Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 615,301 T→C L382P (CTG→CCG)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913615,3010TC98.2% 194.3 / ‑3.6 56L382P (CTG→CCG) entFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (26/29);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCTGTTGTGCGGCGATGT  >  NC_000913/615154‑615448
                                                                                                                                                   |                                                                                                                                                   
gTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGtt                                                                                                                                                  <  1:732622/151‑1 (MQ=255)
    cAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCg                                                                                                                                              <  1:653421/151‑1 (MQ=255)
        gggCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGAt                                                                                                                                           <  1:493254/150‑1 (MQ=255)
        gggCGAGCGGCAGGTGATGAACCGCTGCTTGGTCCGGTACTCAATATCAAGGGATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCACACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGGACTGGCCCCGTTCCCGGAt                                                                                                                                           >  2:1051317/1‑150 (MQ=255)
              gCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACg                                                                                                                                    <  1:213524/151‑1 (MQ=255)
              gCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACg                                                                                                                                    >  2:495837/1‑151 (MQ=255)
                ggCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATCCCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGt                                                                                                                                  <  2:26475/151‑1 (MQ=255)
                      tgatgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTg                                                                                                                            >  1:58484/1‑151 (MQ=255)
                       gatgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGTATGTACACGGTGATTTg                                                                                                                            <  1:248813/150‑1 (MQ=255)
                        atgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAg                                                                                                                          <  2:162607/151‑1 (MQ=255)
                         taacccGCTGGTTGGTCCGGTACTCAATATCGAGGGATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCAAACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGt                                                                                                                         >  2:992432/5‑151 (MQ=255)
                             ccGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTg                                                                                                                     <  1:82467/151‑1 (MQ=255)
                              cGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTg                                                                                                                     >  1:174094/1‑150 (MQ=255)
                                  gTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGAt                                                                                                                 <  1:1028499/150‑1 (MQ=255)
                                   tttGGTCCGGTACTCAATATCAAGGAATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATcc                                                                                                               >  2:850487/1‑151 (MQ=255)
                                            gTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATa                                                                                                      <  1:1026238/151‑1 (MQ=255)
                                              aCTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATaaa                                                                                                    <  1:774584/151‑1 (MQ=255)
                                               cTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGcc                                                                                                          <  1:195004/144‑1 (MQ=255)
                                               cTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGcc                                                                                                          >  2:195001/1‑144 (MQ=255)
                                                  aaTATCAAGGTATTTGATTACCCACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAg                                                                                                 >  2:630227/1‑150 (MQ=255)
                                                  aaTATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGc                                                                                                <  1:390066/151‑1 (MQ=255)
                                                    tatCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGt                                                                                              >  2:88535/1‑151 (MQ=255)
                                                    tatCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGt                                                                                              >  1:987878/1‑151 (MQ=255)
                                                     atCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGt                                                                                              <  1:809412/150‑1 (MQ=255)
                                                        aaGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACg                                                                                          >  1:178715/1‑151 (MQ=255)
                                                        aaGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACg                                                                                          >  2:1026867/1‑151 (MQ=255)
                                                             aTTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAg                                                                                     <  1:29112/151‑1 (MQ=255)
                                                              tttGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGc                                                                                    >  2:467301/1‑151 (MQ=255)
                                                               ttGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGc                                                                                    <  2:296104/150‑1 (MQ=255)
                                                                 gATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCaa                                                                                 <  1:123840/151‑1 (MQ=255)
                                                                     aCCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGt                                                                              <  2:568690/150‑1 (MQ=255)
                                                                     aCCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGt                                                                              <  1:338930/150‑1 (MQ=255)
                                                                              atatTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGc                                                                     >  1:44963/1‑150 (MQ=255)
                                                                               tatTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCAt                                                                   >  1:434249/1‑151 (MQ=255)
                                                                                  tCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGGGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                                                >  1:25772/1‑151 (MQ=255)
                                                                                      gATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGaa                                                             <  1:26823/150‑1 (MQ=255)
                                                                                           tCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTg                                                       >  2:584830/1‑151 (MQ=255)
                                                                                              ggCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTGCCCGGATGTACACGGTGATTTGAGTATTGCGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAAGCCAGCATGCTGAACGCCTGaaa                                                    <  2:403229/151‑1 (MQ=255)
                                                                                                  cAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGc                                                >  1:759921/1‑151 (MQ=255)
                                                                                                           aCCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGccc                                       >  1:825104/1‑151 (MQ=255)
                                                                                                               tGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGtt                                   >  1:624895/1‑151 (MQ=255)
                                                                                                                ggCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAgg                                    <  2:599397/149‑2 (MQ=255)
                                                                                                                 gCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTcg                                 <  2:174093/151‑1 (MQ=255)
                                                                                                                 gCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTcg                                 <  1:232242/151‑1 (MQ=255)
                                                                                                                 gCAACCGGTCCGGTTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTcg                                 >  1:818247/1‑151 (MQ=255)
                                                                                                                      cggtccggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTcgccgc                             <  2:692182/150‑1 (MQ=255)
                                                                                                                       ggtccggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCgg                           <  1:437547/151‑1 (MQ=255)
                                                                                                                       ggtccggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCgg                           >  2:662656/1‑151 (MQ=255)
                                                                                                                        gtccggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGa                          <  1:170500/151‑1 (MQ=255)
                                                                                                                          ccggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGAt                         <  1:927651/150‑1 (MQ=255)
                                                                                                                           cggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATcc                       >  2:398572/1‑151 (MQ=255)
                                                                                                                           cggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATcc                       >  2:161540/1‑151 (MQ=255)
                                                                                                                            ggtTAATGACCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCg                      >  2:710543/1‑151 (MQ=255)
                                                                                                                                    aCCTTGAACTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCTTGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCtgtt              >  1:855490/1‑151 (MQ=255)
                                                                                                                                          aaCTGGCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCTGTTGTgcgg        <  2:427822/151‑1 (MQ=255)
                                                                                                                                               gCCCCGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCTGTTGTGCGGCGATg   <  2:723769/151‑1 (MQ=255)
                                                                                                                                                ccccGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCTGTTGTGCGGCGATGt  >  1:901523/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGGATCCGGCGCTGTTGTGCGGCGATGT  >  NC_000913/615154‑615448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: