Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,585,493 T→C Y444C (TAC→TGC)  ggt ← gamma‑glutamyltranspeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,585,4930TC98.2% 186.6 / NA 55Y444C (TAC→TGC) ggtgamma‑glutamyltranspeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (22/31);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACC  >  NC_000913/3585347‑3585636
                                                                                                                                                  |                                                                                                                                               
gcagcaCTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAAc                                                                                                                                             >  2:161715/1‑151 (MQ=255)
   gcaCTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGtt                                                                                                                                          <  2:719186/151‑1 (MQ=255)
        gTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGt                                                                                                                                      <  2:707821/150‑1 (MQ=255)
            tgatgaTCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGcc                                                                                                                                 >  1:316883/1‑151 (MQ=255)
             gatgatCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGccc                                                                                                                                <  1:498936/151‑1 (MQ=255)
              atgctCCTGCTACCGCATGGGCTACCGGTAACCAGCCAGGTTTTACCGCCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACAAGCCCGCAAACGTTCGGTACGCCCg                                                                                                                               >  2:1009337/1‑151 (MQ=255)
              atgatCCGGCTACCCCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCTGTACGcca                                                                                                                                <  2:621659/150‑2 (MQ=255)
                gatCCGGCTACCGCCTGGGCTACCGGTCACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGAGTTGGCATCACAGCCCACCAGCCCGCAAACGTTCGGTACGCCCgg                                                                                                                              >  2:849904/1‑150 (MQ=255)
                   ccGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGttt                                                                                                                           <  2:1037639/150‑1 (MQ=255)
                      gCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCg                                                                                                                       <  2:324090/151‑1 (MQ=255)
                          ccGCGTGGGCTTCCGGTAACCAGCCAGGTTTTACCTTCTTTCACCACAATGGTCGGCGACATCGTCGACTTCGGGCGTTTCTTCGGCCGGCCGGCGTTGGCATCACCGCCCACCAGCCCCCAAATGTTCGGTACGACCGGTTTGGCGgag                                                                                                                    <  2:741979/150‑1 (MQ=255)
                                gggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAatca                                                                                                              <  2:1028302/150‑1 (MQ=255)
                                      ccGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCt                                                                                                       <  2:634719/151‑1 (MQ=255)
                                        ggTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTgg                                                                                                     <  1:22057/151‑1 (MQ=255)
                                            aCCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAACTCATCCATCTGGttat                                                                                                 <  1:214149/151‑1 (MQ=255)
                                                  cagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCa                                                                                           <  1:829189/151‑1 (MQ=255)
                                                    ggTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGc                                                                                            >  1:610744/1‑148 (MQ=255)
                                                    ggTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGc                                                                                            >  1:627943/1‑148 (MQ=255)
                                                    ggTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGa                                                                                         <  1:159402/151‑1 (MQ=255)
                                                         tACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATAcc                                                                                    >  1:681453/1‑151 (MQ=255)
                                                         tACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATAc                                                                                     <  2:884288/150‑1 (MQ=255)
                                                          aCCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCg                                                                                   <  2:1058353/151‑1 (MQ=255)
                                                             gTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCCGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCCTCCATCTGGTTATTAAGCAGAATACCGctc                                                                                >  2:837729/1‑151 (MQ=255)
                                                                      cacaATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCcgcg                                                                       >  1:274054/1‑151 (MQ=255)
                                                                        caATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGAc                                                                     >  2:415325/1‑151 (MQ=255)
                                                                                ggagACATCGGAGACAGCGGGCGTGGGTGCGGCCCGAAGGCGCTGGTATCACCGAACACCAGCCAGTATACGTACGGGTCGCCCGGTTTGGCGGAGACATCATCCATCTGGTTATTAAGAAGAAGACCGCTCTCGCACGAGACaaggac                                                               <  2:234265/146‑5 (MQ=255)
                                                                                    aCATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGCAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTAcc                                                         >  2:584024/1‑151 (MQ=255)
                                                                                      aTCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGa                                                       <  1:861225/151‑1 (MQ=255)
                                                                                           cgacAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAggtg                                                  >  2:310799/1‑151 (MQ=255)
                                                                                             acAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAggtggt                                                <  1:519769/151‑1 (MQ=255)
                                                                                              cAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGgtggtg                                               <  1:832325/151‑1 (MQ=255)
                                                                                              cAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGgtggtg                                               <  1:686399/151‑1 (MQ=255)
                                                                                               aGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACGAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGgtgt                                              <  2:252282/151‑1 (MQ=255)
                                                                                               aGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGgtgt                                              >  1:114848/1‑151 (MQ=255)
                                                                                                gCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                             >  2:489006/1‑151 (MQ=255)
                                                                                                 cGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTc                                            >  1:192356/1‑151 (MQ=255)
                                                                                                  gggCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTc                                            >  1:4111/1‑150 (MQ=255)
                                                                                                  gggCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCa                                           <  1:174073/151‑1 (MQ=255)
                                                                                                     cGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGGGGTGTTCAGCg                                        >  1:963723/1‑151 (MQ=255)
                                                                                                        ttGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGta                                      <  2:536280/150‑1 (MQ=255)
                                                                                                          gTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAg                                   <  2:540989/151‑1 (MQ=255)
                                                                                                               gCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcac                              <  1:163022/151‑1 (MQ=255)
                                                                                                                  cGACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgc                           <  1:105818/151‑1 (MQ=255)
                                                                                                                   gACGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgcc                          <  2:514136/151‑1 (MQ=255)
                                                                                                                    aCGGCGTTGGCATCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgcc                          >  2:33217/1‑150 (MQ=255)
                                                                                                                     cGGCGTTGTCATCACCGCCCACCAGCCCGAAAACATTCAGTAGGAACGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAACAAAAGCCCGCGCTCGAACACGACAATGCCAGTACCAATAGTTGTGGTCAGCGTATAGGAcaccgcca                         <  2:813777/150‑1 (MQ=255)
                                                                                                                      ggCGTTGGCTCCCCCGCCCCCCAGCCCCCAAACGTGCGGTACGCCCGTTTTGGCGTAGAAACCATCTTTCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGAAATTGCCCGTCCGGAGGGTGGTGTTCGGCTTATAGGTcaccgccac                        <  2:816603/150‑1 (MQ=255)
                                                                                                                                tCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                             <  2:994666/119‑1 (MQ=255)
                                                                                                                                tCACCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                             >  1:994718/1‑119 (MQ=255)
                                                                                                                                  aCCGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCt           <  1:458942/151‑1 (MQ=255)
                                                                                                                                   ccGCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCtt          <  1:737603/151‑1 (MQ=255)
                                                                                                                                     gCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCttt         >  1:286511/1‑150 (MQ=255)
                                                                                                                                     gCCCACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCttt         >  1:994350/1‑150 (MQ=255)
                                                                                                                                      cccACCAGCCCGCAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTAt       <  1:191995/151‑1 (MQ=255)
                                                                                                                                            aGCCCGCAAACGCTCGGTACGCCCGGCTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATccacc  >  2:809225/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACC  >  NC_000913/3585347‑3585636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑