Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,695	T→C	Q294Q (CAA→CAG)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,695	0	T	C	100.0%	240.1 / NA	65	Q294Q (CAA→CAG)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

TCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGT  >  NC_000913/3372556‑3372842                                                                                                                                            |                                                                                                                                                    tCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAAct                                                                                                                                           <  2:254722/150‑1 (MQ=255)    gggTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAActgctg                                                                                                                                       >  2:97217/1‑151 (MQ=255)           agtaCTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAgc                                                                                                                                >  1:183879/1‑151 (MQ=255)           agtaCTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAg                                                                                                                                 <  2:188215/150‑1 (MQ=255)               cTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCt                                                                                                                            >  1:167693/1‑151 (MQ=255)                tCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCtt                                                                                                                           >  1:155484/1‑151 (MQ=255)                 cATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGTGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCtt                                                                                                                           <  2:875801/150‑1 (MQ=255)                  aTTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTc                                                                                                                          >  1:250582/1‑150 (MQ=255)                  aTTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCa                                                                                                                         <  2:9465/151‑1 (MQ=255)                         gTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTgg                                                                                                                  <  1:231137/151‑1 (MQ=255)                             cccGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGc                                                                                                                 <  2:470819/148‑1 (MQ=255)                                gcgGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGatat                                                                                                           >  2:1000293/1‑151 (MQ=255)                                  ggTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATAttt                                                                                                         <  2:770688/151‑1 (MQ=255)                                   gTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATAtttt                                                                                                        <  1:419103/151‑1 (MQ=255)                                   gTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATAtttt                                                                                                        >  2:395066/1‑151 (MQ=255)                                   gTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATAtttt                                                                                                        >  1:808277/1‑151 (MQ=255)                                     gggCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTc                                                                                                      <  1:593338/151‑1 (MQ=255)                                         gATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCt                                                                                                  <  1:236846/151‑1 (MQ=255)                                          aTGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTa                                                                                                 <  1:374817/151‑1 (MQ=255)                                             cccACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCg                                                                                              <  1:886461/151‑1 (MQ=255)                                              ccACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCgg                                                                                             >  2:619986/1‑151 (MQ=255)                                                acacGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTc                                                                                           <  1:36332/151‑1 (MQ=255)                                                 cacGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTcc                                                                                          >  1:994220/1‑151 (MQ=255)                                                    gCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCaaa                                                                                       <  1:254681/151‑1 (MQ=255)                                                      tttGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACg                                                                                     >  2:836617/1‑151 (MQ=255)                                                      tttGGAATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACg                                                                                     >  2:524181/1‑151 (MQ=255)                                                       ttGGTATGAAAATTGTAGGGAACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCAACCGCGCTCCTGCATCAACTGCTGGACCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACgg                                                                                    >  2:703340/1‑151 (MQ=255)                                                            aTGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTg                                                                                <  1:433974/150‑1 (MQ=255)                                                               aaaaTTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggc                                                                            >  2:123413/1‑151 (MQ=255)                                                               aaaaTTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggc                                                                            >  1:1000422/1‑151 (MQ=255)                                                                  aTTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggcaca                                                                         <  1:490779/151‑1 (MQ=255)                                                                     gTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggcacagcg                                                                      >  1:110629/1‑151 (MQ=255)                                                                       aGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggcacagcggc                                                                    <  1:147203/151‑1 (MQ=255)                                                                       aGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggcacagcgg                                                                     <  2:172470/150‑1 (MQ=255)                                                                       aGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTgcggcacagcgg                                                                     <  2:458237/150‑1 (MQ=255)                                                                            aCAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGa                                                               <  1:261353/151‑1 (MQ=255)                                                                             cAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGa                                                               >  2:227344/1‑150 (MQ=255)                                                                             cAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGa                                                               >  2:45964/1‑150 (MQ=255)                                                                                aTGCGTTTATTTCCCCTCACCCTGTAGGGGCGAGCGCGGGGAAACAACTCACTCGCTCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGcaa                                                             >  2:61000/1‑149 (MQ=255)                                                                                      ttATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATc                                                      <  1:45965/150‑1 (MQ=255)                                                                                       tATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATcc                                                     <  2:599231/150‑1 (MQ=255)                                                                                        aTTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCat                                                   <  1:172196/151‑1 (MQ=255)                                                                                         tttCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTc                                                                                           <  2:787745/110‑1 (MQ=255)                                                                                         tttCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTc                                                                                           >  1:787787/1‑110 (MQ=255)                                                                                         tttCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCat                                                   >  1:179255/1‑150 (MQ=255)                                                                                         tttCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCat                                                   >  2:826794/1‑150 (MQ=255)                                                                                          ttCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCatat                                                 >  1:507841/1‑151 (MQ=255)                                                                                             cccTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCAtata                                                <  2:313671/149‑1 (MQ=255)                                                                                                tCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATgg                                            >  1:371456/1‑150 (MQ=255)                                                                                                  aCCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATggagg                                         >  1:171826/1‑151 (MQ=255)                                                                                                    ccGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGAc                                       >  2:197193/1‑151 (MQ=255)                                                                                                       gTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGt                                    <  1:308487/151‑1 (MQ=255)                                                                                                         aGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGttt                                  <  1:618384/151‑1 (MQ=255)                                                                                                             gcgagcgaGGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTc                                >  1:1060280/1‑149 (MQ=255)                                                                                                                agcgagGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGcc                           <  1:1004468/151‑1 (MQ=255)                                                                                                                agcgagGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGcc                           <  1:44082/151‑1 (MQ=255)                                                                                                                 gcgagGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCcg                          <  2:963731/151‑1 (MQ=255)                                                                                                                    agGGGAAACAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCgg                       <  1:315833/151‑1 (MQ=255)                                                                                                                         aaaCAACTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATAc                  <  1:596338/151‑1 (MQ=255)                                                                                                                            caacTCACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGcc               <  1:97219/151‑1 (MQ=255)                                                                                                                                 cACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGttt          <  1:867247/151‑1 (MQ=255)                                                                                                                                 cACCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGtt           <  2:534170/150‑1 (MQ=255)                                                                                                                                  aCCCGCGCTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGtttt         <  1:467619/151‑1 (MQ=255)                                                                                                                                      gcgcTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATc     <  1:237602/151‑1 (MQ=255)                                                                                                                                       cgcTCCTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCa    <  2:641653/151‑1 (MQ=255)                                                                                                                                         ctccTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGt  >  1:706973/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                    TCTGGGTTGTAGTACTCATTGTGAGTCTCCCGCGGTGGGCGATGCCCACACGCTTTGGTATGAAAATTGTAGGGTACAGATGCGTTTATTTCCCCTCACCCGGTAGGGGCGAGCGAGGGGAAACAACTCACCCGCGCTCTTGCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGT  >  NC_000913/3372556‑3372842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑