1054059/151‑1 (MQ=255)                                                                      tATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCg                                                                     >  1:509883/1‑151 (MQ=255)                                                                       aTCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGc                                                                    >  1:1163185/1‑151 (MQ=255)                                                                           aTGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGaa                                                                <  2:843537/151‑1 (MQ=255)                                                                                   aaGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGtt                                                        <  2:632651/151‑1 (MQ=255)                                                                                              gTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGc                                             >  2:11263/1‑151 (MQ=255)                                                                                                     gCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCggg                                      >  1:536779/1‑151 (MQ=255)                                                                                                      ccATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGt                                     <  1:1043764/151‑1 (MQ=255)                                                                                                            cGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACt                               >  1:896418/1‑151 (MQ=255)                                                                                                             gggAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACt                               >  1:844575/1‑150 (MQ=255)                                                                                                              ggAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTAt                             >  2:247285/1‑151 (MQ=255)                                                                                                               gAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATg                            <  1:615961/151‑1 (MQ=255)                                                                                                                 ggCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATg                            >  2:110192/1‑149 (MQ=255)                                                                                                                 ggCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGc                          <  1:339790/151‑1 (MQ=255)                                                                                                                      cATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAggg                     >  1:604288/1‑151 (MQ=255)                                                                                                                            cATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGcc               >  1:578009/1‑151 (MQ=255)                                                                                                                              tAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCg              >  1:596261/1‑150 (MQ=255)                                                                                                                               aGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGAt            <  2:111856/151‑1 (MQ=255)                                                                                                                                     cACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAg       <  1:1187024/150‑1 (MQ=255)                                                                                                                                      aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGc      >  2:393460/1‑150 (MQ=255)                                                                                                                                      aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGc      >  2:665749/1‑150 (MQ=255)                                                                                                                                      aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCa     <  2:876973/151‑1 (MQ=255)                                                                                                                                        cGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAc   >  2:372190/1‑151 (MQ=255)                                                                                                                                        cGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAc   >  2:341281/1‑151 (MQ=255)                                                                                                                                         ggAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAcc  >  1:1120035/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                 AACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACC  >  NC_000913/2421590‑2421876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑