Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,328,783 (G)5→6 coding (1015/1650 nt) yfaQ ← tandem DUF2300 domain protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,328,7781.G97.0% 235.6 / ‑1.1 66coding (1020/1650 nt)yfaQtandem DUF2300 domain protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (35/29);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328635‑2328923
                                                                                                                                                |                                                                                                                                                   
aTCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCgggggggc                                                                                                                                             >  2:742808/1‑149 (MQ=255)
 tCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAggggggcg                                                                                                                                            >  1:585552/1‑148 (MQ=255)
  cGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAggggggcgt                                                                                                                                           >  1:16940/1‑147 (MQ=255)
  cGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAggggggcgt                                                                                                                                           >  1:505712/1‑147 (MQ=255)
        cgcCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACtttt                                                                                                                                     >  2:285190/1‑151 (MQ=255)
         gcCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTc                                                                                                                                    <  1:1027645/151‑1 (MQ=255)
         aaccccGGGGTAGATGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCACGAATGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACtttt                                                                                                                                     >  2:521968/3‑150 (MQ=255)
               gTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTcg                                                                                                                              >  2:462339/1‑151 (MQ=255)
                 ggTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTcgcg                                                                                                                            <  2:810862/151‑1 (MQ=255)
                 ggTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTcgcg                                                                                                                            >  1:429657/1‑151 (MQ=255)
                   tCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGAt                                                                                                                          >  1:237700/1‑151 (MQ=255)
                     gCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATcc                                                                                                                        >  2:26152/1‑151 (MQ=255)
                        ggCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGTGGGCGTACTTTTCGCCTCGCGATCCAg                                                                                                                      <  2:820436/150‑1 (MQ=255)
                          cGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAAc                                                                                                                   <  2:156565/151‑1 (MQ=255)
                           gAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAAct                                                                                                                  >  1:615604/1‑151 (MQ=255)
                            aaGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAActc                                                                                                                 >  1:893422/1‑151 (MQ=255)
                             aGCAGAAACACGCTGCGTGTCGCTGCTGTCGGGGATGGTCAAGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTAGCCTCGCGATCCAGAtctcg                                                                                                                <  2:808462/151‑2 (MQ=255)
                             aGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAActct                                                                                                                <  1:604637/151‑1 (MQ=255)
                              gCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTg                                                                                                               <  2:356595/151‑1 (MQ=255)
                              gCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGAAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTg                                                                                                               >  1:480362/1‑151 (MQ=255)
                                     cacGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGt                                                                                                        >  2:645819/1‑151 (MQ=255)
                                      acGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGt                                                                                                        >  2:852042/1‑150 (MQ=255)
                                         cTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTActc                                                                                                    >  1:341446/1‑151 (MQ=255)
                                          tGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTActct                                                                                                   >  1:542410/1‑151 (MQ=255)
                                              tGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCAc                                                                                               >  2:905549/1‑151 (MQ=255)
                                                gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                                                                                             <  1:491723/151‑1 (MQ=255)
                                                gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                                                                                             <  2:588215/151‑1 (MQ=255)
                                                        gTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGAt                                                                                      <  1:440750/150‑1 (MQ=255)
                                                                tGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGc                                                                              >  1:381057/1‑150 (MQ=255)
                                                                         aaTCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGtt                                                                    >  2:836841/1‑151 (MQ=255)
                                                                            cGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTg                                                                  <  2:832173/150‑1 (MQ=255)
                                                                            cGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTg                                                                  <  2:117048/150‑1 (MQ=255)
                                                                             gCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGcc                                                                >  1:659917/1‑151 (MQ=255)
                                                                                 tCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCt                                                              <  1:267802/149‑1 (MQ=255)
                                                                                  cccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCttt                                                            <  1:312327/150‑1 (MQ=255)
                                                                                    cGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCg                                                         >  1:124032/1‑151 (MQ=255)
                                                                                      aTTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCt                                                       >  1:364280/1‑151 (MQ=255)
                                                                                      aTTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCt                                                       >  1:358561/1‑151 (MQ=255)
                                                                                        tGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTc                                                      >  1:804495/1‑150 (MQ=255)
                                                                                         ggCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTc                                                      >  1:846715/1‑149 (MQ=255)
                                                                                         ggCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCa                                                     <  2:346999/150‑1 (MQ=255)
                                                                                           cGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGt                                                   >  1:31948/1‑150 (MQ=255)
                                                                                               ttGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              >  2:38925/1‑151 (MQ=255)
                                                                                               ttGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              >  1:1000508/1‑151 (MQ=255)
                                                                                                tGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGACGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              <  2:313513/150‑1 (MQ=255)
                                                                                                 gCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgtcg                                            <  2:804449/151‑1 (MQ=255)
                                                                                                    gTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGATCAGTAAGgtcgtc                                          >  1:1020290/1‑150 (MQ=255)
                                                                                                      aaaaaaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCa                                       <  1:721180/151‑1 (MQ=255)
                                                                                                          aaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGt                                   >  1:781341/1‑151 (MQ=255)
                                                                                                          aaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGCCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGt                                   >  2:182055/1‑151 (MQ=255)
                                                                                                           aGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACCGCAATTTCAGTTTGCCCTTTTAGCTCAGTACGGTCGTCTCACCGTg                                  >  2:835423/1‑151 (MQ=255)
                                                                                                            gTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTgg                                 <  1:271712/151‑1 (MQ=255)
                                                                                                               cGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              <  1:556293/151‑1 (MQ=255)
                                                                                                                gAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              >  1:1040370/1‑150 (MQ=255)
                                                                                                                gAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              >  2:172238/1‑150 (MQ=255)
                                                                                                                  aTCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGGCTCACCGTGGCTg                              <  2:198846/148‑1 (MQ=255)
                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTTCTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCACTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaaa                         <  2:676832/151‑1 (MQ=255)
                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAGCGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaa                          <  2:911118/150‑1 (MQ=255)
                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaa                          >  1:947831/1‑150 (MQ=255)
                                                                                                                      ccACGGTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaaa                         >  2:374168/1‑149 (MQ=255)
                                                                                                                           gTCATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtg                  <  1:521997/151‑1 (MQ=255)
                                                                                                                             cATGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtgtg                <  1:171559/151‑1 (MQ=255)
                                                                                                                              aTGGCTTTCGCTGCCTCAGGGGGGCGTGCTTTTCGCCTCGCGATCCAGAACTCTTGCAACGTATTCTTCACGATCGAGATGCGACTGCAACTTCAGTTTGCCCTTTTCGCGCAGTAAGGTCGTCTCACCGTGGCTGACAAAc                        <  2:177324/142‑1 (MQ=255)
                                                                                                                              aTGGCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTgg               <  2:386213/151‑1 (MQ=255)
                                                                                                                                ggCTTTCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGtt             <  2:822249/151‑1 (MQ=255)
                                                                                                                                    ttCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTAcc          >  2:639468/1‑150 (MQ=255)
                                                                                                                                    ttCGCTGCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTAcc          <  1:420378/150‑1 (MQ=255)
                                                                                                                                          gCCTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCGCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa   <  2:555340/151‑1 (MQ=255)
                                                                                                                                           ccTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaa  <  2:234620/151‑1 (MQ=255)
                                                                                                                                           ccTCAGGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa   >  2:138494/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328635‑2328923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: