Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,337	(C)6→7	coding (67/3150 nt)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,331	1	.	C	100.0%	159.4 / NA	46	L25V (CTG→GTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/17);  total (29/17)

TTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑CCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTA  >  NC_000913/484183‑484474                                                                                                                                                      |                                                                                                                                                ttATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑cc                                                                                                                                               >  2:337882/1‑151 (MQ=255)  tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑ccc                                                                                                                                              >  2:400027/1‑151 (MQ=255)       aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑ccccccct                                                                                                                                         <  1:429445/151‑3 (MQ=255)            gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCa                                                                                                                                      >  1:808969/1‑150 (MQ=255)             ttCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCCCCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCaa                                                                                                                                     >  2:548353/1‑150 (MQ=255)              tCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAAc                                                                                                                                    >  2:492375/1‑150 (MQ=255)                  atTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACatgat                                                                                                                               >  1:743351/1‑151 (MQ=255)                     cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACAtgatga                                                                                                                             >  1:95229/1‑150 (MQ=255)                     cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATgatgat                                                                                                                            >  1:445663/1‑151 (MQ=255)                              aaCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGAt                                                                                                                   >  1:483784/1‑151 (MQ=255)                                 cTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTCGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTACGGTATTGCTCCACCGGCAGTTTTAGGATCCCCAGCCCCCCCTGCCAACCTGATGGTAATGGCGGTccc                                                                                                                >  2:60992/1‑149 (MQ=255)                                     gtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                            >  2:1060951/1‑151 (MQ=255)                                     gtCACCGTGTCCTGCACTGTTTGCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                            <  2:875109/151‑1 (MQ=255)                                      tCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                            >  2:823225/1‑150 (MQ=255)                                       cACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGc                                                                                                          >  1:659797/1‑151 (MQ=255)                                         ccGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaa                                                                                                        <  1:600745/151‑1 (MQ=255)                                          cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTATGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaa                                                                                                       <  2:779438/151‑1 (MQ=255)                                          cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaa                                                                                                        >  1:779480/1‑150 (MQ=255)                                           gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaa                                                                                                      <  2:834843/151‑1 (MQ=255)                                             gtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAAt                                                                                                    <  1:208631/151‑1 (MQ=255)                                                       gTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcga                                                                                             <  2:233426/148‑1 (MQ=255)                                                               aTCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGaa                                                                                  <  1:166258/151‑1 (MQ=255)                                                                tCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGaa                                                                                  >  1:759641/1‑150 (MQ=255)                                                                     gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                            <  1:191309/151‑1 (MQ=255)                                                                     gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                            <  1:723142/151‑1 (MQ=255)                                                                     gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                            >  2:61752/1‑151 (MQ=255)                                                                       cGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                             >  1:43405/1‑148 (MQ=255)                                                                           gTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTc                                                                      >  2:702860/1‑151 (MQ=255)                                                                             aggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCtt                                                                    >  1:484569/1‑151 (MQ=255)                                                                             aggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCt                                                                     <  1:813821/150‑1 (MQ=255)                                                                                        aTCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTg                                                         <  2:544932/151‑1 (MQ=255)                                                                                        aTCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGGTTGAGGATCGCCCGCCCCCCCTGCCAACATTATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAGTTAGGCATGTCTTAACGGCCCCTg                                                         >  2:123682/1‑151 (MQ=255)                                                                                          cGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGtt                                                       >  2:347844/1‑151 (MQ=255)                                                                                              aCTGCCGGCGGTGCAATCGTAGGATATGGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTtta                                                     <  2:374018/149‑1 (MQ=255)                                                                                                    ggcggTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGa                                             <  1:523201/151‑1 (MQ=255)                                                                                                        gTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGGCTTAACGGCTCCTGTTTACGTTAAGACt                                           >  2:402517/1‑149 (MQ=255)                                                                                                          gCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTgg                                        <  2:547987/150‑1 (MQ=255)                                                                                                           cAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGAc                                      <  1:462928/151‑1 (MQ=255)                                                                                                                           cgcCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCAc                      >  1:896839/1‑151 (MQ=255)                                                                                                                            gcCACCGGCAGTTTGCGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCAcc                     >  1:862922/1‑151 (MQ=255)                                                                                                                            gcCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCAcc                     >  1:109838/1‑151 (MQ=255)                                                                                                                              cACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGc                   >  2:784843/1‑151 (MQ=255)                                                                                                                                ccGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                 >  1:525409/1‑151 (MQ=255)                                                                                                                                 cGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTg                >  1:256551/1‑151 (MQ=255)                                                                                                                                  ggCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGc               <  1:338270/151‑1 (MQ=255)                                                                                                                                         tGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTg         >  2:878353/1‑150 (MQ=255)                                                                                                                                         tGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGc        >  2:541653/1‑151 (MQ=255)                                                                                                                                           aGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTg      >  1:977319/1‑151 (MQ=255)                                                                                                                                                cGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtta  <  2:212477/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                TTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑CCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTA  >  NC_000913/484183‑484474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑