Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,830,245	G→A	E145K (GAA→AAA)	srlQ →	D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,830,245	0	G	A	100.0%	330.3 / NA	80	E145K (GAA→AAA)	srlQ	D‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/29);  total (51/29)

TCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTC  >  NC_000913/2830099‑2830392                                                                                                                                                   |                                                                                                                                                    tctcTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGc                                                                                                                                                 >  1:239990/1‑151 (MQ=255)   tctTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCt                                                                                                                                               >  1:939510/1‑151 (MQ=255)    ctTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTg                                                                                                                                              >  2:518005/1‑151 (MQ=255)      tACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCt                                                                                                                                               <  2:351582/148‑1 (MQ=255)       aCTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGccc                                                                                                                                           >  1:213952/1‑151 (MQ=255)        cTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCg                                                                                                                                          >  1:369784/1‑151 (MQ=255)                     aaGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTgcgc                                                                                                                             <  2:70252/151‑1 (MQ=255)                      aGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCCCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGcc                                                                                                                            >  2:572835/1‑151 (MQ=255)                        gAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCg                                                                                                                           >  2:226634/1‑150 (MQ=255)                          aCTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGAcc                                                                                                                        >  1:761247/1‑151 (MQ=255)                           cTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGAcc                                                                                                                        >  2:352747/1‑150 (MQ=255)                           cTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCt                                                                                                                       >  2:961547/1‑151 (MQ=255)                           cTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCt                                                                                                                       >  2:440818/1‑151 (MQ=255)                            tGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTc                                                                                                                      >  2:1199754/1‑151 (MQ=255)                                   gATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAcc                                                                                                               >  1:140630/1‑151 (MQ=255)                                      tattCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTc                                                                                                            >  2:125887/1‑151 (MQ=255)                                       attCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTc                                                                                                            >  1:264415/1‑150 (MQ=255)                                         tCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCaa                                                                                                          <  1:966877/150‑1 (MQ=255)                                         tCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCaa                                                                                                          <  1:44521/150‑1 (MQ=255)                                            gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                                                                      >  2:508898/1‑151 (MQ=255)                                            gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                                                                      >  2:887822/1‑151 (MQ=255)                                            gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                                                                      <  2:110174/151‑1 (MQ=255)                                              gTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCt                                                                                                    >  2:291479/1‑151 (MQ=255)                                                  ggAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCtgatg                                                                                                >  1:541779/1‑151 (MQ=255)                                                    aaGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTgatgat                                                                                              >  2:949438/1‑151 (MQ=255)                                                     aGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGatgatg                                                                                             <  1:823415/151‑1 (MQ=255)                                                      gATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATgg                                                                                            >  2:683817/1‑151 (MQ=255)                                                         aaaTCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTg                                                                                         >  1:922447/1‑151 (MQ=255)                                                          aaTCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTg                                                                                         >  2:1148042/1‑150 (MQ=255)                                                          aaTCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTg                                                                                         >  2:340339/1‑150 (MQ=255)                                                                 cgcgCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTg                                                                                 >  1:240283/1‑151 (MQ=255)                                                                   cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGc                                                                               >  2:990304/1‑151 (MQ=255)                                                                   cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGc                                                                               <  2:45913/151‑1 (MQ=255)                                                                      tgctgGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGAt                                                                            >  2:916696/1‑151 (MQ=255)                                                                       gctgGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATg                                                                           <  1:346599/151‑1 (MQ=255)                                                                              aTGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTc                                                                     >  1:1052773/1‑150 (MQ=255)                                                                                gACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCAt                                                                   <  2:989141/150‑1 (MQ=255)                                                                                gACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCAt                                                                   <  1:758295/150‑1 (MQ=255)                                                                                   cGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGc                                                                 >  2:593845/1‑149 (MQ=255)                                                                                      cAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcg                                                            >  1:916244/1‑151 (MQ=255)                                                                                      cAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcg                                                            <  1:221891/151‑1 (MQ=255)                                                                                       aaaCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgc                                                           >  1:679565/1‑151 (MQ=255)                                                                                       aaaCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgc                                                           >  1:794554/1‑151 (MQ=255)                                                                                       aaaCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgc                                                           >  1:560753/1‑151 (MQ=255)                                                                                        aaCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgcg                                                          >  2:397947/1‑151 (MQ=255)                                                                                        aaCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgcg                                                          >  2:177898/1‑151 (MQ=255)                                                                                         aCCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGgcgcgc                                                         <  1:293182/151‑1 (MQ=255)                                                                                           cGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCgg                                                       >  2:165168/1‑151 (MQ=255)                                                                                            gACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGa                                                      <  1:789116/151‑1 (MQ=255)                                                                                              cGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAtt                                                    >  2:305080/1‑151 (MQ=255)                                                                                               gTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAttt                                                   <  1:913886/151‑1 (MQ=255)                                                                                               gTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAttt                                                   <  1:224733/151‑1 (MQ=255)                                                                                               gTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAttt                                                   <  2:662732/151‑1 (MQ=255)                                                                                                 cACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTaa                                                 <  1:417553/151‑1 (MQ=255)                                                                                                    cGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATga                                              <  2:314218/151‑1 (MQ=255)                                                                                                      cTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATga                                              >  2:32988/1‑149 (MQ=255)                                                                                                        gggCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATgaaga                                           <  1:214162/150‑1 (MQ=255)                                                                                                         ggCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGaagaag                                         <  2:73861/151‑1 (MQ=255)                                                                                                          gCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAagaaga                                        <  1:1156629/151‑1 (MQ=255)                                                                                                             tGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGAttt                                     >  2:31825/1‑151 (MQ=255)                                                                                                              ggcggcAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGAtttt                                    <  2:947170/151‑1 (MQ=255)                                                                                                              ggcggcAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGAtttt                                    <  1:32988/151‑1 (MQ=255)                                                                                                              ggcggcAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGAttt                                     <  1:518002/150‑1 (MQ=255)                                                                                                                cggcAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGc                                  >  2:560593/1‑151 (MQ=255)                                                                                                                   cAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCg                               >  2:1106770/1‑151 (MQ=255)                                                                                                                   cAAAAGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCg                               >  2:1014153/1‑151 (MQ=255)                                                                                                                       aGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcc                           >  1:696759/1‑151 (MQ=255)                                                                                                                       aGCGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcc                           >  2:645027/1‑151 (MQ=255)                                                                                                                         cGGTGCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTccc                          >  1:501842/1‑150 (MQ=255)                                                                                                                             gCTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccaccca                     <  1:652519/151‑1 (MQ=255)                                                                                                                              cTGGATATCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTcccaccc                      >  2:47668/1‑149 (MQ=255)                                                                                                                                  atatCTCCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCgg                >  2:234453/1‑151 (MQ=255)                                                                                                                                      ctcCGTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCgggg              >  2:182526/1‑149 (MQ=255)                                                                                                                                          gTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTgg        <  2:1181414/151‑1 (MQ=255)                                                                                                                                          gTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTgg        <  2:873810/151‑1 (MQ=255)                                                                                                                                          gTAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTgg        >  1:159353/1‑151 (MQ=255)                                                                                                                                           tAGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTggg       >  1:451192/1‑151 (MQ=255)                                                                                                                                            aGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGgc      <  1:374976/151‑1 (MQ=255)                                                                                                                                            aGAACGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGgc      <  2:939529/151‑1 (MQ=255)                                                                                                                                               aCGCAAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGgcgc    >  2:637204/1‑150 (MQ=255)                                                                                                                                                cgcaAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctc  <  2:761258/151‑1 (MQ=255)                                                                                                                                                cgcaAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctc  <  1:454870/151‑1 (MQ=255)                                                                                                                                                cgcaAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctc  >  2:577486/1‑151 (MQ=255)                                                                                                                                                cgcaAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctc  <  1:279997/151‑1 (MQ=255)                                                                                                                                                cgcaAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGcgctc  <  1:322911/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTC  >  NC_000913/2830099‑2830392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑