Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,755,937	A→G	V192A (GTG→GCG)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,755,937	0	A	G	100.0%	180.8 / NA	53	V192A (GTG→GCG)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

TTTGGCGATGCGTTTCGAGGTCGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCACCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATC  >  NC_000913/3755789‑3756080                                                                                                                                                     |                                                                                                                                                tttGGCGATGCGTTTCGAGGTCGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGcgcc                                                                                                                                               >  1:1004240/1‑151 (MQ=255)  ttGGCGATGCGTTTCGAGGTCGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGcgcc                                                                                                                                               <  2:307747/150‑1 (MQ=255)   tGGCGATGCGTTTCGAGGTCGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGcgcca                                                                                                                                              <  1:955331/150‑1 (MQ=255)                   ggTCGCCAGATATTCGCCAATTCCCCCCCCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCCCCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCcgccgc                                                                                                                              <  2:709290/150‑1 (MQ=255)                      cGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAg                                                                                                                           <  1:256717/150‑1 (MQ=255)                              atTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGc                                                                                                                  >  2:271166/1‑151 (MQ=255)                               ttCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGcc                                                                                                                 >  1:807273/1‑151 (MQ=255)                                  gCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCAtt                                                                                                              >  1:70001/1‑151 (MQ=255)                                     aaTTCCCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTc                                                                                                           <  2:86183/151‑1 (MQ=255)                                          ccccccCTGCGCCATTGACCTCGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCAGTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCTCACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTcaa                                                                                                         <  2:881727/148‑3 (MQ=255)                                                 tGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATc                                                                                               >  2:366384/1‑151 (MQ=255)                                                  gcgcCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATc                                                                                               >  1:232552/1‑150 (MQ=255)                                                     ccATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATcagca                                                                                           <  1:37327/151‑1 (MQ=255)                                                          gACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCgg                                                                                       <  2:155061/150‑1 (MQ=255)                                                           aCCAAGTACACCACGCCCGGCGGCAGTAAATAACCGACAATTTCAATTAGCCGCAGCACAGAAAGCGGGGTAAGACGTGTGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCg                                                                                        >  2:662311/1‑148 (MQ=255)                                                            ccACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCgg                                                                                       >  2:23147/1‑148 (MQ=255)                                                             cACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAg                                                                                   <  2:906257/151‑1 (MQ=255)                                                             cACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAg                                                                                   <  2:1062829/151‑1 (MQ=255)                                                                      cacGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACg                                                                            <  2:466117/149‑1 (MQ=255)                                                                       acGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGa                                                                         <  1:442485/151‑1 (MQ=255)                                                                         gCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGAt                                                                        >  2:1065907/1‑150 (MQ=255)                                                                         gCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATa                                                                       <  1:332110/151‑1 (MQ=255)                                                                           ccGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAAt                                                                     >  2:426642/1‑151 (MQ=255)                                                                            cggcggCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATc                                                                    >  1:290409/1‑151 (MQ=255)                                                                               cggcAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGc                                                                 >  1:677453/1‑151 (MQ=255)                                                                                      aaaTCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGGAAGACGTGCGGGTTTCAGCGCCACACAGGTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCccacc                                                           >  2:480047/1‑150 (MQ=255)                                                                                       aaTCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGTGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCcaccac                                                         >  1:321836/1‑151 (MQ=255)                                                                                       aaTCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCcaccac                                                         <  2:75843/151‑1 (MQ=255)                                                                                        aTCTCCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGCGAGCCATTTTCCATCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCccac                                                            <  2:453786/147‑1 (MQ=255)                                                                                             cGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTa                                                    >  2:893150/1‑150 (MQ=255)                                                                                              gACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTaa                                                   >  1:68633/1‑150 (MQ=255)                                                                                              gACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTaa                                                   <  2:68632/150‑1 (MQ=255)                                                                                                cAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACgg                                                >  2:48478/1‑151 (MQ=255)                                                                                                     tCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCAt                                           >  1:54948/1‑151 (MQ=255)                                                                                                      ccATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATg                                          <  1:728520/151‑1 (MQ=255)                                                                                                        aTTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGa                                        <  1:458673/151‑1 (MQ=255)                                                                                                          tAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGaa                                       <  1:404045/150‑1 (MQ=255)                                                                                                          tAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGaa                                       >  2:970712/1‑150 (MQ=255)                                                                                                           agcagcAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAAt                                     <  1:57977/151‑1 (MQ=255)                                                                                                           agcagcAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAAt                                     <  1:309522/151‑1 (MQ=255)                                                                                                           agcagaAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGCTCCACGGGATAATCTGCCCCACCACGCCTCACGGTTCATGGAAAt                                     >  2:188435/1‑151 (MQ=255)                                                                                                              agcagTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGAt                                  <  2:1004187/151‑1 (MQ=255)                                                                                                                cagTACAGAAAGCGGGGTAAGCCGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATa                                 <  2:141647/150‑1 (MQ=255)                                                                                                                cagTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGAt                                  >  2:873078/1‑149 (MQ=255)                                                                                                                    aCAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGcc                             >  1:630798/1‑150 (MQ=255)                                                                                                                      aGAAAGCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACg                          <  1:1028153/151‑1 (MQ=255)                                                                                                                           gCGGTGTAGGACGTTCGGGTTTCAGCGCCACACAGCTACCCCGCGCCAGCGCGGGAGCCATTTTCCAGTTCGCCATCAGCAGCGTGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCACGGAAAGGATAGGCCCCGGTTTc                     <  2:79213/151‑1 (MQ=255)                                                                                                                           gCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTc                     <  2:668520/151‑1 (MQ=255)                                                                                                                           gCGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTc                     <  2:935695/151‑1 (MQ=255)                                                                                                                            cGGGGTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCg                    <  2:94465/151‑1 (MQ=255)                                                                                                                             gggcTAAGACGGGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCACCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTACCGGGTCATGGAAATGCTATGCCACGGTTTCg                    >  2:1009964/1‑150 (MQ=255)                                                                                                                              gggTAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCt                  >  2:610975/1‑151 (MQ=255)                                                                                                                                 tAAGACGTGCGGGTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATc               >  2:659141/1‑151 (MQ=255)                                                                                                                                           gggTTTCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACt      <  2:282621/150‑1 (MQ=255)                                                                                                                                           aggTTTCAGAGCCACACAGTTGGCAGCCGCCAGCGGGGGAGCCTTTTTCCAGCTCGCCATCAGAAGCGGGAAGGTCAACGGGGTGATGTGCCACATCACGCCGAACGGGGCATGGACATGATAGGACACGGTTTCGCTATGACCTTCACTg     >  2:946552/2‑151 (MQ=255)                                                                                                                                              tttCAGCGCCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATc  >  1:66371/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                TTTGGCGATGCGTTTCGAGGTCGCCAGATATTCGCCAATTACCCCACCTGCGCCATTGACCACGTTCACCACGCCCGGCGGCAGTAAATCACCGACAATTTCCATTAGCAGCAGTACAGAAAGCGGGGTAAGACGTGCGGGTTTCAGCACCACACAGTTGCCCGCCGCCAGCGCGGGAGCCATTTTCCAGCTCGCCATCAGCAGCGGGAAGTTCCACGGGATAATCTGCCCCACCACGCCTAACGGTTCATGGAAATGATAGGCCACGGTTTCGCTATCAACTTCACTGATC  >  NC_000913/3755789‑3756080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑