Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,674,912	T→C	S284G (AGC→GGC)	yphC ←	putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,674,912	0	T	C	100.0%	204.8 / NA	57	S284G (AGC→GGC)	yphC	putative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

CCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAAT  >  NC_000913/2674768‑2675059                                                                                                                                                 |                                                                                                                                                    cctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTc                                                                                                                                               <  1:60428/151‑1 (MQ=255) cctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTc                                                                                                                                               <  1:956197/151‑1 (MQ=255) cctgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTc                                                                                                                                               <  1:477883/151‑1 (MQ=255)   tgTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCg                                                                                                                                              >  2:890341/1‑150 (MQ=255)    gTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCg                                                                                                                                              <  1:1022997/149‑1 (MQ=255)      tCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAAtt                                                                                                                                          >  1:850356/1‑151 (MQ=255)       ccAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAAtt                                                                                                                                          <  1:674751/150‑1 (MQ=255)       ccAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAAtt                                                                                                                                          <  1:536456/150‑1 (MQ=255)       ccAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTc                                                                                                                                         >  2:1159973/1‑151 (MQ=255)        cAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTcc                                                                                                                                        >  2:168827/1‑151 (MQ=255)             agaAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACttt                                                                                                                                   <  2:314441/151‑1 (MQ=255)                aGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTcc                                                                                                                                <  1:95350/151‑1 (MQ=255)                  cGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCgg                                                                                                                              >  1:666089/1‑151 (MQ=255)                  cGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCgg                                                                                                                              <  2:1091788/151‑1 (MQ=255)                    aTGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGtt                                                                                                                            >  1:575122/1‑151 (MQ=255)                      gggTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGttt                                                                                                                           <  1:963735/150‑1 (MQ=255)                       ggTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTc                                                                                                                          >  2:57556/1‑150 (MQ=255)                       ggTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCa                                                                                                                         <  1:181282/151‑1 (MQ=255)                          aaTGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCa                                                                                                                      <  1:829762/151‑1 (MQ=255)                             ggCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATg                                                                                                                   >  1:622828/1‑151 (MQ=255)                                 ttACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTaa                                                                                                                >  2:696748/1‑150 (MQ=255)                                  tACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAac                                                                                                              >  1:850811/1‑151 (MQ=255)                                   aCGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAacc                                                                                                             <  1:1016956/151‑1 (MQ=255)                                       ggCCACAGCTTCCAGTACGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGCCCTCGAATTCCACTTTTCCGGTTTCACCAATTTAAACCAccc                                                                                                         >  1:836991/1‑151 (MQ=255)                                           acaGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTcc                                                                                                     <  2:153269/151‑1 (MQ=255)                                                 tCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGt                                                                                                <  1:192077/150‑1 (MQ=255)                                                  ccAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCa                                                                                              <  1:696738/151‑1 (MQ=255)                                                    aGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGc                                                                                            <  2:702027/151‑1 (MQ=255)                                                       ccGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATTGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTGTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCg                                                                                           <  1:40200/149‑1 (MQ=255)                                                              aTCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGc                                                                                  >  2:315806/1‑151 (MQ=255)                                                               tCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGc                                                                                  >  1:317649/1‑150 (MQ=255)                                                                   gggCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTg                                                                              <  1:431440/150‑1 (MQ=255)                                                                         aTTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCaa                                                                       <  1:611357/151‑1 (MQ=255)                                                                            tttCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGc                                                                     <  1:709212/150‑1 (MQ=255)                                                                            tttCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGc                                                                     <  1:537424/150‑1 (MQ=255)                                                                             ttCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCg                                                                    >  2:611652/1‑150 (MQ=255)                                                                               ccATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGAcc                                                                 >  2:178610/1‑151 (MQ=255)                                                                               ccATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGAcc                                                                 >  1:831364/1‑151 (MQ=255)                                                                                   atGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCg                                                             <  2:163027/151‑1 (MQ=255)                                                                                         cAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCAtt                                                        >  2:548121/1‑150 (MQ=255)                                                                                           gACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTAcc                                                     <  1:892532/151‑1 (MQ=255)                                                                                               gggccaccAGGAGCCGATAATCCGCCGTTGATGGGGCATCAGATCGGCGGCGACCCCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGa                                                  <  1:876657/144‑1 (MQ=255)                                                                                                gTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAAc                                                >  2:154560/1‑151 (MQ=255)                                                                                                gTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAAc                                                <  2:224802/151‑1 (MQ=255)                                                                                                       aGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAg                                         <  1:333705/151‑1 (MQ=255)                                                                                                        ggAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAgc                                        >  1:1090357/1‑151 (MQ=255)                                                                                                           gCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAgcgc                                      >  2:188480/1‑150 (MQ=255)                                                                                                               aTAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACAt                                 <  1:571824/151‑1 (MQ=255)                                                                                                                tAATCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGT‑AACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATcc                               >  1:303544/1‑151 (MQ=255)                                                                                                                   tCCGCCGTTGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCcgc                             >  2:137723/1‑151 (MQ=255)                                                                                                                           tGATGGTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGt                     >  1:657855/1‑151 (MQ=255)                                                                                                                               ggTGCATCAGATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGt                 >  2:913702/1‑151 (MQ=255)                                                                                                                                        gATCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGc        <  2:750503/151‑1 (MQ=255)                                                                                                                                          tCGGCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCg       >  1:49196/1‑150 (MQ=255)                                                                                                                                            ggCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGAt     >  1:992976/1‑150 (MQ=255)                                                                                                                                            ggCGCCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATa    <  1:755392/151‑1 (MQ=255)                                                                                                                                              cgcCGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAAt  >  1:158530/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCTGTTCCAGCGAGAAGCGATGGGTAATGGCGTTACGCGGCCACAGCTTCCAGTCCGTCAGATCATGGGCGCATTTTTCCATATGGAACAGACTGGTCACCCAGGAGCCGATAATCCGCCGTTGATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAAT  >  NC_000913/2674768‑2675059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑