Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,291	G→A	G812G (GGC→GGT)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,291	0	G	A	100.0%	292.6 / NA	76	G812G (GGC→GGT)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/40);  total (36/40)

ATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAC  >  NC_000913/2780144‑2780435                                                                                                                                                     |                                                                                                                                                 aTTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTAcc                                                                                                                                                <  2:110230/150‑1 (MQ=255)   tATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGt                                                                                                                                             >  1:822778/1‑151 (MQ=255)        ttGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGc                                                                                                                                          >  2:728330/1‑149 (MQ=255)        ttGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCa                                                                                                                                         <  2:118759/150‑1 (MQ=255)          gACTGTCAGCTTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTAAAACTGTTTGTATT‑CCGCCGTCGTTAATTTTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAg                                                                                                                                        <  1:896425/149‑1 (MQ=255)              gTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCCCCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCt                                                                                                                                   <  1:726137/150‑1 (MQ=255)              gTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCtt                                                                                                                                  >  1:961247/1‑151 (MQ=255)              gTCAGCGTACCGCCTTAATTGATCTTTGTTTTTATTGCCTCACCGTAAGCGGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGCCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTgg                                                                                                                                     <  1:600477/148‑1 (MQ=255)               tCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttt                                                                                                                                 >  2:902435/1‑151 (MQ=255)                cAGCGTACCGCTTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTTTTGTCTCATTCGCCCCCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCtttt                                                                                                                                <  1:749240/151‑1 (MQ=255)                   cGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgt                                                                                                                              <  2:465061/150‑1 (MQ=255)                   cGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgtt                                                                                                                             >  2:1147034/1‑151 (MQ=255)                    gTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgtt                                                                                                                             <  1:176272/150‑1 (MQ=255)                      aCCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgtttt                                                                                                                           >  1:1025584/1‑150 (MQ=255)                       ccGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTTTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACATTTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgttttg                                                                                                                          <  1:167602/150‑1 (MQ=255)                            ttCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtc                                                                                                                    >  1:21466/1‑151 (MQ=255)                            ttCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtc                                                                                                                    >  2:737161/1‑151 (MQ=255)                            ttCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtc                                                                                                                    >  1:366849/1‑151 (MQ=255)                             tCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtct                                                                                                                   >  2:106064/1‑151 (MQ=255)                                ttGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTccc                                                                                                                >  1:1152002/1‑151 (MQ=255)                                 tGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTTTT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTccc                                                                                                                <  1:960174/150‑1 (MQ=255)                                      tttGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGc                                                                                                          >  2:123779/1‑151 (MQ=255)                                          tttttATTGCCTCTCTGTTAGCGGAAACGGTTTGTATT‑CCGCCGTCGTTATTTGTTGTCTCATTCGCCACCCCCTCGACATTTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGccc                                                                                                        <  1:1114082/149‑1 (MQ=255)                                          tttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGccc                                                                                                        <  1:683128/149‑1 (MQ=255)                                           ttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCat                                                                                                      <  1:1084553/150‑1 (MQ=255)                                             ttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATAtt                                                                                                   >  2:28238/1‑151 (MQ=255)                                              tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATAtt                                                                                                   <  2:700203/150‑1 (MQ=255)                                                ttGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGa                                                                                                 <  1:912602/150‑1 (MQ=255)                                                    cctcccctttgcctaaaCTTTTTTTTTTCCCGCCGTCTTAAATTGTTGTCCCATCCCCCCCCCCCCCGACATTTTTTTCCCCGCCGGTGAGCGTCGTACCTGTCGCAGTGGC‑TTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCtg                                                                                             <  1:308194/136‑1 (MQ=255)                                                      ctcCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCtgt                                                                                            <  2:4953/149‑1 (MQ=255)                                                      ctcCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTgtgt                                                                                          >  1:466692/1‑151 (MQ=255)                                                       tcCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGtt                                                                                         >  1:356784/1‑151 (MQ=255)                                                             aGCTGAACCTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTc                                                                                    <  1:802263/150‑1 (MQ=255)                                                             aGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTc                                                                                    >  2:1050902/1‑150 (MQ=255)                                                                tGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCaga                                                                                 >  2:114842/1‑150 (MQ=255)                                                                tGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCaga                                                                                 >  2:62118/1‑150 (MQ=255)                                                                  aaaCTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCaga                                                                                 >  1:792701/1‑148 (MQ=255)                                                                   aaCTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAagag                                                                             <  2:802158/151‑1 (MQ=255)                                                                    aCTGTTTGTTTT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAagag                                                                             <  2:22676/150‑1 (MQ=255)                                                                    aCTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAgg                                                                            >  1:544639/1‑151 (MQ=255)                                                                      tGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAgg                                                                            >  1:434604/1‑149 (MQ=255)                                                                       gTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGgtgt                                                                         >  1:1178806/1‑151 (MQ=255)                                                                        tttGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTc                                                                        >  1:414215/1‑151 (MQ=255)                                                                           gTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTg                                                                      >  2:314395/1‑150 (MQ=255)                                                                            tATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGa                                                                     <  1:547437/150‑1 (MQ=255)                                                                             aTT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                   >  2:413475/1‑151 (MQ=255)                                                                             aTT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                   <  2:369963/151‑1 (MQ=255)                                                                                      gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                           >  1:854282/1‑151 (MQ=255)                                                                                      gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                           >  2:828890/1‑151 (MQ=255)                                                                                      gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                           >  1:587203/1‑151 (MQ=255)                                                                                        cgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTaa                                                         <  2:305732/151‑1 (MQ=255)                                                                                         gtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAac                                                        >  1:85248/1‑151 (MQ=255)                                                                                          ttAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTTTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAac                                                        <  1:1193768/150‑1 (MQ=255)                                                                                          ttAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAac                                                        <  2:130645/150‑1 (MQ=255)                                                                                           tAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAacac                                                      >  2:638780/1‑151 (MQ=255)                                                                                            aaTTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAacac                                                      <  2:674479/150‑1 (MQ=255)                                                                                               tgttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccg                                                  >  2:1052804/1‑151 (MQ=255)                                                                                                  tgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACgccgcc                                                >  1:429372/1‑150 (MQ=255)                                                                                                   gtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCa                                               <  2:21466/150‑1 (MQ=255)                                                                                                       cATTCGCCACCCCCTCGCCAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAttt                                            <  1:995220/149‑1 (MQ=255)                                                                                                       cATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAttt                                            <  1:242511/149‑1 (MQ=255)                                                                                                             ccACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagca                                     <  2:295773/150‑1 (MQ=255)                                                                                                             ccACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagca                                     <  2:240681/150‑1 (MQ=255)                                                                                                                  cccTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGata                                <  2:859581/150‑1 (MQ=255)                                                                                                                   ccccGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATAtt                              <  2:878520/148‑1 (MQ=255)                                                                                                                      cGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTg                             <  1:628006/149‑1 (MQ=255)                                                                                                                      cGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                           <  1:321960/151‑1 (MQ=255)                                                                                                                            ttttttCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                      <  1:28323/150‑1 (MQ=255)                                                                                                                                ttCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                 >  2:159116/1‑151 (MQ=255)                                                                                                                                 tCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCa                    <  1:1092399/147‑1 (MQ=255)                                                                                                                                  cACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                 <  2:83994/149‑1 (MQ=255)                                                                                                                                         ggTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGAt         >  1:152459/1‑150 (MQ=255)                                                                                                                                          gTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGc       <  2:587209/151‑1 (MQ=255)                                                                                                                                            gAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTg     >  2:583206/1‑151 (MQ=255)                                                                                                                                               cgtcgtACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAc  <  2:693648/151‑1 (MQ=255)                                                                                                                                               cgtcgtACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAc  >  1:33533/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 ATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATT‑CCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAC  >  NC_000913/2780144‑2780435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑