Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,257	T→C	Y552C (TAC→TGC)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,257	0	T	C	100.0%	219.0 / NA	59	Y552C (TAC→TGC)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/25);  total (34/25)

AGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGG  >  NC_000913/3084116‑3084397                                                                                                                                              |                                                                                                                                             aGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggc                                                                                                                                      >  2:14481/1‑150 (MQ=255)    tctacttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcatt                                                                                                                                   <  1:133540/150‑1 (MQ=255)       acttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattggca                                                                                                                               <  1:157703/151‑1 (MQ=255)        cttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattgg                                                                                                                                 <  2:36153/148‑1 (MQ=255)              cGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGt                                                                                                                        >  1:1057205/1‑151 (MQ=255)               gCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTg                                                                                                                       <  1:887904/151‑1 (MQ=255)                 tACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGc                                                                                                                     >  1:22965/1‑151 (MQ=255)                  aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTgg                                                                                                                      <  1:353133/149‑1 (MQ=255)                         gggAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGt                                                                                                             >  2:714035/1‑151 (MQ=255)                             aGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCAcc                                                                                                         <  1:675479/151‑1 (MQ=255)                               aCGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCAt                                                                                                       >  2:15885/1‑151 (MQ=255)                                cGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATc                                                                                                      >  1:62575/1‑151 (MQ=255)                                    cacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAat                                                                                                   >  1:809887/1‑150 (MQ=255)                                        tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAg                                                                                               <  2:609792/150‑1 (MQ=255)                                          aaCCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTgg                                                                                            >  2:121764/1‑151 (MQ=255)                                           aCCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGt                                                                                           <  1:121766/151‑1 (MQ=255)                                                      aTCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCAcc                                                                                 <  1:1024277/150‑1 (MQ=255)                                                        cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGt                                                                               <  1:992500/150‑1 (MQ=255)                                                         aCCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTc                                                                              >  1:200837/1‑150 (MQ=255)                                                             aaCAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGCAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCagag                                                                          >  1:158193/1‑150 (MQ=255)                                                              aCAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTc                                                                        >  1:172591/1‑151 (MQ=255)                                                                       gCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAat                                                               <  1:47198/151‑1 (MQ=255)                                                                        cATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAata                                                              <  2:915693/151‑1 (MQ=255)                                                                         aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                             >  1:53499/1‑151 (MQ=255)                                                                         aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                             <  1:14481/151‑1 (MQ=255)                                                                         aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                             >  2:502989/1‑151 (MQ=255)                                                                             tGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATcc                                                           >  2:751907/1‑149 (MQ=255)                                                                               ccGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagca                                                       <  2:834439/151‑1 (MQ=255)                                                                               ccGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagca                                                       <  1:984969/151‑1 (MQ=255)                                                                                cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagcag                                                      <  1:42936/151‑1 (MQ=255)                                                                                cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagc                                                        >  1:1013927/1‑149 (MQ=255)                                                                                 gAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCagcagc                                                     <  1:676853/151‑1 (MQ=255)                                                                                     aTCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGcac                                                   >  1:758167/1‑149 (MQ=255)                                                                                      tctcCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAgc                                                >  1:976074/1‑151 (MQ=255)                                                                                         ccTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAgcgc                                              <  1:48036/150‑1 (MQ=255)                                                                                            gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACg                                          >  2:481475/1‑151 (MQ=255)                                                                                              tatGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGtt                                        >  1:100599/1‑151 (MQ=255)                                                                                               atGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGtt                                        <  2:62574/150‑1 (MQ=255)                                                                                               atGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTc                                       >  1:149844/1‑151 (MQ=255)                                                                                                 gcgcCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCg                                     >  2:263822/1‑151 (MQ=255)                                                                                                 gcgcCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCg                                     <  2:100597/151‑1 (MQ=255)                                                                                                  cgcCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCgg                                    <  2:872144/151‑1 (MQ=255)                                                                                                  caccGACCATAAAGAAAGCGAGCATCGGCGGAATCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGGGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGAc                                           >  2:474895/3‑144 (MQ=255)                                                                                                       aCCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACtt                               >  1:390914/1‑151 (MQ=255)                                                                                                             aaGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCa                         <  1:425405/151‑1 (MQ=255)                                                                                                              aGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATcc                          >  1:670293/1‑149 (MQ=255)                                                                                                              aGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATcc                          >  1:907774/1‑149 (MQ=255)                                                                                                                aaaCCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGcc                      >  1:585838/1‑151 (MQ=255)                                                                                                                   ccGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCtt                   <  1:554859/151‑1 (MQ=255)                                                                                                                   ccGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCtt                   >  1:233177/1‑151 (MQ=255)                                                                                                                      aGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTcc                 >  2:823878/1‑150 (MQ=255)                                                                                                                          tCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCgg            >  2:887894/1‑151 (MQ=255)                                                                                                                            ggcggATTCTCGGGATCGCACTCCGGCATTGGCATTGTCGTGGCAAAACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc           >  2:99521/1‑150 (MQ=255)                                                                                                                             gcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc           >  1:482569/1‑149 (MQ=255)                                                                                                                                 aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc     <  1:62498/151‑1 (MQ=255)                                                                                                                                   tctcTGGATCGCACTCCGGCAGTGGCATTGTCGTGGCAATACCGACCGCATCAGTTTAGTGGTCGATAGCACGGTCAGAGTCACTGGTAATATCCAGCAGCACAGCGCGACGATCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCgg   >  2:543498/1‑151 (MQ=255)                                                                                                                                     tctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg  >  1:426923/1‑150 (MQ=255)                                                                                                                                        ggATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGcc                      >  2:739112/1‑127 (MQ=255)                                                                                                                                        ggATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGcc                      <  1:739153/127‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             AGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGG  >  NC_000913/3084116‑3084397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑