Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	479,603	C→T	V106I (GTT→ATT)	maa ←	maltose O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	479,603	0	C	T	100.0%	203.2 / NA	53	V106I (GTT→ATT)	maa	maltose O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/28);  total (25/28)

TATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAACGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAATATAAGCCTCTGTCAC  >  NC_000913/479462‑479750                                                                                                                                              |                                                                                                                                                    tATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGc                                                                                                                                            >  1:870246/1‑151 (MQ=255) tATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGc                                                                                                                                            >  2:310182/1‑151 (MQ=255)   tCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGcc                                                                                                                                           >  1:713773/1‑150 (MQ=255)   tCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGATTACCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGcc                                                                                                                                           >  1:242354/1‑150 (MQ=255)    cACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCa                                                                                                                                          <  1:690813/150‑1 (MQ=255)    cACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCa                                                                                                                                          >  1:739989/1‑150 (MQ=255)      ccAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAAc                                                                                                                                        >  2:868079/1‑150 (MQ=255)       cAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACAt                                                                                                                                      <  2:929922/151‑1 (MQ=255)             tCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGtt                                                                                                                                >  1:720446/1‑151 (MQ=255)                 acCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATc                                                                                                                             >  1:509400/1‑150 (MQ=255)                   cAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCAcc                                                                                                                          >  1:711187/1‑151 (MQ=255)                   cAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCAcc                                                                                                                          <  1:338539/151‑1 (MQ=255)                             gACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGCTGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAAt                                                                                                                 >  2:555034/1‑150 (MQ=255)                                 gcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGc                                                                                                            <  2:693644/151‑1 (MQ=255)                                  cgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGc                                                                                                            >  1:682976/1‑150 (MQ=255)                                                cAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCa                                                                                             <  2:914728/151‑1 (MQ=255)                                                 aGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCAc                                                                                            >  1:105427/1‑151 (MQ=255)                                                  gACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACg                                                                                           <  2:116493/151‑1 (MQ=255)                                                   aCGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGc                                                                                          >  2:91076/1‑151 (MQ=255)                                                    cGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGc                                                                                          >  1:913475/1‑150 (MQ=255)                                                       tattaCCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAAt                                                                                       <  2:956342/150‑1 (MQ=255)                                                        attaCCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATc                                                                                      <  1:968192/150‑1 (MQ=255)                                                            ccGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAg                                                                                  <  1:153774/150‑1 (MQ=255)                                                               aTGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTgg                                                                              <  1:288880/151‑1 (MQ=255)                                                                tGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGc                                                                             >  1:472798/1‑151 (MQ=255)                                                                     aCGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGaaaa                                                                        <  1:579972/151‑1 (MQ=255)                                                                     aCGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGaaaa                                                                        <  1:437806/151‑1 (MQ=255)                                                                     aCGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGaaa                                                                         >  1:389754/1‑150 (MQ=255)                                                                              cccAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGAGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTAGTAc                                                               <  2:110923/151‑1 (MQ=255)                                                                                cAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCg                                                             <  1:318516/151‑1 (MQ=255)                                                                                       gCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGaaaa                                                       >  2:581807/1‑150 (MQ=255)                                                                                               aTTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGatat                                                >  2:89123/1‑149 (MQ=255)                                                                                               aTTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTtata                                               <  1:36064/150‑1 (MQ=255)                                                                                               aTTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTtata                                               <  2:686209/150‑1 (MQ=255)                                                                                                 tACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGc                                             <  1:738336/150‑1 (MQ=255)                                                                                                  aCGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGc                                             >  2:444543/1‑149 (MQ=255)                                                                                                   cGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCAt                                          <  1:929947/151‑1 (MQ=255)                                                                                                      gCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAg                                        <  2:920001/150‑1 (MQ=255)                                                                                                      gCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAg                                        <  1:754666/150‑1 (MQ=255)                                                                                                      gCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGt                                       <  1:162454/151‑1 (MQ=255)                                                                                                           aGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTcaca                                   <  1:468471/150‑1 (MQ=255)                                                                                                           aGGGTCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTcac                                    >  2:379390/1‑149 (MQ=255)                                                                                                               tCGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGAGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATAAAGCATCACGCAATAGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCg                                >  2:344848/1‑149 (MQ=255)                                                                                                                cGATGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGaa                              <  1:636001/150‑1 (MQ=255)                                                                                                                  aTGGGATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCGCGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACg                           <  2:690128/151‑1 (MQ=255)                                                                                                                      gATGTGTTGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTgg                       <  1:752551/151‑1 (MQ=255)                                                                                                                          tgtgGCCGTGTAGATATGAATGCCTGTTGCCAACATCCAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGAAATCGAAGTTGGCGAGAAAATTATTACCGAGAAAAATATTATAGCCATAGTCACAGCGAAACGTTGtctc                    >  2:316662/1‑150 (MQ=255)                                                                                                                            ttGCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAat                 >  1:67082/1‑151 (MQ=255)                                                                                                                              gCCGTGTAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAatat               <  2:107199/151‑1 (MQ=255)                                                                                                                                   gtAGATATGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAATATAAGcc          <  2:109519/151‑1 (MQ=255)                                                                                                                                        tatGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAATATAAGCCTCTGt     >  1:396984/1‑151 (MQ=255)                                                                                                                                          tGAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCATACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACATCTAAACGTTGGCTCAATATAAGCCTCTGt     <  2:79895/149‑1 (MQ=255)                                                                                                                                           gAATGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAATATAAGCCTCTGTCAc  >  1:989891/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    TATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTACGTGCTACAGGGTCGATGGGATGTGTTGCCGTGTAGATATGAACGCCTGGTGCCAACATACAGTTATCACCGATGCGAATAGGGCAGACATCAAGCATCACGCAATCGAAGTTGGCGAAAAAATTATTACCGAGAAAAATGTTATAGCCATAGTCACAGCGAAACGTTGGCTCAATATAAGCCTCTGTCAC  >  NC_000913/479462‑479750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑