Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,443,730	C→T	T450M (ACG→ATG)	tamA →	translocation and assembly module for autotransporter export, outer membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,443,730	0	C	T	100.0%	269.7 / NA	71	T450M (ACG→ATG)	tamA	translocation and assembly module for autotransporter export, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/34);  total (37/34)

TGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT  >  NC_000913/4443583‑4443876                                                                                                                                                    |                                                                                                                                                   tGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATgc                                                                                                                                                  >  2:839079/1‑150 (MQ=255)     gCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATgctggg                                                                                                                                              <  2:549454/150‑1 (MQ=255)          ccTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGAt                                                                                                                                        >  1:464201/1‑151 (MQ=255)             ggggCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGAtt                                                                                                                                       <  1:811924/149‑1 (MQ=255)             ggggCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTg                                                                                                                                      >  1:151539/1‑150 (MQ=255)             ggggCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTg                                                                                                                                      <  2:966728/150‑1 (MQ=255)                       gCAACGCTACTCTATCGACTACTCCAACACGGACTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGAt                                                                                                                           >  1:408329/1‑151 (MQ=255)                           cGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCg                                                                                                                       <  1:20258/151‑1 (MQ=255)                                      cGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTAcc                                                                                                             <  1:352593/150‑1 (MQ=255)                                          tactCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCgg                                                                                                        >  1:284874/1‑151 (MQ=255)                                              ccAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGAt                                                                                                      <  2:884028/149‑1 (MQ=255)                                                aaCACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGc                                                                                                  >  2:777334/1‑151 (MQ=255)                                                 acacGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCg                                                                                                 >  2:353728/1‑151 (MQ=255)                                                    cGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGttt                                                                                              <  1:888733/151‑1 (MQ=255)                                                     ggCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttc                                                                                             >  2:234803/1‑151 (MQ=255)                                                      gCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttct                                                                                            >  1:958428/1‑151 (MQ=255)                                                       ccTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttctt                                                                                           <  1:234828/151‑1 (MQ=255)                                                       ccTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttctt                                                                                           <  1:612542/151‑1 (MQ=255)                                                          ggggTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttcttc                                                                                          <  2:857219/149‑1 (MQ=255)                                                           gggTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTttcttc                                                                                          <  2:547933/148‑1 (MQ=255)                                                           gggTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGcc                                                                                       >  1:794244/1‑151 (MQ=255)                                                             gTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCgg                                                                                     <  2:591548/151‑1 (MQ=255)                                                                cAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCgggg                                                                                   <  2:768139/150‑1 (MQ=255)                                                                     gTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGAcc                                                                             <  1:697900/151‑1 (MQ=255)                                                                     gTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGAcc                                                                             <  2:954268/151‑1 (MQ=255)                                                                        gATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGc                                                                           >  2:515300/1‑150 (MQ=255)                                                                        gATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGc                                                                           >  2:888374/1‑150 (MQ=255)                                                                        caTTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGc                                                                           >  2:820529/2‑150 (MQ=255)                                                                          tttCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAg                                                                         >  2:901808/1‑150 (MQ=255)                                                                          tttCTCCGTTTTCCAGGCGCAGAACGTCTGGAGCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCAGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGACCTGCGTTTCTTCGCCGGGGGCGACCGCAGt                                                                        >  2:47534/1‑151 (MQ=255)                                                                           ttCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGt                                                                        >  1:923704/1‑150 (MQ=255)                                                                            tctcCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGCCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGGAt                                                                      >  2:79061/1‑151 (MQ=255)                                                                              tcCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTc                                                                    >  1:819383/1‑151 (MQ=255)                                                                               ccGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCg                                                                   >  2:985946/1‑151 (MQ=255)                                                                                cGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGt                                                                  <  2:969305/151‑1 (MQ=255)                                                                                       cAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCtaca                                                           <  1:777376/151‑1 (MQ=255)                                                                                        aGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCtaca                                                           >  1:42745/1‑150 (MQ=255)                                                                                           cgcAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCtacaaata                                                       <  2:453371/151‑1 (MQ=255)                                                                                              aGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCtacaaataca                                                     >  1:691148/1‑150 (MQ=255)                                                                                              aGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCGGGGCTGGATTGAAGCCGGTGATTTCGGCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCtacaaatac                                                      <  2:559020/149‑1 (MQ=255)                                                                                                 aCGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATc                                                 <  2:418069/151‑1 (MQ=255)                                                                                                 aCGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATc                                                 >  2:905752/1‑151 (MQ=255)                                                                                                 aCGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATc                                                 >  1:26912/1‑151 (MQ=255)                                                                                                 aCGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATc                                                 >  1:957418/1‑151 (MQ=255)                                                                                                   gtgtGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAGAGTTCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTa                                               <  2:425567/148‑1 (MQ=255)                                                                                                   gTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCt                                                >  1:160778/1‑150 (MQ=255)                                                                                                    tCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTAt                                              >  1:653833/1‑151 (MQ=255)                                                                                                       ggATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGc                                           <  2:508997/151‑1 (MQ=255)                                                                                                          tCCGCACACTTTTCGAACGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTTAAACCGGTGTTTTCGACAAAGTACCGCCGCATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATAGATCGCt                                          <  2:344971/149‑1 (MQ=255)                                                                                                          tCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTcc                                        >  1:640687/1‑151 (MQ=255)                                                                                                           ccGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTcc                                        <  1:530563/150‑1 (MQ=255)                                                                                                           ccGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTcc                                        <  2:26911/150‑1 (MQ=255)                                                                                                             gCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGa                                      >  1:534004/1‑150 (MQ=255)                                                                                                                   tGTACGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGc                               >  1:591651/1‑151 (MQ=255)                                                                                                                      aCGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                            <  1:416450/151‑1 (MQ=255)                                                                                                                      aCGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCa                             >  1:990151/1‑150 (MQ=255)                                                                                                                       cGATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                           >  2:218765/1‑151 (MQ=255)                                                                                                                        gATCGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACg                          >  1:1031335/1‑151 (MQ=255)                                                                                                                           cGCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                        <  1:282619/150‑1 (MQ=255)                                                                                                                            gCCATCGTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGa                      <  1:24494/151‑1 (MQ=255)                                                                                                                               atggTTTTGTCACACGCGGCATGTTGGGCTGGATTGAAACCGGTGATTTCGATAAAGTACCGCCGGATCTGCGGTTGTTCGCCGGGGAGGACCGCAGTATTAGTGGCAACAAATTTAAACCTGTTGCTCCGACATACGCCTACGGTGACCt                   >  2:2857/4‑151 (MQ=255)                                                                                                                                  gTTTTGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGa                 <  2:884966/150‑1 (MQ=255)                                                                                                                                    tttGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                        <  2:273891/141‑1 (MQ=255)                                                                                                                                    tttGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                        >  1:273915/1‑141 (MQ=255)                                                                                                                                    tttGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa               <  2:958379/150‑1 (MQ=255)                                                                                                                                    tttGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaa                >  1:929181/1‑149 (MQ=255)                                                                                                                                     ttGTTACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAAGGTGACCTGAAAgg             >  2:995090/1‑151 (MQ=255)                                                                                                                                        ttACACGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgggg           >  1:1011238/1‑150 (MQ=255)                                                                                                                                          acacGCGGCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt        <  2:815427/151‑1 (MQ=255)                                                                                                                                               cgGCATGCTGGGCTGGATTCACCCAGCTTACTGCGACAAATTCCCGCCCGATCTGCTTTTCTTCGCCGTCGGCGATCGCAGTATGCGTGTCTACAAATACAAATCTATCGCTCCGCAACACCCCAACGTTGACCTCAAAGGGGccagga     <  2:11242/149‑5 (MQ=255)                                                                                                                                                ggCATGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt  <  1:9862/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TGATGCCAACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACGGCCTGGGGTTCAGATGTCGATTTCTCCGTTTTCCAGGCGCAGAACGTCTGGATCCGCACACTGTACGATCGCCATCGTTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT  >  NC_000913/4443583‑4443876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑