Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,737,713 (G)6→7 intergenic (+67/‑32) pheL → / → pheA pheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,737,7071.G91.9% 245.6 / 16.5 75intergenic (+61/‑38)pheL/pheApheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base G (34/34);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGG  >  NC_000913/2737564‑2737847
                                                                                                                                                |                                                                                                                                             
cGGCTACTATACTAAAGTCACTTAAGCAAACAAACATGAAACACATACCGGTTTTCTTAGCATACGTTTTTAACTGCCCCTGAATCGGAGTCAGTTCGTCGTGTGAGACAGAATGCGAAGACGAACAATAATGCCGCCCAGAACgggggg                                                                                                                                        <  2:183757/150‑1 (MQ=255)
     actgtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCcttt                                                                                                                                  >  2:759490/1‑151 (MQ=255)
        gtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCcttttt                                                                                                                                <  1:871491/150‑1 (MQ=255)
          actAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTa                                                                                                                               <  1:455782/150‑1 (MQ=255)
             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGTCGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTg                                                                                                                            >  1:724660/1‑150 (MQ=255)
             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTg                                                                                                                            >  2:1054334/1‑150 (MQ=255)
             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGa                                                                                                                           <  2:140177/151‑1 (MQ=255)
                  cACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACa                                                                                                                      <  1:313475/151‑1 (MQ=255)
                   aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACaa                                                                                                                     <  2:11751/151‑1 (MQ=255)
                    cTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACaa                                                                                                                     >  2:384372/1‑150 (MQ=255)
                       aaGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACaaaaa                                                                                                                  <  1:242263/150‑1 (MQ=255)
                         ggAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGc                                                                                                               <  2:41613/151‑1 (MQ=255)
                             acaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAac                                                                                                            >  2:366556/1‑150 (MQ=255)
                               aaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACt                                                                                                         >  1:45191/1‑151 (MQ=255)
                                aacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTa                                                                                                        <  1:275549/151‑1 (MQ=255)
                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTAt                                                                                                       >  1:590806/1‑151 (MQ=255)
                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTAt                                                                                                       <  2:1013710/151‑1 (MQ=255)
                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATg                                                                                                      <  1:803958/151‑1 (MQ=255)
                                     gAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGAc                                                                                                    <  1:289560/150‑1 (MQ=255)
                                     gAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGAc                                                                                                    <  1:55003/150‑1 (MQ=255)
                                         cacaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCgg                                                                                               <  1:1054389/151‑1 (MQ=255)
                                          acaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGa                                                                                              <  2:479269/151‑1 (MQ=255)
                                          acaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGa                                                                                              <  2:387672/151‑1 (MQ=255)
                                                cGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAAccc                                                                                        <  1:427457/151‑1 (MQ=255)
                                                     ttcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTa                                                                                    <  2:573742/150‑1 (MQ=255)
                                                      tcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACt                                                                                  >  1:543913/1‑151 (MQ=255)
                                                         tcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgc                                                                               >  2:899154/1‑151 (MQ=255)
                                                          cGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgcg                                                                              >  2:983422/1‑151 (MQ=255)
                                                            cATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgcgc                                                                             >  2:809064/1‑150 (MQ=255)
                                                             aTTCTTTTTTACCTTCCCGTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTg                                                                           <  1:42129/151‑1 (MQ=255)
                                                             aTTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTg                                                                           <  1:708355/151‑1 (MQ=255)
                                                              ttCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGc                                                                          >  2:807643/1‑151 (MQ=255)
                                                                cTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGc                                                                          >  2:798105/1‑149 (MQ=255)
                                                                cTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                         >  2:455999/1‑150 (MQ=255)
                                                                  tttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                         >  1:295315/1‑148 (MQ=255)
                                                                   ttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgag                                                                       >  1:268997/1‑149 (MQ=255)
                                                                   ttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgag                                                                       >  2:179305/1‑149 (MQ=255)
                                                                           tCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCa                                                              <  1:1060131/150‑1 (MQ=255)
                                                                             cccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcg                                                           >  2:309894/1‑151 (MQ=255)
                                                                              ccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgc                                                          <  1:288069/151‑1 (MQ=255)
                                                                               cTGATTGGGTGGCGTTTCGTCGTTTGATACAGAATTCGAAGGCGAACAATGAGGACTCCCAAATCGGTGGGGCCTTTTTTATTGATAACAAAAATCAAACACTATGACAGGTCAAAACCCGTTACTGACGCCGCGAGAGAAAATCAgcgc                                                          <  2:221514/150‑1 (MQ=255)
                                                                               cTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg                                                         >  2:333641/1‑151 (MQ=255)
                                                                                tGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcgc                                                        >  2:141639/1‑151 (MQ=255)
                                                                                 gAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCt                                                       <  1:84233/151‑1 (MQ=255)
                                                                                 gAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg                                                      >  1:945766/1‑151 (MQ=255)
                                                                                  aaTGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg                                                      >  1:673875/1‑151 (MQ=255)
                                                                                     gggAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGa                                                    <  1:992093/150‑1 (MQ=255)
                                                                                       gAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGa                                                 <  2:956847/151‑1 (MQ=255)
                                                                                        aGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaa                                                <  1:800092/151‑1 (MQ=255)
                                                                                            gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCTGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAat                                            >  1:1018866/1‑151 (MQ=255)
                                                                                            gTTGCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGAAACACTATGACAGCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaaaaa                                             <  2:706586/150‑1 (MQ=255)
                                                                                               tcgtcgTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCATTTTTATTGAGAAAAAAAAGGGAAAAATACGGCATTGGGAAAACCGGTACTGGCCACGCAAGGAAAAATCCACGGCGAGGATGAAAAattatt                                        >  2:121807/1‑151 (MQ=255)
                                                                                                 gtcgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAattatt                                        >  1:172509/1‑150 (MQ=255)
                                                                                                   cgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGc                                     <  1:376086/151‑1 (MQ=255)
                                                                                                   cgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGc                                     >  1:726292/1‑151 (MQ=255)
                                                                                                    gtggGAAACCGAAAGCGAAGACGAACAATAACCCATCCCAAATCGGGGGGGCCTATTTTATTGATAACACAAAGGCAACACAATGAAAGCGGAAAACCCGTTACTGGCCCTGCGATATAAAATCAGGGCGCCGGATGACAAAttatta                                       <  2:435256/148‑1 (MQ=255)
                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGtt                                  <  2:437746/151‑1 (MQ=255)
                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGtt                                  >  2:75057/1‑151 (MQ=255)
                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGt                                   <  1:886097/150‑1 (MQ=255)
                                                                                                        gAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTa                                 <  1:752077/150‑1 (MQ=255)
                                                                                                         aaaCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTAc                                >  2:213827/1‑150 (MQ=255)
                                                                                                          aaCAGAATGCGAAGACGAACAATACGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAACGGCAACACTATGACATAGGAAAACCCGGTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTg                              >  2:1042962/1‑151 (MQ=255)
                                                                                                           aCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTgg                             >  2:686363/1‑151 (MQ=255)
                                                                                                            cAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGc                            >  2:222128/1‑151 (MQ=255)
                                                                                                                    gAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGgc                    >  1:141865/1‑151 (MQ=255)
                                                                                                                          gAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAAc              >  2:619009/1‑151 (MQ=255)
                                                                                                                              aaTAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGc          >  2:465623/1‑151 (MQ=255)
                                                                                                                               aTAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGcc         <  2:742866/151‑1 (MQ=255)
                                                                                                                               aTAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGcc         >  2:905653/1‑151 (MQ=255)
                                                                                                                                   ggCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCg     <  1:626978/151‑1 (MQ=255)
                                                                                                                                      cTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGa                         >  2:258221/1‑128 (MQ=255)
                                                                                                                                      cTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGa                         <  1:258245/128‑1 (MQ=255)
                                                                                                                                      cTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  >  1:592507/1‑151 (MQ=255)
                                                                                                                                       tCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  >  2:317765/1‑150 (MQ=255)
                                                                                                                                        cccAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  <  2:614318/149‑1 (MQ=255)
                                                                                                                                        cccAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  <  1:958085/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGG  >  NC_000913/2737564‑2737847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: