Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,5220CA48.6% 37.5 / 17.3 37intergenic (‑540/+63)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (9/10);  new base A (18/0);  total (27/10)
Fisher's exact test for biased strand distribution p-value = 3.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTC  >  NC_000913/987373‑987671
                                                                                                                                                     |                                                                                                                                                      
aTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaacaaaca                                                                                                                                                       <  2:1353/151‑1 (MQ=255)
aTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaacaaaca                                                                                                                                                       <  2:774088/151‑1 (MQ=255)
    tATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGAt                                                                                                                                                    <  2:81683/150‑1 (MQ=255)
           aTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGCTTTACTTaaaaaacaaaaaa                                                                                                                                                  >  1:938185/1‑140 (MQ=255)
                gCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAATAATTCTTTAATTAAAAAAAAAAAAAAATGACAg                                                                                                                                         >  2:449562/1‑148 (MQ=255)
                    ttAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGCTTCCAATCAATTTCTTTTCTTAACAAAAAAATAAcctaccaccaaaaa                                                                                                                                    >  1:538265/1‑137 (MQ=255)
                      aGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATa                                                                                                                                 <  1:663368/150‑1 (MQ=255)
                          aaGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaaa                                                                                                                            <  1:646322/151‑1 (MQ=255)
                             aaaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAaga                                                                                                                         <  1:216512/151‑1 (MQ=255)
                             aaaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGCTTTATTTTAAAAAAAAAAAAAATTACAGaaaaaaaaaaaaaaa                                                                                                                          >  2:39238/1‑149 (MQ=255)
                              aaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTCGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGTTTGTCTTTACAAAAAAAAAAATTGACAGAAAAAATaaaaaaaaa                                                                                                                         >  2:729390/1‑148 (MQ=255)
                                    tAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTATTGAATTTAAAAACACAAAAATTTACaaaaaaaaaaaaaaaaaaagaca                                                                                                                    >  2:705496/1‑142 (MQ=255)
                                      aTCCGCAATAAGAATGCGGATTAGTTTTTGCACGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCggg                                                                                                                <  2:754552/151‑1 (MQ=255)
                                         cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTCTTGACTTTACAAAAAAAAAAATTGAACAAAAAAAAAAAAAAAAAAGCAAACgggg                                                                                                               >  1:49481/1‑149 (MQ=255)
                                               aaGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTGATTGTCTTATCAAAAAAATAACTTTACAGAAAAAAAAAAAAAAAAACAAACGGgcggcgggt                                                                                                        >  2:475099/1‑144 (MQ=255)
                                               aaGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGGGGAAAAAAAATGCTTTAGTTTACAAAAAGATAAAATTAAAGAAAAAAAAAAAAAAAAGACAGCGGGGGCCGGctc                                                                                                       >  2:317584/1‑151 (MQ=255)
                                                aGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGCTTGACTTTACAAAAAAATAAATTTACAGAAAAAATAAAAAAAAAGGCAGCGGGCGCTgg                                                                                                          >  1:311055/1‑147 (MQ=255)
                                                   aTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGATTGCTTTACTTTACAAACAAATAACTTTACAGAAAAAAAAAAAAAAAAAACAGCGGGCGCTGGCTCtt                                                                                                     >  1:463257/1‑149 (MQ=255)
                                                    tGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTTTTTTCTTAACAAAAAAAAAAAATGACAAAAAAGAAAAAAAAAAAAACAACGGGGGCTGGCGCtt                                                                                                     >  1:658163/1‑148 (MQ=255)
                                                     gtggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGCAATAAAATAGTTGTATTTAAAAAAAAATAAATTGACAGAAAAAAAAAAAAAAAAGACAGCGGGCGCCgggcctt                                                                                                     >  2:237583/3‑142 (MQ=255)
                                                       ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCTAAAAGATTGTTTGTCTTTAAAAACAGATAACATGACAGAAAAAATAAAAAAAAAAACAGAGGGGGCGGGCCCTTGaa                                                                                                  >  1:107384/1‑148 (MQ=255)
                                                           tAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCt                                                                                            <  2:642723/150‑1 (MQ=255)
                                                               ttttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTCTTGACTTTAAAAAAAAAAAAATTTTCAGAAAAAAAAAAAAAAAAGACAGCGGGGGCCGGGGCTTTAAGAAGCCaaa                                                                                         >  1:608359/1‑149 (MQ=255)
                                                                tttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGCAAAAAATTGGTTGACTTAACAAAAAAAAAAATTTAAAGAAAAAAAAAAAAAAAAACCCACGGGGGCCGGCTCCTTGAGAAGCTaaa                                                                                         >  1:1005129/1‑148 (MQ=255)
                                                                     gcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTCTTTACTTAAAAAAAAAATAAATTTACAGAAAAAAAAAAAAAAAAAACAACGGGGGCTGGGCCTTTAAGAAGCCAAAATAGaa                                                                                   >  1:336272/1‑149 (MQ=255)
                                                                       gTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTg                                                                               <  1:562853/151‑1 (MQ=255)
                                                                        tAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGGGCTAAAAAAATGTTTTAGTTTAAAAAAAAATAAACTGACAGAAAAAATAAAAAAAAAAAAAGCGGGGGGCGGGGCTTGTAGAAGCCAAAATAGaaaa                                                                                 >  1:860748/1‑146 (MQ=255)
                                                                         aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGc                                                                             <  1:1051389/151‑1 (MQ=255)
                                                                         aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGGAAAAAATTGCTTGTCTTTAAAAAAAAATAACTTTACAGAAAAAAAAAAAAAAAAGACAGCGGGGGCCGGCTCTTTAAGACGCCAAAATaaaaaacg                                                                               >  1:355322/1‑145 (MQ=255)
                                                                         aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGCAATAAATTGGTTGCCTTAAAAAACAAAAAAATTTACAGAAAAGAAAAAAAAAAAACCAACGGGGGCGGGGTCTTGTAGAAGCCAAATTaaa                                                                                    >  1:840648/1‑142 (MQ=255)
                                                                            tGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTGGGAAAAAGAATTGTTTTCTTAACAAAAAAATAAAATGTACGAAAAAAAAAAAAAAAAAACAACGGGCGCTGGCCCTTTGAGAAGCCAAAATaaaaaatgggggt                                                                          >  2:1043937/1‑142 (MQ=255)
                                                                                  aaTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAATTTCTTTTTTTAAAAAACAGATAAATTGACAGAAAAAAAAAAAAAAAAGACAGCGGGGGCTGGGGCTTTAAGACGACAAAATAGAAAAGGGCGTTACGCgg                                                                    >  2:589422/1‑151 (MQ=255)
                                                                                       aaTATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTAc                                                               <  1:928136/151‑1 (MQ=255)
                                                                                       aaTATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAGATTGTTTTTATTTAAAAACAAAAAAATTTAAAGAAAAAATAAAAAAAAAAACAACGGGGGCCGGCCCTTTTAGAAGCCAAAATAAAAAATGGCGGTTCGCgggg                                                                  >  1:1009989/1‑146 (MQ=255)
                                                                                                tAACAGGATGATCGTTATGGCGGGGGTGGCAATAGAATGGTTGGCTTTAAAAAAAGAAAAAATGAACAAAAAGAAAAAAAAAAAGGCAGCGGGGGGGGGCTCCTGAAAAAGCCAAAATATAAGATGGCGGTAAGCCGAAAACGTGGGaa                                                        >  1:625122/1‑149 (MQ=255)
                                                                                                          atCGTTATGGCGGGGTTGCAAAAAAATTGCTTGACTTAAAAAAAAGATAAAATGACAGAAAAAATAAAAAAAAAACCAGCGGGCGCTGGCTCTTTTAAACGCCAAAATAAAAACTGGCGTTACGCGGAGGACGTGGGGAAGGGatca                                                >  2:132539/1‑147 (MQ=255)
                                                                                                                  ggCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtacgt                                    <  1:112510/151‑1 (MQ=255)
                                                                                                                          tGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACg                            >  1:864589/1‑151 (MQ=255)
                                                                                                                                           aCTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAAt           >  1:708764/1‑151 (MQ=255)
                                                                                                                                              tAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATc          >  2:538374/1‑149 (MQ=255)
                                                                                                                                               aacaaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATc          >  1:499792/1‑148 (MQ=255)
                                                                                                                                                acaaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTATGTTTTGCACGCCAGTTACGTAAGCAATCAGAc      <  2:574533/151‑1 (MQ=255)
                                                                                                                                                 caaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACg     <  1:558506/151‑1 (MQ=255)
                                                                                                                                                   aacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt   <  2:572645/151‑1 (MQ=255)
                                                                                                                                                   aacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt   >  2:122756/1‑151 (MQ=255)
                                                                                                                                                     caGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTc  >  2:959186/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTC  >  NC_000913/987373‑987671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: