Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	428,291	C→T	S215S (AGC→AGT)	secD →	SecYEG protein translocase auxillary subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,291	0	C	T	100.0%	225.2 / NA	60	S215S (AGC→AGT)	secD	SecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/29);  total (31/29)

CCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCA  >  NC_000913/428143‑428437                                                                                                                                                     |                                                                                                                                                   ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCT‑AAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGAt                                                                                                                                                 >  1:17604/1‑151 (MQ=255)     tGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCt                                                                                                                                              >  2:166244/1‑151 (MQ=255)      gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGc                                                                                                                                               <  2:1011966/149‑1 (MQ=255)         cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTc                                                                                                                                          >  2:540202/1‑151 (MQ=255)         cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTc                                                                                                                                          <  1:510217/151‑1 (MQ=255)          gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCt                                                                                                                                         <  1:970020/151‑1 (MQ=255)          gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCt                                                                                                                                         >  1:250005/1‑151 (MQ=255)            aaaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                        <  2:50591/150‑1 (MQ=255)            aaaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                        <  1:894104/150‑1 (MQ=255)                          cGGCCTGAGCAGCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTg                                                                                                                           <  2:466978/149‑1 (MQ=255)                             ccTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAatat                                                                                                                      <  1:577520/151‑1 (MQ=255)                               tGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGc                                                                                                                    >  1:318611/1‑151 (MQ=255)                                   cATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTg                                                                                                                <  2:494694/151‑1 (MQ=255)                                    aTCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgc                                                                                                               >  2:983751/1‑151 (MQ=255)                                      cACTTTCCGCGATGCTAACGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgc                                                                                                               >  2:554784/1‑149 (MQ=255)                                      cACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcag                                                                                                             <  2:943355/151‑1 (MQ=255)                                        cTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagc                                                                                                            >  1:256239/1‑150 (MQ=255)                                         tttCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagc                                                                                                            >  1:379746/1‑149 (MQ=255)                                           tacgcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCACCCGGACCTGGTGATTAGCAGCCAGGACAGGAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGGGCAGCAGa                                                                                                         >  2:736417/3‑150 (MQ=255)                                                gATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTa                                                                                                   <  1:376658/151‑1 (MQ=255)                                                     tAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATc                                                                                              >  1:584606/1‑151 (MQ=255)                                                     tAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATc                                                                                              >  2:812224/1‑151 (MQ=255)                                                     tAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATc                                                                                              >  2:473657/1‑151 (MQ=255)                                                      aaaGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAatat                                                                                               <  2:565705/149‑1 (MQ=255)                                                            cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCg                                                                                         <  2:577997/149‑1 (MQ=255)                                                            cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGt                                                                                        >  2:142457/1‑150 (MQ=255)                                                            cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTa                                                                                       <  1:781592/151‑1 (MQ=255)                                                                  gaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCgtg                                                                                 >  2:773345/1‑151 (MQ=255)                                                                  gaAGCAATTGCGAATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCACGGCAGCAACAAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCgtg                                                                                 >  2:635797/1‑151 (MQ=255)                                                                   aaGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCgtgt                                                                                >  1:503657/1‑151 (MQ=255)                                                                      ccATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTaaa                                                                             <  1:1006636/151‑1 (MQ=255)                                                                        aTTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAAcc                                                                           >  1:1064792/1‑151 (MQ=255)                                                                          tGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCa                                                                          <  2:732075/150‑1 (MQ=255)                                                                            cGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACt                                                                       <  1:613129/151‑1 (MQ=255)                                                                             gTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACtt                                                                      <  1:134458/151‑1 (MQ=255)                                                                                tCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCCCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggc                                                                   >  2:259466/1‑151 (MQ=255)                                                                                   gAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggcg                                                                  <  1:399934/149‑1 (MQ=255)                                                                                    aGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCACCCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtgg                                                                    <  2:273756/146‑1 (MQ=255)                                                                                           gcCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGcc                                                        >  1:441596/1‑151 (MQ=255)                                                                                              aTCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCggt                                                     <  2:641761/151‑1 (MQ=255)                                                                                                ccGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCggtgg                                                   <  1:414844/151‑1 (MQ=255)                                                                                                 cGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCggtgg                                                   >  2:310968/1‑150 (MQ=255)                                                                                                  ggACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGtt                                                 >  1:19500/1‑151 (MQ=255)                                                                                                    aCCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTc                                                >  2:266349/1‑150 (MQ=255)                                                                                                     ccTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTc                                                >  2:439526/1‑149 (MQ=255)                                                                                                        ggTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGt                                           <  2:641721/151‑1 (MQ=255)                                                                                                               agcagcCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCTTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTg                                    <  1:119844/151‑1 (MQ=255)                                                                                                                    ccAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGAc                               <  1:946779/151‑1 (MQ=255)                                                                                                                      aGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGCGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGGCAGGGTGCTGACCg                             >  2:1038781/1‑151 (MQ=255)                                                                                                                             caACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCgtt                      >  2:258845/1‑151 (MQ=255)                                                                                                                              aaCCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCgttg                     <  2:963229/151‑1 (MQ=255)                                                                                                                                   gCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGaa                <  1:623709/151‑1 (MQ=255)                                                                                                                                    cTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAAc               >  1:627089/1‑151 (MQ=255)                                                                                                                                      gcgtgcgGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACt              >  1:75527/1‑150 (MQ=255)                                                                                                                                      gcgtgcgGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTg             <  2:318587/151‑1 (MQ=255)                                                                                                                                        gtgcgGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGcc           >  2:740009/1‑151 (MQ=255)                                                                                                                                             gTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAgg        >  2:906266/1‑149 (MQ=255)                                                                                                                                               aaTGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTAtt    <  1:897987/151‑1 (MQ=255)                                                                                                                                               aaTGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTAtt    >  2:682676/1‑151 (MQ=255)                                                                                                                                                  gAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCa  <  2:995048/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCA  >  NC_000913/428143‑428437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑