Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,902	C→T	P210P (CCG→CCA)	iscS ←	cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,902	0	C	T	100.0%	287.0 / NA	70	P210P (CCG→CCA)	iscS	cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/31);  total (39/31)

TTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGAT  >  NC_000913/2660754‑2661045                                                                                                                                                     |                                                                                                                                                ttGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTgg                                                                                                                                               >  2:872301/1‑151 (MQ=255)   gcgatgcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTggg                                                                                                                                              <  2:639787/150‑1 (MQ=255)     gatgcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCa                                                                                                                                           <  1:768757/151‑1 (MQ=255)       tgcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCAt                                                                                                                                          >  2:1159096/1‑150 (MQ=255)        gcgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAg                                                                                                                                        <  1:414455/151‑1 (MQ=255)         cgatAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCAt                                                                                                                                          >  2:871132/1‑148 (MQ=255)            tAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGAttt                                                                                                                                    >  2:51172/1‑151 (MQ=255)             aGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGAtttt                                                                                                                                   >  2:710653/1‑151 (MQ=255)             aGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGAtttt                                                                                                                                   >  1:186358/1‑151 (MQ=255)              ggCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTtg                                                                                                                                  >  2:466216/1‑151 (MQ=255)              ggCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTtg                                                                                                                                  <  1:1048333/151‑1 (MQ=255)                 cTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTtgtg                                                                                                                                >  1:485182/1‑150 (MQ=255)                   cGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGAcc                                                                                                                             <  1:893392/151‑1 (MQ=255)                   cGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTGTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATCGCTTTTGTGAc                                                                                                                              >  1:108298/1‑150 (MQ=255)                     cccATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCgg                                                                                                                           <  2:565318/151‑1 (MQ=255)                       cATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCgg                                                                                                                           >  2:914455/1‑149 (MQ=255)                        aTTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCgg                                                                                                                           >  1:525568/1‑148 (MQ=255)                        aTTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCgg                                                                                                                           >  1:861608/1‑148 (MQ=255)                          tCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGaa                                                                                                                       >  2:1107614/1‑150 (MQ=255)                            cgacgaTCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGa                                                                                                                    <  1:526330/151‑1 (MQ=255)                                gaTCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATc                                                                                                                >  1:453269/1‑151 (MQ=255)                                     ggTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGt                                                                                                            <  2:216655/150‑1 (MQ=255)                                        gAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCaa                                                                                                         <  1:73468/150‑1 (MQ=255)                                          aCAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACtt                                                                                                      <  2:823374/151‑1 (MQ=255)                                                agagTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACt                                                                                                >  2:197844/1‑151 (MQ=255)                                                 gagTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTg                                                                                               >  1:553839/1‑151 (MQ=255)                                                     gCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTc                                                                                           >  2:1131265/1‑151 (MQ=255)                                                               aTACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAg                                                                                 <  2:718245/151‑1 (MQ=255)                                                                   cgcgCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGc                                                                               >  1:765994/1‑149 (MQ=255)                                                                    gcgcTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGt                                                                            >  1:198166/1‑151 (MQ=255)                                                                         cGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTa                                                                         <  2:449452/149‑1 (MQ=255)                                                                                 ccgccgTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGctct                                                               >  1:783397/1‑151 (MQ=255)                                                                                    ccgTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTgtg                                                            <  2:1167093/151‑3 (MQ=255)                                                                                      gTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGtt                                                          <  2:924444/151‑1 (MQ=255)                                                                                        gCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGc                                                        <  1:831099/151‑1 (MQ=255)                                                                                        gCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGc                                                        >  1:177822/1‑151 (MQ=255)                                                                                          aTTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCAt                                                      >  2:787264/1‑151 (MQ=255)                                                                                              gcgcTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAAc                                                  >  1:906605/1‑151 (MQ=255)                                                                                               cgcTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACg                                                 <  1:381141/151‑1 (MQ=255)                                                                                               cgcTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACg                                                 >  1:493579/1‑151 (MQ=255)                                                                                                gcTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGt                                                <  1:953318/151‑1 (MQ=255)                                                                                                  ttCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTg                                               >  2:125275/1‑150 (MQ=255)                                                                                                    cGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgata                                            <  1:694967/151‑1 (MQ=255)                                                                                                    cGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgat                                             <  2:908597/150‑1 (MQ=255)                                                                                                     gATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgat                                             <  1:990209/149‑1 (MQ=255)                                                                                                        gCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgatagata                                        <  2:655077/151‑1 (MQ=255)                                                                                                        gCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgatagat                                         <  1:1131237/150‑1 (MQ=255)                                                                                                        gCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTgatagat                                         >  1:467304/1‑150 (MQ=255)                                                                                                          gTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAAt                                      <  1:478214/151‑1 (MQ=255)                                                                                                              gcgcgGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCa                                  <  1:798530/151‑1 (MQ=255)                                                                                                              gcgcgGTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCa                                  <  1:992274/151‑1 (MQ=255)                                                                                                                  ggTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACg                                >  1:972674/1‑149 (MQ=255)                                                                                                                   gTTTACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCACCAACGTGATAGATAATGCCACGAGc                             >  1:754151/1‑151 (MQ=255)                                                                                                                      tACGACGTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACg                          >  1:1020104/1‑151 (MQ=255)                                                                                                                         gacgTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGc                        >  2:621364/1‑150 (MQ=255)                                                                                                                          acgTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGcac                      >  1:584254/1‑151 (MQ=255)                                                                                                                           cgTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGcac                      >  1:1096168/1‑150 (MQ=255)                                                                                                                            gTACATACAGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACAt                    <  2:1172856/151‑1 (MQ=255)                                                                                                                               catacaGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTc                 >  1:560638/1‑151 (MQ=255)                                                                                                                                    aGCGCACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCg             >  2:609505/1‑150 (MQ=255)                                                                                                                                     gcgcACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGa            >  1:1005810/1‑150 (MQ=255)                                                                                                                                      cgcACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGAt           >  1:977464/1‑150 (MQ=255)                                                                                                                                      cgcACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATa          <  1:111327/151‑1 (MQ=255)                                                                                                                                       gcACCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAg         <  1:108537/151‑1 (MQ=255)                                                                                                                                         aCCGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGATTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGcc       >  1:191401/1‑151 (MQ=255)                                                                                                                                          ccGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcg      <  1:918003/151‑1 (MQ=255)                                                                                                                                           cGATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgc     <  2:526784/151‑1 (MQ=255)                                                                                                                                            gATACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCcgcg    >  1:729412/1‑151 (MQ=255)                                                                                                                                             aTACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGa   <  2:101380/151‑1 (MQ=255)                                                                                                                                              tACCTTTTGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGat  >  1:168551/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                TTGCGATGCGATAGGCCTCGCCCATTCCGACGATCTGGTGAACAGGCAGAGTGCCGGAACGCATACCGCGCTCGTGACCGCCGCCGTGCATTTGCGCTTCGATGCGTACGCGCGGTTTACGACGTACATACAGCGCACCGATACCTTTCGGGCCATAGATTTTGTGACCGGAGAAAGACATCAGGTCAACTTTCAACTGGCTCAGGTCGATAGGCAGTTTACCCACGCTCTGGGTTGCATCAACGTGATAGATAATGCCACGAGCACGGCACATTTCGCCGATAGCCGCGAT  >  NC_000913/2660754‑2661045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑