Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,500,381	T→C	pseudogene (154/942 nt)	yjgX ←	pseudogene fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,500,381	0	T	C	100.0%	199.3 / NA	53	pseudogene (154/942 nt)	yjgX	pseudogene fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/33);  total (20/33)

GTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGTACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTATCATATA  >  NC_000913/4500239‑4500524                                                                                                                                               |                                                                                                                                                gtgtCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAgg                                                                                                                                         <  1:1029922/151‑1 (MQ=255)      aTGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTc                                                                                                                                    >  1:1048818/1‑151 (MQ=255)           cccccAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAg                                                                                                                                 <  2:1004611/149‑1 (MQ=255)             cccAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGtat                                                                                                                             >  1:365669/1‑151 (MQ=255)             cccAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGtat                                                                                                                             <  2:868807/151‑1 (MQ=255)                 gTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATc                                                                                                                         <  2:617258/151‑1 (MQ=255)                    ttCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGt                                                                                                                       <  1:207417/150‑1 (MQ=255)                     tCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTc                                                                                                                      <  1:1040095/150‑1 (MQ=255)                          gATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCaataa                                                                                                                 <  1:888860/150‑1 (MQ=255)                           aTTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCaata                                                                                                                  <  2:125053/148‑1 (MQ=255)                             tGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCaataaata                                                                                                              <  2:231984/150‑1 (MQ=255)                                  atCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCgg                                                                                                        >  1:41273/1‑151 (MQ=255)                                        caTGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGcca                                                                                                  <  2:611714/151‑2 (MQ=255)                                        caTGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCg                                                                                                  <  1:446291/151‑1 (MQ=255)                                             gCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTAtt                                                                                              <  1:327973/150‑1 (MQ=255)                                                gTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGTTATAATCGTCAATAAATAAACCGGGTGCCGTATTCa                                                                                            <  1:505129/149‑1 (MQ=255)                                                gTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCa                                                                                            <  2:160208/149‑1 (MQ=255)                                                  aTCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCa                                                                                        <  1:828137/151‑1 (MQ=255)                                                         ttttGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGtgtg                                                                                 >  2:32130/1‑151 (MQ=255)                                                           ttGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAACCCGGGTGCCGTATTCAGCCACGgtgt                                                                                  <  2:935777/148‑1 (MQ=255)                                                                gcCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGtggttggt                                                                           <  2:553889/150‑1 (MQ=255)                                                                gcCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGtggttggt                                                                           >  2:277298/1‑150 (MQ=255)                                                                  cAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACg                                                                        >  2:954849/1‑151 (MQ=255)                                                                      gcCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGAt                                                                    <  2:992486/151‑1 (MQ=255)                                                                       ccaccaCATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATa                                                                   <  1:900224/151‑1 (MQ=255)                                                                         accacATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGt                                                                 >  1:704675/1‑151 (MQ=255)                                                                          ccacATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTc                                                                >  1:406808/1‑151 (MQ=255)                                                                            acaTTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCAt                                                              <  2:371928/151‑1 (MQ=255)                                                                             caTTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCAt                                                              >  1:523531/1‑150 (MQ=255)                                                                             caTTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCAt                                                              <  2:1023927/150‑1 (MQ=255)                                                                               ttgttgCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATac                                                            >  1:67920/1‑150 (MQ=255)                                                                                tgttgCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATacac                                                          >  2:770466/1‑151 (MQ=255)                                                                                tgttgCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATacac                                                          >  2:764419/1‑151 (MQ=255)                                                                                tgttgCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATaca                                                           <  2:953419/150‑1 (MQ=255)                                                                                     ccGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGAc                                                      >  1:97138/1‑150 (MQ=255)                                                                                        gAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACatat                                                  >  2:804875/1‑151 (MQ=255)                                                                                          aTCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATaa                                                <  1:625610/151‑1 (MQ=255)                                                                                            cAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATc                                              <  2:406782/151‑1 (MQ=255)                                                                                              gggTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATccc                                            <  1:1052159/151‑1 (MQ=255)                                                                                                  tCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGATGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTTACATATAATCCCTgc                                         <  2:1054/150‑1 (MQ=255)                                                                                                     gCTAATAGTCATAAATCAGTGTCCGGCTAAGCGAAGCCTCGGCACTGGACGCTGTCGAGGTATAATCGTCAATCAATAAACCGTGTGCCGTATTCAGTCACTGTGTGTCTGGTACCTGATGGTGAGACCCTGACATATATTCCTTgtg                                        <  2:793110/148‑3 (MQ=255)                                                                                                        cATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCacac                                   <  2:465015/150‑1 (MQ=255)                                                                                                         aTAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGcaca                                    <  2:155314/148‑1 (MQ=255)                                                                                                         aTAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACActc                                 <  2:270538/151‑1 (MQ=255)                                                                                                           aGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACtctc                               >  2:965879/1‑151 (MQ=255)                                                                                                           aGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACtctc                               >  2:975648/1‑151 (MQ=255)                                                                                                            gTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACtctc                               >  1:585297/1‑150 (MQ=255)                                                                                                                              gCTCAGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATc            >  1:425496/1‑151 (MQ=255)                                                                                                                                  aGGGAAGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACTGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTAt        >  1:257386/1‑151 (MQ=255)                                                                                                                                   ggaaaGACACTGCACTGAAGGTTGTCGATGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTAAGCGATAGTCAGACACTGACATATAATCCCTGCACACACTCTCACAAGTGACAATAACAATCatat        <  2:999233/147‑1 (MQ=255)                                                                                                                                      aaGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTATCata    <  1:662287/151‑1 (MQ=255)                                                                                                                                      aaGACACTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTATCat     >  1:291541/1‑150 (MQ=255)                                                                                                                                         acacTGCACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTATCAtata  <  2:368696/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                GTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGCCACCACATTGTTGCCGGAATCAGGGTTCTGCTCATAGTCATAAATCAGTGTCCGGCTCAGGGAAGACACTGTACTGGAGGCTGTCGAGGTATAATCGTCAATAAATAAACCGGGTGCCGTATTCAGCCACGGTGTGGTTGGTACGGGATAGTCATACACTGACATATAATCCCTGCGCACACTCTCACCAGTGACAATAACAATCGTATCATATA  >  NC_000913/4500239‑4500524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑