| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,228,771 | A→G | E347E (GAA→GAG) | yjbB → | putative Na+/Pi‑cotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,228,771 | 0 | A | G | 98.3% | 211.4 / NA | 59 | E347E (GAA→GAG) | yjbB | putative Na+/Pi‑cotransporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/33); minor base C (0/1); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGGTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGG > NC_000913/4228623‑4228916 | cACGGTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCa < 1:482433/151‑1 (MQ=255)cACGGTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCa < 2:284413/151‑1 (MQ=255) gTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAgatg > 2:1066412/1‑151 (MQ=255) tttGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAgatga < 1:785610/150‑1 (MQ=255) tttGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAgatga < 1:204041/150‑1 (MQ=255) ttGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAgatgat < 2:177957/150‑1 (MQ=255) cAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGaa < 1:689409/151‑1 (MQ=255) aaaaCGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATgg > 2:352279/1‑148 (MQ=255) aaaaCGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCGGGATGTCAGCGCGCTGGAAACGCACGCGCTTGCTCTGGCGAACGCCGCTCGC‑GAAACCCTGCGCATTGGCGACGCCGTGGAGCAGATGATgg > 2:788774/1‑148 (MQ=255) aaaCGAATATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATAAGCACACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAgg > 2:249225/1‑151 (MQ=255) aaCGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAggg > 2:351034/1‑151 (MQ=255) aCGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGt < 1:1066420/151‑1 (MQ=255) gATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTg < 1:853425/151‑1 (MQ=255) tCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATa > 2:683787/1‑149 (MQ=255) gcgATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGt < 1:197215/151‑1 (MQ=255) gATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGa < 1:711717/151‑1 (MQ=255) cGGAACTGGATACCCCGCTATGGCCTAAACGTCTGGATGTCGGCGCGCTGGATACGCGCACGCGTGCTCTGGCGAGCGCCGCGCGC‑GAAACCCGGCGCATTGGCGACGCCGTGGAGGAGCTGATTGAAGGGTTGAATAAAGTGATGCACg < 2:916463/150‑1 (MQ=255) cGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACgg < 1:39579/151‑1 (MQ=255) ggAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGt > 1:1001427/1‑151 (MQ=255) ggAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGt > 2:737350/1‑151 (MQ=255) aaCTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTg > 2:1069323/1‑150 (MQ=255) aaCTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGa < 1:848975/151‑1 (MQ=255) cccAGCTTCGGCCTAAACATCTGGATGTCAGCGCGCTGGATGCGCCCACGCGTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACgg < 2:351990/149‑1 (MQ=255) cccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCa < 2:65890/151‑1 (MQ=255) aGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAgg > 2:381887/1‑150 (MQ=255) tACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGgaga > 1:185226/1‑150 (MQ=255) aCGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGgaga < 1:767339/149‑1 (MQ=255) cGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAag < 1:342090/151‑1 (MQ=255) ccTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGc > 2:603151/1‑151 (MQ=255) tAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTg > 1:468476/1‑151 (MQ=255) cATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGt > 2:990554/1‑150 (MQ=255) tCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTa > 2:534686/1‑149 (MQ=255) cAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGc > 2:966128/1‑147 (MQ=255) aGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgatg > 2:395321/1‑151 (MQ=255) cgcTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGatat < 2:856462/151‑1 (MQ=255) gcTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATc > 1:724430/1‑151 (MQ=255) tGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATc > 2:428529/1‑149 (MQ=255) gATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACg < 2:397777/151‑1 (MQ=255) aCGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGtt < 2:992676/150‑1 (MQ=255) cGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTtct < 2:1046689/151‑1 (MQ=255) cccACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTctct < 1:307228/151‑1 (MQ=255) ttGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGc > 2:340152/1‑151 (MQ=255) ctGGCGAACGCCGCGGGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGACATCACCGTTCTCTACACCGCCAt < 2:341000/149‑1 (MQ=255) gCGACCGCCGC‑CGCGGAAGCCCGGCGCATTGGCGCCGCCATGGACGGGATGAGGGAGGGTTTAAAAAAAGTGAGGCGTGGTGCGCCGCGGCAGAAAAAAGGGCGGCGTAAGCTGGCAGATGATATAAACGTTCTCTACACCGCCAtt < 2:546588/147‑1 (MQ=255) gccgcGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTAt < 2:378388/150‑1 (MQ=255) gcgcgc‑gAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTgg > 2:215395/1‑151 (MQ=255) gggAACCCCTGCGCTTTGGCCACGCCGTGGAGCAGATGATGGAAGGGTGGAATAAATTGAGCCACGGTGCGCCACGGCAGGAAAAAGAGCTGCGAGATCGGGCAGATGATATAAGCGTTCTCTCCGCCGCCATTAAGCTGTATCTGgcgc < 2:500180/148‑1 (MQ=255) c‑gAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCgg > 1:1051666/1‑151 (MQ=255) gAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCgg > 1:739685/1‑150 (MQ=255) aaCCCTGCGCCTTGGCGGCGCCATGGGGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGAGGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGa < 2:91919/149‑1 (MQ=255) gcgcATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGTAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCaaaa < 1:272128/151‑1 (MQ=255) gcgcATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACGCCGCCATTAAGCTGTATCTGGCGCGGATGCCaaa < 1:367325/150‑1 (MQ=255) gcgcATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCaaa < 1:125088/150‑1 (MQ=255) gcATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCaaa > 1:434142/1‑148 (MQ=255) tgggCGACGCAATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCAACGGCAGGAGAAAGGGCTGGGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAaagaag < 2:78499/148‑1 (MQ=255) ggCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGTTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAagag < 1:764366/150‑1 (MQ=255) ggCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAagag < 2:413120/150‑1 (MQ=255) gACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTgg < 1:351978/151‑1 (MQ=255) aTGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCggagg > 1:915915/1‑151 (MQ=255) | CACGGTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGC‑GAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGG > NC_000913/4228623‑4228916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |