Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC42.4% 78.0 / 22.9 59V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (28/6);  new base C (0/25);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 4.54e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAT  >  NC_000913/993770‑994063
                                                                                                                                                     |                                                                                                                                                  
tgCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacc                                                                                                                                                  >  2:316791/2‑150 (MQ=255)
 gCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCcaccac                                                                                                                                                >  1:753198/1‑151 (MQ=255)
    gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCATGGTACTTTTGCCACCTCCGCTGCGGCCCACCACCg                                                                                                                                              <  2:10099/150‑1 (MQ=255)
         gcATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCcaca                                                                                                                                         >  2:876078/1‑150 (MQ=255)
           aTCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAAc                                                                                                                                      >  1:704454/1‑151 (MQ=255)
           aTCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAAc                                                                                                                                      >  2:275712/1‑151 (MQ=255)
                  aaCATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTACAGACCTGCCAGCAGGCGCAGAAGGGTAATTTTGCAACCACCGCTGCGGCCAACAACCGCCACAAACTGAc                                                                                                                                  >  2:739643/1‑148 (MQ=255)
                     atcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCg                                                                                                                            >  2:179102/1‑151 (MQ=255)
                           ccgttttcctCCTGATTTTCAGCCAGGGGGGTGGCCCCCCTTAACACACCCCCGGCGTTTGGCTTTTCAGGACCGCCCAGCGGGCGCAACGGGGTCTTTTTCCCACCCCCCCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAata                                                                                                                       <  1:276038/141‑1 (MQ=255)
                             cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                    >  1:78212/1‑151 (MQ=255)
                             cgTGTCTCCCTAATTTTCACCCGGCGGGGGGGGGCCCGCTACCCCACCGCCTGGGGTTGGCGTTCCCAGCCCTCCCACCTGCCGCAGCGGGTTACTTTTCCCACCTCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAat                                                                                                                        <  1:817954/147‑1 (MQ=255)
                              ttgtCTCCCAGAATTCCAGCCGGGGGTGGGGCGCCCGCTAACCCATCGCGGGCGTTTGGTGTTCCCACCCCGGCCGGCGGGCCCAGCAGGTTTCTTGTGCCACCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGaa                                                                                                                         <  2:17387/144‑1 (MQ=255)
                              gtgtCTTCGTAAATTCCCGCCGGGGGTGGGGTGCCCCCTAACACCCCGCCTGCGTTTGGCGTCCCCGCCCCCCCCAGCGGGCGCGGCGGGTTTTTTTTGCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAata                                                                                                                       <  2:1052486/147‑1 (MQ=255)
                                 tCTTCCTGAATTTCAGCCGGGGGTGGGGTGCCCGCAAACACACCGCCGGCGGTTGGCTTTCCCAGCCCTGCCAGCAGGCGCAGCGGGGTCCTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTaaa                                                                                                                 <  1:943868/150‑1 (MQ=255)
                                 tCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAAt                                                                                                                >  1:200356/1‑151 (MQ=255)
                                     cgtaattttCAGCCAGGGGTGTGGTCCCCGCTAACACAGCGCCGGCGGTGGGCGTTTCCAGCCCTGCCGGGGGGCGCAGCAGGGTTCTTTTGCCACCACCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAg                                                                                                            <  1:416472/145‑1 (MQ=255)
                                     ccTGAATTTCAGCCGGGGGTGGGGGCCCCGCTAACACATCGCCGGGGGTGGCCTTTCCCAGCCCTCCCAGCGGGCGCAGAGGGGTCTTTTCCCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                     <  2:98866/142‑1 (MQ=255)
                                      cTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGt                                                                                                           >  1:397598/1‑151 (MQ=255)
                                      cTGAATTCCAGCCGGGGGTGGGGGGCCCGCTAACACACCGCCTGCGGTTGGCTTTCCCAGACCTGCCAGCAGGCGCAGCGGGTTCTTTTTCCCACCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATc                                                                                                               <  1:674806/147‑1 (MQ=255)
                                       tGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                                          >  2:439977/1‑151 (MQ=255)
                                        gatttccacccaggGGTGTGGTCCCCGAAAACCCAGCCCTGGGGGTGGGCGTGCCCAGCCCCGCCAGCGGGGGCAGCAGGTTACTTTTGCCCCCCCCGGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGt                                                                                                           <  2:367883/140‑1 (MQ=255)
                                          aTTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                       >  1:548927/1‑151 (MQ=255)
                                           ttccagccgggtgtgtGGTCCCCGCAAACACACCCCCGGCGGTGGGCGTTCCCACCCCTGCCAGCAGGCGCAGAGGGTTCTTTTTCCCACCACCCCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                       <  2:99989/138‑1 (MQ=255)
                                             tCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                     >  2:420328/1‑150 (MQ=255)
                                             tCAGCCAGCGGTGTGGTGCCCGCTAACACATCGACTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                     >  1:1026751/1‑150 (MQ=255)
                                              cagccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                     >  2:1035024/1‑149 (MQ=255)
                                                      ggtggggcccccGCAAACCCACCGCCTGGGGTTGCTTTTCCCAGCCCGCCCGCCAGGCGCAGCGGGTTCTTTTTCCCCCCCCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat                                                                                           <  2:13163/142‑1 (MQ=255)
                                                                ccGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGt                                                                                  >  1:696566/1‑150 (MQ=255)
                                                                     aaCACATCCCCTGTGTTTGGCGTTTCCACCCCTGCCAGCGGCGGCAGCAGGTTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                                <  1:631865/147‑1 (MQ=255)
                                                                         caccGCCGGCGGTTGGCGTTCCCAGCCCTGCCAGCAGGCGCAGCGGGGTATTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                        <  1:217291/148‑1 (MQ=255)
                                                                          accGCCTGGGGTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCAGGGTTCTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGc                                                                         <  1:220146/147‑1 (MQ=255)
                                                                              ctggCGGTGGGCTTTCCCAGCCCTGCCAGCAGGCGCAGCGGGTTTTTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                                      <  1:911838/145‑1 (MQ=255)
                                                                              ccTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTg                                                                   >  2:174631/1‑151 (MQ=255)
                                                                               cTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTg                                                                   >  1:405061/1‑150 (MQ=255)
                                                                                  tggTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAtt                                                               >  2:946998/2‑151 (MQ=255)
                                                                                  gggtgggtgTCTCCAGCCCTGACGGCAGGCCAGGCAGGGTATTTTGCCCCCCTCCGTTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACt                                                                    <  1:773082/145‑1 (MQ=255)
                                                                                     tgggCTTTCCCAGCCCTGCCAGCAGGCGCAGCGGGGTTTTTTGGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGa                                                             <  1:491351/148‑1 (MQ=255)
                                                                                            ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                     >  1:121726/1‑151 (MQ=255)
                                                                                            ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                     >  1:326464/1‑151 (MQ=255)
                                                                                              cccgccccgCCGGCAGGCGCAGCAGGTTCCTTTTCCCCCCCCCGCTCGGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                           <  2:1038062/135‑1 (MQ=255)
                                                                                              ccacccccgCCAGCGGCCGCAGCAGGTTATTTTTCCCCCCGCCGCGGCCCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg                                                   <  2:339101/143‑1 (MQ=255)
                                                                                                agcccccccAGCAGGCCCAGCAGGGTCCTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg                                                    <  1:901463/141‑1 (MQ=255)
                                                                                                  cccgcccAGCAGGCGCAGCGGGTTTTTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                               <  1:844784/146‑1 (MQ=255)
                                                                                                   ccTCCCAGCGGGCGCAGCGGGGTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                                 <  1:782526/148‑1 (MQ=255)
                                                                                                       ccAGCGGGCGCGGCAGGCTTGTTTCCCCCCCACCGTCGCCCCCCCCACCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTg                                          <  1:929402/151‑1 (MQ=255)
                                                                                                       ccAGCAGGCGCAGCAGGGTATTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCt                                           <  1:156715/150‑1 (MQ=255)
                                                                                                         agcagGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGt                                        >  1:870522/1‑151 (MQ=255)
                                                                                                          gcagGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGtt                                       >  1:219816/1‑151 (MQ=255)
                                                                                                               cgcAGCGGGTTTCTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGAc                                  <  1:731558/151‑1 (MQ=255)
                                                                                                                 cagcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                 >  2:237321/1‑150 (MQ=255)
                                                                                                                  agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGaag                                 >  2:448643/1‑147 (MQ=255)
                                                                                                                    cagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                              >  2:403651/1‑150 (MQ=255)
                                                                                                                      gggTTCTTTTCCCCCCCCCTCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                           <  1:632419/151‑1 (MQ=255)
                                                                                                                       ggttttttttCCCCCCCCCGCTGCCCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                                <  2:871351/140‑1 (MQ=255)
                                                                                                                       ggttatttttCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa                                   <  1:213293/137‑1 (MQ=255)
                                                                                                                       ggTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAATTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                           >  2:451196/1‑150 (MQ=255)
                                                                                                                        gTACTTTTTCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGATCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAt                         >  2:257543/1‑151 (MQ=255)
                                                                                                                             ttttCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCa                      <  1:573892/150‑1 (MQ=255)
                                                                                                                             ttttCCCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc                       <  1:340937/149‑1 (MQ=255)
                                                                                                                              tttGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                     >  2:830061/1‑150 (MQ=255)
                                                                                                                                           gCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcct        >  2:87251/1‑151 (MQ=255)
                                                                                                                                                 cccccccccCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt  <  2:252508/150‑1 (MQ=255)
                                                                                                                                                 cccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCtt       <  1:102514/145‑1 (MQ=255)
                                                                                                                                                 cccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt  <  2:576774/150‑1 (MQ=255)
                                                                                                                                                  ccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa     <  1:316815/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAT  >  NC_000913/993770‑994063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: