| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,204,350 | A→G | Q344R (CAG→CGG) | zraR → | fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,204,350 | 0 | A | G | 85.2% | 142.5 / ‑0.6 | 54 | Q344R (CAG→CGG) | zraR | fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/23); minor base C (8/0); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCC > NC_000913/4204201‑4204493 | tCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCa < 2:862367/150‑1 (MQ=255) gCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGc < 2:831023/151‑1 (MQ=255) cAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCT‑CTGCTGGCTGGCCATTTTTTGCAGCGCTTT‑GCCGAGCGGAATCGC‑CA‑GG‑CGGGAAAAAGGTTTATGCCCCCGGCg > 1:30519/1‑150 (MQ=255) ggATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATg < 1:487561/151‑1 (MQ=255) ctctACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGAAATTCCT‑CTGCTTGCTGGCCATTTTTTGCAGCGCCTT‑TCCGAGGGGAATCGC‑AAGGG‑GGGGAAAAGGTTTTAGCCCCCGGGGGAgggt > 2:552935/1‑149 (MQ=255) ctactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGa < 1:703285/147‑1 (MQ=255) ctactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATc < 2:22862/149‑1 (MQ=255) ctactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGAAATCCCC‑CTTCTGGGCGGCCACTTTTTTCCGCGCTTT‑TGCGGGGGGTATCTC‑AA‑GGAGGGGAAAAGGTTTT‑CTCCCCCGGCg > 2:1001042/1‑142 (MQ=255) actaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGGTGGCCATTTTTTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTggt > 1:440416/1‑149 (MQ=255) actaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCt < 1:1393/148‑1 (MQ=255) tCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGGTGGGCCTTTTCTGCAGCGCGTT‑TCCGCGCGGAAACCC‑AA‑AG‑CGGGAAAAGGGTTTACGCCCCGGGCGATGGGTCTGTTGAtt > 2:251653/1‑151 (MQ=255) cGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTc < 1:271312/151‑1 (MQ=255) gCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCa < 1:40073/151‑1 (MQ=255) ccTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGGTATTCCT‑CTGCTGGGTGGCCCATTTTTGCCGCGCCTT‑TCCGGGCGGAATCGC‑AA‑AG‑CGGGCAAAGGGTTTTCGCCCCCGGCGGTGGGTCTGGTGATTCAt > 1:763389/1‑151 (MQ=255) gAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTa < 1:124266/150‑1 (MQ=255) gAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCC‑CTGCTGGGTGGCCATTTTCTTCAGCGCTTT‑TGCGAGCGTAATCGC‑CA‑GG‑CGGTAAAAGGGTTTTCGCCCCCGGCGATGGAGCTGTTGTTTCAtt > 2:854049/1‑149 (MQ=255) gAATGTGGTGGCGATTGAAGTACCATCGCTGCGGAAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTAc < 1:141584/151‑1 (MQ=255) gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGAAATCACC‑CCGCCGGCTGGCCATTTTTTTCAAGGCTTT‑TGCCAGCGTAAACGCAAA‑GG‑GGGAAAAAGGTTTTA‑GCCCCCGGGGAGGGGTCTGTTTGTTCAtt > 1:377898/1‑145 (MQ=255) ggtggCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑AG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTg < 1:985678/150‑1 (MQ=255) gCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCC‑CCGCTTGCTGGGCCCTTTTTTCCGCGCTTTGGCCGAG‑GGAATCCC‑AA‑AG‑CGGGAAAAGGGTTTAAGCCCCGGGCGGTGGGTCTTTTGTTTTAATTCGACTTGCCgg > 2:552569/1‑151 (MQ=255) gATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGa < 1:303584/151‑1 (MQ=255) ttGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCCTTTTTTTCACCGCTTT‑GCCGAGCGTAATCGC‑AA‑AG‑CGGGAAAAGGTTTTTCGCCCCCGGCGGTGGGTCTGTTGTTTCATTTAGACTGGCCgggga > 1:605954/1‑148 (MQ=255) aGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATAtt < 2:841144/151‑1 (MQ=255) aGTACCATCGCTGCGGCAACGGCGGAAAGATATACCTCCTGC‑GGCTGGCCATTCTCTTCTGCACCTT‑GGCCACCCCAATCCG‑GA‑CG‑CGGGGAAAGGATTTTCGCCCCCGGCGGTGGTTCTTTTTGTTTATTACGAcacgcc > 2:384046/1‑136 (MQ=255) cATCGCTGCGGCAACGGCGGGAAGACATCCCT‑CCGCTTGCTGGCCCTTTTTTTCACCGCTTT‑TCCGAGCGGAAACCC‑AA‑GG‑CGGGAAAAGGGTTTATGCCCCGGGGGATGGGTCTGTTTATTCATTACGACTGGCCGGGGAATATTCGTg > 1:430443/1‑150 (MQ=255) aTCGCTGCGGCAACGGCGGGGAGAGAACCCT‑CTTCCGGCTGGCCCATTTTTGCAGCGGCTT‑GCCGAGAGGAAACGC‑AA‑AG‑GGGGAAAAGGGTTTACGCCCCGGGCGGTGGGGCTGTTGATTCATTTAGAACGGCCGGGGAATATTtgtggg > 2:96128/1‑149 (MQ=255) gCTGCGGCAACGGCGGGAAGAAATCCCC‑CTCCAGGCCGGCCCTTTTCTGCCGACCTCT‑GTCCGGCGCAAAACC‑AA‑AG‑CGGGAAAAAGTTTTTAGCCCCCGGCGAGGGGTCTGTTGTTTCATTACTAACGGCCCGGAAAGATTTGGGAGc > 2:972412/1‑149 (MQ=255) tGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGGTGGCCAATTTTTGCAGCGCTTT‑GCCGGGCGGAAACGC‑AA‑AG‑CGGGAAAAGGGTTTACGCCCCGGGCGATGGATCTGTTGGTTTATTTAGACTGGGCGGGGAATAATTGTGGGCTgg > 2:358393/1‑150 (MQ=255) cGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGAc < 1:414407/123‑1 (MQ=255) ggCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGaaaa < 1:131787/151‑1 (MQ=255) cggcggGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCgg > 1:94605/1‑151 (MQ=255) tgggAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTgg > 2:116350/2‑151 (MQ=255) atatCCCT‑CTCCTGGCTGGCCATTTTCTGCACCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGCAATATTCGTGAGCTGGAAACCGCGGTGGAACggg > 2:1049548/1‑150 (MQ=255) tatCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGc > 2:968249/1‑150 (MQ=255) atCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAg < 1:879052/151‑1 (MQ=255) ggctggcCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTgctgct < 1:738652/150‑1 (MQ=255) ggctggcCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGctgctg > 1:958418/1‑151 (MQ=255) ctggcCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAc > 1:941393/1‑151 (MQ=255) agcgatttgatgaaGCGCGTT‑GCCGAGCGGAATCAC‑AA‑GG‑CGGGAAACGGCTTTACGCCCAGGGCGATGGATCTGCTGATTCACTACGACTGGCCGGGAAATATTCGCGAGCTGGAAAACGCGGTGGAATGGACAGTGGGGCTGCTGACCg > 2:1048366/14‑151 (MQ=255) cATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgggg < 1:94445/151‑1 (MQ=255) tttCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAat > 1:233553/1‑151 (MQ=255) ttCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAat < 2:263097/150‑1 (MQ=255) cTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatat > 2:232788/1‑150 (MQ=255) tGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatatat > 1:984900/1‑151 (MQ=255) gCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgc < 2:877814/150‑1 (MQ=255) gcGAGCGTAATCGC‑AA‑GG‑CGGTGAAAGGGTTTACGCCCCGGGCGATGGATCTGTGGTTTCATTACGACTGGCAGGGAAATATTCGTGAGCTGGAAAAACCGGTGGAACGGGCAGTGGGGCTGCTGACCGGGGAATATATTTCCGAAcgc > 2:217603/2‑149 (MQ=255) ccGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGa < 1:461906/151‑1 (MQ=255) gCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt < 2:457859/150‑1 (MQ=255) gCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTg < 1:700773/151‑1 (MQ=255) tAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCg > 2:560354/1‑151 (MQ=255) aTCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCt < 1:25770/151‑1 (MQ=255) c‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCg > 1:738910/1‑151 (MQ=255) aaaaGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGt > 1:552816/1‑151 (MQ=255) tACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATc > 2:459923/1‑151 (MQ=255) aCGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATcc > 1:429233/1‑151 (MQ=255) | TCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT‑CTGCTGGCTGGCCATTTTCTGCAGCGCTTT‑GCCGAGCGTAATCGC‑AA‑GG‑CGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCC > NC_000913/4204201‑4204493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |