Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,548,346	G→A	G82D (GGC→GAC)	yfeW →	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,548,346	0	G	A	100.0%	322.4 / NA	81	G82D (GGC→GAC)	yfeW	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/37);  total (44/37)

GCAGGGTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTGCGAAAT  >  NC_000913/2548198‑2548492                                                                                                                                                     |                                                                                                                                                   gCAGGGTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACa                                                                                                                                                  <  2:50454/151‑1 (MQ=255)  cAGGGTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAg                                                                                                                                                 <  1:1093798/151‑1 (MQ=255)   aGGGTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGc                                                                                                                                                >  1:867252/1‑151 (MQ=255)     ggTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCgt                                                                                                                                              <  2:877645/151‑1 (MQ=255)         tAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGtt                                                                                                                                           <  2:396051/150‑1 (MQ=255)           aCGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGAt                                                                                                                                        <  2:847315/151‑1 (MQ=255)           aCGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGAt                                                                                                                                        <  1:623882/151‑1 (MQ=255)             gTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATgg                                                                                                                                      >  2:268925/1‑151 (MQ=255)                 aaCGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAAc                                                                                                                                   >  1:1066263/1‑150 (MQ=255)                      cTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTg                                                                                                                             >  2:853471/1‑151 (MQ=255)                        tAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTc                                                                                                                           >  1:590319/1‑151 (MQ=255)                          aCCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCaa                                                                                                                         <  2:784410/151‑1 (MQ=255)                                tGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGccac                                                                                                                   >  1:606815/1‑151 (MQ=255)                                   aTCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCcaccac                                                                                                                <  2:490349/151‑1 (MQ=255)                                   aTCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCcaccac                                                                                                                <  2:823860/151‑1 (MQ=255)                                       cTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCggg                                                                                                            >  1:945364/1‑151 (MQ=255)                                          gATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACg                                                                                                         >  2:606438/1‑151 (MQ=255)                                             tAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCt                                                                                                       <  1:1158325/150‑1 (MQ=255)                                             tAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTg                                                                                                      >  1:685485/1‑151 (MQ=255)                                              aGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGt                                                                                                     <  2:254142/151‑1 (MQ=255)                                              aGCCAGCAAGTTGATGTCGGTTATCCAAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGAAGCTg                                                                                                      >  1:242706/1‑150 (MQ=255)                                               gCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGt                                                                                                     >  1:523743/1‑150 (MQ=255)                                               gCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTa                                                                                                    <  1:640196/151‑1 (MQ=255)                                                   gCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGAc                                                                                                <  2:113169/151‑1 (MQ=255)                                                    cAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGAcc                                                                                               <  2:968904/151‑1 (MQ=255)                                                     aaGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCt                                                                                              <  2:506895/151‑1 (MQ=255)                                                           aTGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTc                                                                                        <  1:558402/151‑1 (MQ=255)                                                             gTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCaa                                                                                      <  1:987517/151‑1 (MQ=255)                                                             gTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCa                                                                                       >  2:788348/1‑150 (MQ=255)                                                               cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCaaa                                                                                     <  2:740671/150‑1 (MQ=255)                                                               cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAac                                                                                    <  2:1088630/151‑1 (MQ=255)                                                                ggTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAaca                                                                                   <  1:863457/151‑1 (MQ=255)                                                                  ttATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACAcc                                                                                 >  2:1094110/1‑151 (MQ=255)                                                                    aTCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCa                                                                                >  2:953709/1‑150 (MQ=255)                                                                    aTCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCa                                                                                >  1:956426/1‑150 (MQ=255)                                                                          gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATg                                                                           <  1:998077/149‑1 (MQ=255)                                                                          gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGt                                                                          >  2:822233/1‑150 (MQ=255)                                                                          gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTa                                                                         <  1:817367/151‑1 (MQ=255)                                                                                  ctgctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACg                                                                  >  2:753657/1‑150 (MQ=255)                                                                                    gctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAAc                                                               <  1:1050730/151‑1 (MQ=255)                                                                                        aTCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCg                                                           >  2:651093/1‑151 (MQ=255)                                                                                         tCATTAAAGATAGTCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCg                                                           >  1:741656/1‑150 (MQ=255)                                                                                         tCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGc                                                          >  1:935103/1‑151 (MQ=255)                                                                                         tCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGc                                                          >  1:672957/1‑151 (MQ=255)                                                                                           aTTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGccc                                                        >  2:332366/1‑151 (MQ=255)                                                                                            ttAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCt                                                       >  2:1136911/1‑151 (MQ=255)                                                                                            ttAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCt                                                       >  2:506449/1‑151 (MQ=255)                                                                                             tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCt                                                       <  2:617354/150‑1 (MQ=255)                                                                                             tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTg                                                      >  1:372149/1‑151 (MQ=255)                                                                                              aaaGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGc                                                     <  1:1173982/151‑1 (MQ=255)                                                                                               aaGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGc                                                     >  1:206885/1‑150 (MQ=255)                                                                                               aaGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGc                                                     >  1:552080/1‑150 (MQ=255)                                                                                               aaGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGc                                                     >  2:531621/1‑150 (MQ=255)                                                                                                  aTAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaaa                                                 <  1:518173/151‑1 (MQ=255)                                                                                                  aTAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaaa                                                 <  2:558882/151‑1 (MQ=255)                                                                                                  aTAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaa                                                  >  1:1108090/1‑150 (MQ=255)                                                                                                     aTCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCt                                              <  2:328897/151‑1 (MQ=255)                                                                                                       cAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtg                                             >  1:24380/1‑150 (MQ=255)                                                                                                        aGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgat                                           >  2:739073/1‑151 (MQ=255)                                                                                                         gATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatg                                          >  1:6711/1‑151 (MQ=255)                                                                                                         gATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatg                                          >  1:728613/1‑151 (MQ=255)                                                                                                         gATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatg                                          <  2:297233/151‑1 (MQ=255)                                                                                                          aTTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatga                                         <  1:750999/151‑1 (MQ=255)                                                                                                           ttGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatga                                         <  1:56956/150‑1 (MQ=255)                                                                                                                     cAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCaa                               >  2:1129040/1‑150 (MQ=255)                                                                                                                      aaaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAAc                             >  2:306325/1‑151 (MQ=255)                                                                                                                       aaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACt                            >  1:1131747/1‑151 (MQ=255)                                                                                                                          ccTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGc                          >  1:866879/1‑150 (MQ=255)                                                                                                                               ggAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTg                    <  1:973905/151‑1 (MQ=255)                                                                                                                                 aGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGAc                  >  1:528971/1‑151 (MQ=255)                                                                                                                                 aGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGAc                  >  1:656037/1‑151 (MQ=255)                                                                                                                                    ggcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGAt               <  1:982377/151‑1 (MQ=255)                                                                                                                                      cgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCg             <  1:820424/151‑1 (MQ=255)                                                                                                                                      cgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCg             >  2:219564/1‑151 (MQ=255)                                                                                                                                      cgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCg             <  2:928452/151‑1 (MQ=255)                                                                                                                                       gAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCgg            >  2:182227/1‑151 (MQ=255)                                                                                                                                        aaaaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCgg            >  1:146835/1‑150 (MQ=255)                                                                                                                                        aaaaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCgg            >  1:371359/1‑150 (MQ=255)                                                                                                                                              tACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTGCg      >  2:381419/1‑150 (MQ=255)                                                                                                                                                 gATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTGCGAAat  <  2:411933/151‑1 (MQ=255)                                                                                                                                                 gATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTGCGAAat  <  2:475457/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCAGGGTTTAACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTGCGAAAT  >  NC_000913/2548198‑2548492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑