| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,145,072 | C→T | P360S (CCG→TCG) | pflD → | putative glycine radical domain‑containing pyruvate formate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,145,072 | 0 | C | T | 94.8% | 179.4 / NA | 59 | P360S (CCG→TCG) | pflD | putative glycine radical domain‑containing pyruvate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (21/34); total (21/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTG > NC_000913/4144925‑4145218 | cgcTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGa < 2:52627/151‑1 (MQ=255) tCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAAcc > 2:552784/1‑151 (MQ=255) ccaccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAAc < 1:765323/149‑1 (MQ=255) ccaccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAAc < 1:499018/149‑1 (MQ=255) ccaccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGGGAACGTGCTTTCGTTCCTTTGCCTTTACGCCTATCAAAGCG‑TGCAATTACCGCAATCGaa > 2:906545/1‑148 (MQ=255) accaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGTACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCg < 2:229884/151‑1 (MQ=255) ccaGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCgg > 2:76521/1‑151 (MQ=255) caGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGc > 1:1004612/1‑151 (MQ=255) aGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCg < 1:879572/151‑1 (MQ=255) gTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGCCTTAGCGCGGTTAACGTGCTTTCGGTCCTTTGCCTTGACGCCTCTCAAAGCG‑TGCAATTACCGCAATCCAACCTCTGCg > 2:770001/1‑150 (MQ=255) gTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGa < 2:17618/141‑1 (MQ=255) gTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGa > 1:17618/1‑141 (MQ=255) cgcgcgTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGACCCTCGGCGTgcgc < 2:866509/151‑1 (MQ=255) aTTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGc < 2:903724/151‑1 (MQ=255) cGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAAAGCCTTg > 2:195504/1‑151 (MQ=255) ccGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGAt < 2:843179/151‑1 (MQ=255) tCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCC‑TTGCCTTGATGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGacac < 2:27749/150‑1 (MQ=255) cccGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACg < 2:719562/151‑1 (MQ=255) cGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGcc < 1:845906/151‑1 (MQ=255) cGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGcc < 2:245519/151‑1 (MQ=255) cGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGcc > 2:281768/1‑151 (MQ=255) gCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCt < 2:781894/151‑1 (MQ=255) tataCCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCtg > 2:865257/1‑150 (MQ=255) cGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGaaa < 1:895408/151‑1 (MQ=255) gCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAAccgcc < 1:132370/151‑1 (MQ=255) cggcggGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGa < 1:743983/150‑1 (MQ=255) ggcggGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGaa > 2:877581/1‑150 (MQ=255) ttAACCGAGAACGGCCGTAGCGTGGTGAACGTGCTTTCGTTCCTTGGCTTTGACGCCTACCAAGGCG‑TGCAATTCCCGCATTCAAACCTCGGCGTGCGCAATATCGCTTGGCACGACACGCCGTGCCTGATGAAAACCGCCGAAACCAtt < 2:608095/150‑1 (MQ=255) tAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTc > 2:443453/1‑150 (MQ=255) accgcagaACGGGCGTCGCGGGGGGAACGTGTTTTCGTCCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTCCCTCAACCGACCCGGGCCGTGCGCTCTAACGCCTTGATCGACACGCCGTTCTTGATGAACACCGCCGAACCCAtt < 2:39457/143‑1 (MQ=255) cagagaACGGCCGTAGCGCGGTGAACGTGTTTTCGTTCCTTGGCCTTGCCGCCTACCAAGGCT‑TGAAATCACCGCATTCGAACCTCGGCGTGCGCACTAACGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCtt < 2:830937/149‑1 (MQ=255) ggACGTAGCGCGGTGAACGTGCTTTCGTTGCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATGCCCGCAATCGAACCTCGGCGTGCGCACTAACGCTTTGAGCGACACGCCGTTCCTGATAAAAACCGCCGAAACCATTCGCTTCGGTACCg < 2:23722/151‑1 (MQ=255) ggACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTCCCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTAcc < 1:1060539/150‑1 (MQ=255) ggACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTCCCGCAATCGAACCGCGGCGTGCGCACTACCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTAcc < 1:562255/150‑1 (MQ=255) ggACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCg > 1:420176/1‑151 (MQ=255) gTAGCGCGGTTAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCgg < 1:164321/148‑1 (MQ=255) gcgcGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTcc > 2:199280/1‑151 (MQ=255) cgcgTTGAACTTGCTTTCGTTCCTTTCCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGACCCTCGGCGTGCGCCCTAACCCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTcc < 1:991652/150‑1 (MQ=255) cgGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTCCCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCg < 1:723764/149‑1 (MQ=255) ggTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACCCGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCa < 2:818044/150‑1 (MQ=255) gAACTTGCTTTCGTTCCTTTGCTTTGACGCCTATC‑AAGCGTTGAAATTGCCGCATGCGAACCTCGCCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAATCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAAt < 1:910053/150‑1 (MQ=255) aaCGTGCTTGCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTGGGCGTGCGCCCTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATGCGCTTCGGTACCGGTATTCCGCAAAt < 2:561932/149‑1 (MQ=255) tGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTa > 1:8589/1‑150 (MQ=255) tttCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGAAATTACCGCAATCGACCCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTa < 2:1053892/147‑1 (MQ=255) gTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa > 1:52683/1‑150 (MQ=255) ttCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa > 2:697160/1‑149 (MQ=255) ttCCTTGGCTTTGACGCCTATCAAAGCG‑GGCAATTCCCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGa < 1:868690/149‑1 (MQ=255) tCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgt > 1:628212/1‑151 (MQ=255) ccTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtg < 1:562186/151‑1 (MQ=255) ttGCCTTGACGCCTATCAAGGCG‑TGCAATTACCGCAATCGACCCCGGGCGTGCGCACTAACTCTTGGATCGCCACGCCGTTCTTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtgg < 1:836003/149‑1 (MQ=255) tGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAgtggtgg > 1:290323/1‑151 (MQ=255) tccTATAAAAGCG‑TGCAATTACCGAAACCGAACCTCGGCGTGCGCTCTAACGCTTTGACCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGc < 2:116753/148‑1 (MQ=255) ccTATCAAAGCG‑TGCAATTCCCGCAATCGACCCCCGGCGTGCGCACTACCGCTTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGt < 2:470084/150‑1 (MQ=255) cTATCAAAGCG‑TGCAATTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTc > 1:477872/1‑151 (MQ=255) g‑TGCAATTCCCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGt < 1:717415/150‑1 (MQ=255) aaTTACCGCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGt > 1:937028/1‑151 (MQ=255) tcccGCAATCGACCCTCGGCGTGCGCCCTAACGCCTGGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGttt < 1:462328/148‑1 (MQ=255) ccGCAACCGACCCTCGGCGTGCGCCCTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGt < 2:995382/146‑1 (MQ=255) gCAATCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTg > 2:230236/1‑151 (MQ=255) | CGCTCCACCAGTAGCGCGCGTTATTTCGCCGGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCTTTGCCTTGACGCCTATCAAAGCG‑TGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTG > NC_000913/4144925‑4145218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |