Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,482,332	T→C	A336A (GCA→GCG)	emrK ←	multidrug resistance efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,482,332	0	T	C	100.0%	289.2 / NA	76	A336A (GCA→GCG)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

GTCCATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTTGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGCCGGTTCCC  >  NC_000913/2482184‑2482479                                                                                                                                                     |                                                                                                                                                    gTCCATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGc                                                                                                                                                   >  2:274227/1‑151 (MQ=255) gTCCATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGc                                                                                                                                                   >  2:374033/1‑151 (MQ=255) gTCCATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGc                                                                                                                                                   <  2:250892/151‑1 (MQ=255)   ccATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCt                                                                                                                                                  <  2:431269/150‑1 (MQ=255)    cATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGt                                                                                                                                                >  2:134653/1‑151 (MQ=255)     aTTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTc                                                                                                                                               <  1:230881/151‑1 (MQ=255)       tATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCa                                                                                                                                              >  1:648262/1‑150 (MQ=255)        aTGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATc                                                                                                                                            >  1:901431/1‑151 (MQ=255)        aTGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATc                                                                                                                                            <  1:915371/151‑1 (MQ=255)        aTGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATc                                                                                                                                            >  2:1071652/1‑151 (MQ=255)           cGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGAt                                                                                                                                         <  2:644544/151‑1 (MQ=255)            gAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGAt                                                                                                                                         >  1:977883/1‑150 (MQ=255)            gAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGAt                                                                                                                                         >  1:1108667/1‑150 (MQ=255)            gAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGATCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGAt                                                                                                                                         >  1:864691/1‑150 (MQ=255)             aaaTAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATa                                                                                                                                        >  1:1083890/1‑150 (MQ=255)              aataatGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATaaa                                                                                                                                      <  1:816704/151‑1 (MQ=255)              aataatGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATaa                                                                                                                                       >  2:654105/1‑150 (MQ=255)                 aatGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCa                                                                                                                                   <  1:922218/151‑1 (MQ=255)                       gCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGc                                                                                                                             <  2:668336/151‑1 (MQ=255)                       gCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGc                                                                                                                             >  2:1141994/1‑151 (MQ=255)                        cTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGc                                                                                                                             >  1:200811/1‑150 (MQ=255)                                   ttCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTcc                                                                                                                 >  1:144808/1‑151 (MQ=255)                                          ggACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGtt                                                                                                          <  2:167101/151‑1 (MQ=255)                                                   tCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGAt                                                                                                 >  2:301928/1‑151 (MQ=255)                                                    cGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATc                                                                                                >  1:287690/1‑151 (MQ=255)                                                      aTAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCaa                                                                                              <  2:565827/151‑1 (MQ=255)                                                       tAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCa                                                                                               >  2:77277/1‑149 (MQ=255)                                                       tAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCa                                                                                               >  1:267849/1‑149 (MQ=255)                                                        aaCTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAag                                                                                             <  1:72595/150‑1 (MQ=255)                                                         aCTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAagag                                                                                           <  2:146113/151‑1 (MQ=255)                                                           tAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAgaga                                                                                          >  1:1127177/1‑150 (MQ=255)                                                            aaaGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAgaga                                                                                          <  1:903433/149‑1 (MQ=255)                                                            aaaGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAgaga                                                                                          <  1:978618/149‑1 (MQ=255)                                                             aaGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAAc                                                                                       >  1:528823/1‑151 (MQ=255)                                                              aGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACt                                                                                      >  2:325734/1‑151 (MQ=255)                                                              aGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACt                                                                                      <  2:430035/151‑1 (MQ=255)                                                                   tACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCa                                                                                   <  1:902696/149‑1 (MQ=255)                                                                   tACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCa                                                                                   <  2:949010/149‑1 (MQ=255)                                                                   tACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAAc                                                                                 >  2:302383/1‑151 (MQ=255)                                                                    aCTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAAc                                                                                 <  1:525948/150‑1 (MQ=255)                                                                      tGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCgg                                                                              >  2:211964/1‑151 (MQ=255)                                                                       ggTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGt                                                                             <  2:1083917/151‑1 (MQ=255)                                                                        gTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGt                                                                             >  1:553730/1‑150 (MQ=255)                                                                        gTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGt                                                                             >  1:4682/1‑150 (MQ=255)                                                                           taAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTacac                                                                         >  1:1054499/1‑151 (MQ=255)                                                                                cGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGa                                                                    <  1:1177462/151‑1 (MQ=255)                                                                                  gCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAAc                                                                  <  2:1038716/151‑1 (MQ=255)                                                                                    aTGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACg                                                                 >  2:159037/1‑150 (MQ=255)                                                                                       gAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGAttt                                                             <  2:645216/151‑1 (MQ=255)                                                                                          gTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGa                                                          <  2:839646/151‑1 (MQ=255)                                                                                            cACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGAt                                                         <  1:1092416/150‑1 (MQ=255)                                                                                            cACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGAt                                                         <  2:73135/150‑1 (MQ=255)                                                                                                 ttGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGtt                                                   >  2:606427/1‑151 (MQ=255)                                                                                                     aGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCt                                               >  1:1038762/1‑151 (MQ=255)                                                                                                     aGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCt                                               >  1:983046/1‑151 (MQ=255)                                                                                                      gCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTg                                              <  2:780355/151‑1 (MQ=255)                                                                                                        cAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTc                                            <  2:680368/151‑1 (MQ=255)                                                                                                            tCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCAt                                        >  2:379132/1‑151 (MQ=255)                                                                                                                      cGGCAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTtgtg                                  <  1:12525/147‑1 (MQ=255)                                                                                                                         cAATGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGta                            >  1:1198101/1‑150 (MQ=255)                                                                                                                          aaTGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGtaat                          <  1:179053/151‑1 (MQ=255)                                                                                                                          aaTGTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGtaat                          >  2:359960/1‑151 (MQ=255)                                                                                                                             gTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGtaataa                        <  2:49154/150‑1 (MQ=255)                                                                                                                             gTCTTCGTTCTTCGTATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGtaataa                        <  1:28725/150‑1 (MQ=255)                                                                                                                              tcttcgttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAgg                      >  1:902229/1‑151 (MQ=255)                                                                                                                               cttcgttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAgg                      >  1:79346/1‑150 (MQ=255)                                                                                                                                 tcgttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAgg                      <  1:749675/148‑1 (MQ=255)                                                                                                                                  cgttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGgaga                   >  1:94977/1‑150 (MQ=255)                                                                                                                                   gttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAAc                 <  2:803923/151‑1 (MQ=255)                                                                                                                                    ttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACg                >  1:1191476/1‑151 (MQ=255)                                                                                                                                    ttcttcgtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACg                >  1:852756/1‑151 (MQ=255)                                                                                                                                          gtATCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGc          >  2:827031/1‑151 (MQ=255)                                                                                                                                            aTCAATAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGcc         <  2:490896/150‑1 (MQ=255)                                                                                                                                               aaTAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGCCGGtt     <  2:782514/151‑1 (MQ=255)                                                                                                                                                 tAGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGCCGGTTcc   >  2:508016/1‑151 (MQ=255)                                                                                                                                                  aGTCGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGCCGGTTccc  >  1:586237/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTCCATTATGCGAAATAATGTTGCTAATTTCTTTTTCTATCGGACTGGTATCGATAACTAAAGCCTTACTGGTATAAGCCGGCATGGAGGTCACGGTTGAAGCCAGCTCAGGCATCTCGGCAATGTCTTCGTTCTTCGTATCAATAGTTGCTGTCATCGATAAACCAATACGCAAGGGGTGTTCCATGAGTTCTTTTGGATCAAGAGAAACTTCAACCGGTACACGCTGAACGATTTTGATCCAGTTCCCTGTCGCATTTTGTGCAGGTAATAAGGAGAACGCATTGCCGGTTCCC  >  NC_000913/2482184‑2482479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑