Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,795	A→G	T851A (ACC→GCC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,795	0	A	G	100.0%	210.9 / NA	57	T851A (ACC→GCC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

GCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAA  >  NC_000913/4183651‑4183942                                                                                                                                                 |                                                                                                                                                    gCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTg                                                                                                                                               <  2:1016769/151‑1 (MQ=255)     tCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACAt                                                                                                                                           >  1:31385/1‑151 (MQ=255)        tGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATccc                                                                                                                                        >  2:292580/1‑151 (MQ=255)         gCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCg                                                                                                                                       >  1:781785/1‑151 (MQ=255)          ccGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCg                                                                                                                                       >  1:691199/1‑150 (MQ=255)            gTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCTCATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCGGGGTCCGGAAGAGATCGCCGCTGACATCCCGAAc                                                                                                                                    <  2:186679/151‑1 (MQ=255)             tGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACg                                                                                                                                   >  2:279000/1‑151 (MQ=255)               gAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTg                                                                                                                                 >  2:498236/1‑151 (MQ=255)                      tACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGaa                                                                                                                           <  2:1061609/150‑1 (MQ=255)                       aCAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCTCCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAg                                                                                                                          <  2:724150/150‑1 (MQ=255)                        cAACTTCGAAGACTCCATCCGCAGATCCGAGCGTGTTGTTCCTGAACACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGGCACCAAGCGGGGTCGGGAAGAGATCGCCGCTGACATCCCGTTCGTGGGTGATGCt                                                                                                                        <  2:731908/151‑1 (MQ=255)                         aaCTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGCCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTg                                                                                                                       >  2:307669/1‑151 (MQ=255)                             tCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTgcgc                                                                                                                    <  1:441476/150‑1 (MQ=255)                              cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGctc                                                                                                                  <  2:188451/151‑1 (MQ=255)                              cGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGct                                                                                                                   <  2:125387/150‑1 (MQ=255)                               gAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGctct                                                                                                                 <  1:116468/151‑1 (MQ=255)                                      ccATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAAc                                                                                                           <  1:213294/150‑1 (MQ=255)                                      ccATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACt                                                                                                          <  2:345911/151‑1 (MQ=255)                                        aTCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTgg                                                                                                        >  1:713536/1‑151 (MQ=255)                                            tCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAt                                                                                                      >  2:1053844/1‑149 (MQ=255)                                             cGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATg                                                                                                     <  1:745879/149‑1 (MQ=255)                                                  ccGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCgg                                                                                              >  1:183385/1‑151 (MQ=255)                                                  ccGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCg                                                                                               <  2:917425/150‑1 (MQ=255)                                                     aGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTAt                                                                                           <  2:173390/151‑1 (MQ=255)                                                             gttCAGGAAGACCGGTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGATGACATCCCGAACGTGGGTGAAGCTGCGCTCTTCAAACTGGATGAATCCGGTATCGTTTAc                                                                                    <  2:323703/150‑1 (MQ=255)                                                                cAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACAtt                                                                                 <  1:13420/150‑1 (MQ=255)                                                                     aGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCg                                                                           <  1:6931/151‑1 (MQ=255)                                                                      gACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCgg                                                                          <  1:843855/151‑1 (MQ=255)                                                                       aCCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCg                                                                           >  2:564624/1‑149 (MQ=255)                                                                        ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGa                                                                         >  1:1024747/1‑150 (MQ=255)                                                                        ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGa                                                                         >  2:376318/1‑150 (MQ=255)                                                                        ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGa                                                                         >  1:879532/1‑150 (MQ=255)                                                                              caccacCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAcc                                                                  >  2:931295/1‑151 (MQ=255)                                                                              caccacCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAcc                                                                  >  1:119281/1‑151 (MQ=255)                                                                                   ccatccatATTCAGGAACTGGCGTGTGTGCCCCTTCACTCCAAGCTGGGTCCGAAAGAGATCGCCGCTGACATCCCGAACGTGCGTGGAGCTGCGCTCTCCATACGGGATGAATCCGGTATCGTTTACATTGGTGCGGTAGTGACCGGTg                                                              <  2:346663/150‑1 (MQ=255)                                                                                    caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGt                                                               <  2:667603/148‑1 (MQ=255)                                                                                    caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTCGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTgg                                                             >  2:959955/1‑150 (MQ=255)                                                                                     aTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCg                                                           >  2:823517/1‑151 (MQ=255)                                                                                     aTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCg                                                           <  2:651182/151‑1 (MQ=255)                                                                                                ggAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggtt                                                <  2:644519/151‑1 (MQ=255)                                                                                                   aCTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttggt                                             <  1:143220/151‑1 (MQ=255)                                                                                                        cGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTggtaaggt                                        >  1:832288/1‑151 (MQ=255)                                                                                                             gtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACg                                    <  1:1044815/150‑1 (MQ=255)                                                                                                               gtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCg                                 >  2:1012607/1‑151 (MQ=255)                                                                                                                 cccGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGa                                >  1:609795/1‑150 (MQ=255)                                                                                                                  ccGTGACACCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGaa                               >  1:1068489/1‑150 (MQ=255)                                                                                                                       acacCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGa                         <  2:707583/151‑1 (MQ=255)                                                                                                                         acCAAGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGaaa                       <  2:526857/151‑1 (MQ=255)                                                                                                                             aGCTGGGTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCa                   <  1:761243/151‑1 (MQ=255)                                                                                                                                tGGGTCCGGAAGAGATCGCCGCTGACATCCCGGACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGc                 >  2:613679/1‑150 (MQ=255)                                                                                                                                 gggTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTg               <  2:613270/151‑1 (MQ=255)                                                                                                                                 gggTCCGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTg               <  2:869466/151‑1 (MQ=255)                                                                                                                                     ccGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGTAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAccc           >  2:265738/1‑151 (MQ=255)                                                                                                                                     ccGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAccc           >  1:160602/1‑151 (MQ=255)                                                                                                                                      cGGAAGAGATCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAcccc          >  2:898086/1‑151 (MQ=255)                                                                                                                                            agaTCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGCGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTAAGCTGACCCCaga       >  2:999403/1‑148 (MQ=255)                                                                                                                                              aTCGCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGaaa  <  1:220390/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    GCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAA  >  NC_000913/4183651‑4183942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑