Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	496,931	A→G	E604E (GAA→GAG)	htpG →	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	496,931	0	A	G	100.0%	228.8 / NA	62	E604E (GAA→GAG)	htpG	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/33);  total (29/33)

TGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGT  >  NC_000913/496784‑497078                                                                                                                                                    |                                                                                                                                                    tGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAgcgc                                                                                                                                                  >  1:11853/1‑151 (MQ=255)   cggcggGCCAGAAAGTGCCCGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCgg                                                                                                                                                >  2:552159/1‑151 (MQ=255)     gcggGCCACAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGCACTGGTGAAACGTGCGGCAGATACTGAAGATGACGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGc                                                                                                                                               >  2:208232/1‑150 (MQ=255)       gggCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACg                                                                                                                                            <  1:28165/151‑1 (MQ=255)          ccAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCTCGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTg                                                                                                                                         >  1:355775/1‑151 (MQ=255)          ccAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTg                                                                                                                                         <  1:797363/151‑1 (MQ=255)            aGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTgg                                                                                                                                        >  2:1044812/1‑150 (MQ=255)             gAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGa                                                                                                                                       >  1:590711/1‑150 (MQ=255)                  tGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATcc                                                                                                                                 >  1:107320/1‑151 (MQ=255)                   gCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCg                                                                                                                                >  2:668248/1‑151 (MQ=255)                    ccAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGa                                                                                                                               <  1:932360/151‑1 (MQ=255)                     cAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGa                                                                                                                               >  1:461976/1‑150 (MQ=255)                       gAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATGCTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAAcc                                                                                                                            <  2:19346/151‑1 (MQ=255)                         aGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTg                                                                                                                          <  1:478826/151‑1 (MQ=255)                          gTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGt                                                                                                                         >  2:399387/1‑151 (MQ=255)                          gTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGt                                                                                                                         >  1:931362/1‑151 (MQ=255)                            gAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGttt                                                                                                                       >  2:686167/1‑151 (MQ=255)                                     cTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTcgtcgt                                                                                                              >  1:211832/1‑151 (MQ=255)                                       tCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATtcgtc                                                                                                                <  2:243254/147‑1 (MQ=255)                                       tCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTa                                                                                                             <  1:734711/150‑1 (MQ=255)                                          aaCTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGa                                                                                                          >  1:888382/1‑150 (MQ=255)                                            cTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAAcc                                                                                                       >  1:707785/1‑151 (MQ=255)                                                aCCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAgct                                                                                                   <  2:211807/151‑1 (MQ=255)                                                    ggATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGctgctg                                                                                               >  1:343889/1‑151 (MQ=255)                                                     gATCACGTGCTGGTGAAACGTGCGGCAGATACTGTAAATGAAGCGAAGTTCAGCTAGTGGGTACAACTGTTTTTGGATCAGGCGCTGATGGCACAGCGCGGCATGCTGGAAGATCCGATTCTCTGTATTGGTAGAATGAACAAgcagctc                                                                                               <  2:34055/150‑2 (MQ=255)                                                     gATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTgg                                                                                              >  2:1038502/1‑151 (MQ=255)                                                        cACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGttt                                                                                           <  1:652217/151‑1 (MQ=255)                                                           gTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCt                                                                                        >  1:683505/1‑151 (MQ=255)                                                             aCTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTg                                                                                       >  1:89600/1‑150 (MQ=255)                                                                ggTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt                                                                                   <  1:1038557/151‑1 (MQ=255)                                                                 gTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTa                                                                                  <  1:813035/151‑1 (MQ=255)                                                                 gTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTa                                                                                  <  2:1024354/151‑1 (MQ=255)                                                                    aaaCGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATg                                                                               <  2:530707/151‑1 (MQ=255)                                                                     aaCGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGc                                                                              <  2:163670/151‑1 (MQ=255)                                                                       cGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCg                                                                            <  1:787396/151‑1 (MQ=255)                                                                            ggCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGa                                                                       <  2:945860/151‑1 (MQ=255)                                                                              cAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGAcc                                                                     >  2:775073/1‑151 (MQ=255)                                                                                gATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCtt                                                                   <  2:784729/151‑1 (MQ=255)                                                                                   aCTGAAGATGAAGCGAAGTTCAGCGAGTGGATAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCg                                                                 <  2:383354/150‑1 (MQ=255)                                                                                    cTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCg                                                                 <  2:707166/149‑1 (MQ=255)                                                                                          aTGACGCGAAGTTCGGCATTTGGATGGAACTGCTGCTGGGTCTTGCGCTGCTGGCAAAGCGCGTGTCGCTGGATGCTGCGAACCTGTTTATTCGTCGTATAAGCCATCTACTGCTTACCTTATTTAATGCCTTGTAACCTTCGTGTCATcc                                                         <  2:423367/151‑1 (MQ=255)                                                                                                 gAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCAttt                                                  >  1:788566/1‑151 (MQ=255)                                                                                                 gAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCAttt                                                  <  1:998323/151‑1 (MQ=255)                                                                                                  aaGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCAtttt                                                 >  2:468967/1‑151 (MQ=255)                                                                                                       cAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttcttt                                            >  2:82183/1‑151 (MQ=255)                                                                                                       cAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttcttt                                            <  2:310020/151‑1 (MQ=255)                                                                                                       cAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttcttt                                            <  1:12207/151‑1 (MQ=255)                                                                                                       cAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTGTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttcttt                                            >  2:906228/1‑151 (MQ=255)                                                                                                        aGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttctttt                                           <  1:88719/151‑1 (MQ=255)                                                                                                              tGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTcatcat                                     >  1:799794/1‑151 (MQ=255)                                                                                                               gggTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTcatcat                                     <  2:779752/150‑1 (MQ=255)                                                                                                                   aGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGc                                 <  2:89598/150‑1 (MQ=255)                                                                                                                    gAACTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCAc                               >  1:332247/1‑151 (MQ=255)                                                                                                                     aaCTGCTGCTGGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACt                              <  2:287532/151‑1 (MQ=255)                                                                                                                       ctgctgctgGATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACttt                            >  2:69664/1‑151 (MQ=255)                                                                                                                            gctgAATCAGGCGCTGGTGGCCGGGCGTGGCTCGCTGGAATATGCGAACCTGTTTATTCGACGTCTGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACTTTCGTGTCATCCGGCAGTTTTCTTTTCCTCAGCTGCACtt                             <  2:1038295/145‑1 (MQ=255)                                                                                                                                gATCAGGCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAAtt                   <  1:338976/151‑1 (MQ=255)                                                                                                                                     ggCGCTGCTGGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTAtctc              >  1:130178/1‑151 (MQ=255)                                                                                                                                          tgctgGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACAAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCAt         >  2:46842/1‑151 (MQ=255)                                                                                                                                           gctgGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCAt         <  2:595186/150‑1 (MQ=255)                                                                                                                                            ctgGCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCAtt        >  1:224100/1‑150 (MQ=255)                                                                                                                                              ggCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTaa      <  2:429475/150‑1 (MQ=255)                                                                                                                                              ggCAGAGCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTaa      <  2:230369/150‑1 (MQ=255)                                                                                                                                                 agagCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGt  <  2:1060658/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGCGGCGGGCCAGAAAGTGCCAGAAGTGAAATACATCTTCGAACTGAACCCGGATCACGTACTGGTGAAACGTGCGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGT  >  NC_000913/496784‑497078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑