Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,495,245	G→A	S16S (AGC→AGT)	yfdY ←	DUF2545 family putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,495,245	0	G	A	100.0%	317.0 / NA	82	S16S (AGC→AGT)	yfdY	DUF2545 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/44);  total (38/44)

CCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATG  >  NC_000913/2495097‑2495388                                                                                                                                                     |                                                                                                                                                ccGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACt                                                                                                                                               >  2:998458/1‑151 (MQ=255)   ggggCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACtt                                                                                                                                              >  2:660463/1‑150 (MQ=255)        aaGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGc                                                                                                                                          >  2:648540/1‑149 (MQ=255)          aaaaCAAAAATATCCCCGTCACTATTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGAc                                                                                                                                      <  1:326623/150‑1 (MQ=255)           aaaCATAAATACCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGAc                                                                                                                                      <  2:123251/150‑1 (MQ=255)            aaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACa                                                                                                                                     >  2:590167/1‑150 (MQ=255)            aaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACa                                                                                                                                     >  1:57176/1‑150 (MQ=255)               aaaaaTATCCCCGTCACTAGTTAATTACCGCCAGTTACCCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATa                                                                                                                                  <  2:1088437/148‑1 (MQ=255)                tAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATac                                                                                                                                 >  2:126985/1‑150 (MQ=255)                 aaaTATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGATGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATacac                                                                                                                               >  1:245264/1‑151 (MQ=255)                  agagccccgttcgcaaGTACATGACCGCAAGTTAACAACATGGGGAAGTAAAAAATGTGTCCAGCGAGCACCTTCTCGCTGAAGAATGAAGACACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATacac                                                                                                                               <  1:1003577/135‑1 (MQ=255)                   atatCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCCCCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAg                                                                                                                             <  2:314229/151‑1 (MQ=255)                     atCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAggg                                                                                                                           >  1:686114/1‑151 (MQ=255)                     acccccGTCACTAGTTCATTCCCGCCAGTAAACCACATGGTGAAGTAAAAAAGGAGTGCAGCGAGGTCCCCCATGCCGAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAggg                                                                                                                           <  1:436248/149‑1 (MQ=255)                       ccccGTCCCTATTTAATTACCGCCAGTTACCCACATGGTAAAGTAATAAATGAGTGCATCGAGATTCCCCATGCCAAAAAAAGAAGAAACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAggg                                                                                                                           <  1:968457/149‑1 (MQ=255)                        cccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCg                                                                                                                         >  2:62762/1‑150 (MQ=255)                         ccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCg                                                                                                                         >  2:1183106/1‑149 (MQ=255)                             cACTATTTCATTCCCGCCAGTTAACCACAGGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGaaa                                                                                                                    <  1:1199850/150‑1 (MQ=255)                             cACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAAc                                                                                                                   >  1:176446/1‑151 (MQ=255)                              aCTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAAc                                                                                                                   >  2:544371/1‑150 (MQ=255)                                tAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATc                                                                                                                >  2:1172800/1‑151 (MQ=255)                                 aGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAAAATcc                                                                                                               >  1:1020584/1‑151 (MQ=255)                                   ttCATTCCCGCCAGTTACCCACATGGTGAAGTAATAAAGGAGTGCAGCGAGGACCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATc                                                                                                                <  2:299765/148‑1 (MQ=255)                                    tCATTCCCGCCAGTTAACCACATGGTGAAGAAATAAATGAGTGCAGCGAGGAACCCCAGGCCAAAAAAAGAAGAGTCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCAt                                                                                                             <  1:943737/150‑1 (MQ=255)                                      aTTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATaa                                                                                                           >  2:1031331/1‑150 (MQ=255)                                       ttACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATaa                                                                                                           <  2:495718/149‑1 (MQ=255)                                        tcccGCCAGTTAACCACATGGTGAAGTAAAAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATaa                                                                                                           <  1:338783/146‑1 (MQ=255)                                          ccGCCATTTAACCACATGGTAAATTAAGAAATGAGTGCGGCGAGTATCACCATGCGGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAAt                                                                                                         <  1:415020/148‑1 (MQ=255)                                          ccGCCAGTTACCCACAGGGTAAAGTAATAAATGAGTGAAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAAtt                                                                                                        <  1:594440/149‑1 (MQ=255)                                          ccGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGACCACCATGCCGAAGAAAAAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAAt                                                                                                         <  2:685558/148‑1 (MQ=255)                                               aGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATa                                                                                                 <  2:282597/151‑1 (MQ=255)                                               aGTTAACCACATGGTGAAGTAAAAAATGAGTGCAGCGAGGACCCCCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATa                                                                                                 <  2:458541/151‑1 (MQ=255)                                                 ttAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGc                                                                                               >  2:990094/1‑151 (MQ=255)                                                   acccACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGc                                                                                               <  2:429873/147‑1 (MQ=255)                                                       acaTGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcc                                                                                         >  1:1127340/1‑151 (MQ=255)                                                        caTGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcct                                                                                        >  2:253285/1‑151 (MQ=255)                                                        aaggttGAAGTAATAAACGAGACCGGCGGGGATCCCTATGCCAAAGAAGGAAGCGACCGCCGATACCACGTTCAGTACCTGACCGATGTGGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCGCa                                                                                            <  1:234969/142‑1 (MQ=255)                                                         aTGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcctc                                                                                       >  1:221869/1‑151 (MQ=255)                                                           ggTGAAGTAATAAATGATTGCACGGAGAATCCCAAGGCCAAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcctcc                                                                                      <  1:671314/150‑1 (MQ=255)                                                           ggTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAAAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcctc                                                                                       <  2:221872/149‑1 (MQ=255)                                                           ggTGAAGAAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAAAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAcctcct                                                                                     <  2:57176/151‑1 (MQ=255)                                                            gTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTg                                                                                    >  1:484698/1‑151 (MQ=255)                                                              gAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGcc                                                                                  >  2:427949/1‑151 (MQ=255)                                                               aaataaaaaATGAGTGCAGGGGGGATCCCCATGCAGAAAAAAAAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGcc                                                                                  <  2:1095740/147‑1 (MQ=255)                                                                    ataaATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCt                                                                             >  2:665940/1‑150 (MQ=255)                                                                    ataaATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCt                                                                             >  2:850489/1‑150 (MQ=255)                                                                        aTGAGTGCAGCGAGGATCCCCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTaaaaa                                                                        <  2:909067/151‑1 (MQ=255)                                                                            gTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAAcc                                                                     <  2:818692/150‑1 (MQ=255)                                                                               cAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGt                                                                  >  2:335942/1‑150 (MQ=255)                                                                                aGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTa                                                                 >  1:97051/1‑150 (MQ=255)                                                                                aGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTa                                                                 >  1:359585/1‑150 (MQ=255)                                                                                   gAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACtt                                                             >  2:116422/1‑151 (MQ=255)                                                                                     ggATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTc                                                            >  2:366819/1‑150 (MQ=255)                                                                                        tCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTc                                                        >  1:115269/1‑151 (MQ=255)                                                                                          aCCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCAc                                                      >  1:54291/1‑151 (MQ=255)                                                                                          aCCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCAc                                                      >  2:1157997/1‑151 (MQ=255)                                                                                              tGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACttt                                                   >  1:970636/1‑150 (MQ=255)                                                                                                 cGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAAt                                                <  1:589845/150‑1 (MQ=255)                                                                                                  gaagaaAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAAt                                                >  2:1154631/1‑149 (MQ=255)                                                                                                   aaaaaagaaaagaCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATa                                               <  1:233175/146‑1 (MQ=255)                                                                                                   aaaaaagaaGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAg                                              <  1:442301/147‑1 (MQ=255)                                                                                                     aaaagaaGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTaa                                           <  1:248884/150‑1 (MQ=255)                                                                                                      aaaaaaGAGTCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTa                                            <  1:382558/149‑1 (MQ=255)                                                                                                              gaCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCt                                  >  1:886098/1‑151 (MQ=255)                                                                                                               aCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAAt                                     <  1:351688/147‑1 (MQ=255)                                                                                                                ccgccgATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCt                                  <  1:369042/149‑1 (MQ=255)                                                                                                                 cgccgATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTaa                               <  1:1049530/151‑1 (MQ=255)                                                                                                                  gccgATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAAc                              <  1:551410/151‑1 (MQ=255)                                                                                                                   ccgACACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAAcc                             >  1:280335/1‑151 (MQ=255)                                                                                                                    cgATACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACct                            <  1:990509/151‑1 (MQ=255)                                                                                                                       tACCACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTg                         >  2:1190152/1‑151 (MQ=255)                                                                                                                        aCCACGTTCAGGACCTGACCGATGGAGACACGTACGCAGACAATACAAAGGGCGAGAAACATCCATAAATTAATCATAACTCAACCTCCTGCCGCGCTAAAAAACCTGTAAATTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTgg                        >  1:748466/1‑151 (MQ=255)                                                                                                                         ccACGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGc                       <  2:153554/151‑1 (MQ=255)                                                                                                                         ccACGTGCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCGCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGc                       <  1:1103295/151‑1 (MQ=255)                                                                                                                            cGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAAt                    <  1:253282/151‑1 (MQ=255)                                                                                                                            cGTTCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAAt                    <  1:32863/151‑1 (MQ=255)                                                                                                                              ttCAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAGCCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCGCTTTAATAGTAAGACAATCCTTAACCTCTGGCAAtt                   <  1:745526/150‑1 (MQ=255)                                                                                                                                cAGTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAAtttt                 <  1:801337/150‑1 (MQ=255)                                                                                                                                  gTACCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGc              <  1:264241/151‑1 (MQ=255)                                                                                                                                   tcccTAACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCCCCTCCCGCGCTAAAAAACCTGTAACTTCAGTCACTGTAAGAGTAAGAAAGTCCGTAACCTCTGGCAAtttt                 <  1:731203/145‑1 (MQ=255)                                                                                                                                    aCCTGACCGATGTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCt             <  2:962843/150‑1 (MQ=255)                                                                                                                                               gTAGCCACTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATg  >  1:358578/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATG  >  NC_000913/2495097‑2495388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑