Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,682	A→G	T424A (ACA→GCA)	ftsI →	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,682	0	A	G	100.0%	227.7 / NA	62	T424A (ACA→GCA)	ftsI	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/28);  total (34/28)

CGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGG  >  NC_000913/92536‑92826                                                                                                                                                   |                                                                                                                                                 cGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGGCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCAcc                                                                                                                                              >  2:379912/1‑151 (MQ=255)    tagtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCAt                                                                                                                                            <  2:732856/150‑1 (MQ=255)    tagtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCAt                                                                                                                                            <  2:93729/150‑1 (MQ=255)       tagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTAc                                                                                                                                         >  2:951303/1‑150 (MQ=255)       tagATACTTACTCACGTTTTCGACTGGGAAAAGCGACCAATCTGGGGTTGGTCGGCGAACGCAGTGGCTTATATTCTCAAAAACAACGGTGGGCTGACATAGAGAGGACCACCTTCTCTCTCGGCTACGGGCTAATGGCAGCACCATTAc                                                                                                                                         >  2:456117/1‑150 (MQ=255)        agATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACa                                                                                                                                        <  1:423952/150‑1 (MQ=255)          atccttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGCAGCACCATTACAGtt                                                                                                                                     >  2:202010/4‑151 (MQ=255)          aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGtt                                                                                                                                     <  1:903456/151‑1 (MQ=255)            acttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTa                                                                                                                                    <  1:970056/150‑1 (MQ=255)            acttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTa                                                                                                                                    <  1:5345/150‑1 (MQ=255)             cttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAgc                                                                                                                                  >  2:159070/1‑151 (MQ=255)               tactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAgcgc                                                                                                                                >  1:335669/1‑151 (MQ=255)                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGcgcg                                                                                                                               <  2:399137/151‑1 (MQ=255)                 ctCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGcgcg                                                                                                                               >  1:57760/1‑150 (MQ=255)                   cACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGt                                                                                                                            >  1:574442/1‑151 (MQ=255)                   cACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGt                                                                                                                            >  1:116683/1‑151 (MQ=255)                              cTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGCAGCACCATTACAGTTAGCGCGAGTCTACGCAACt                                                                                                                  >  2:803727/1‑150 (MQ=255)                                gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATc                                                                                                               <  1:18396/151‑1 (MQ=255)                                                 tGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAttt                                                                                               >  1:614144/1‑150 (MQ=255)                                                  ggggTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTAt                                                                                             <  2:862911/151‑1 (MQ=255)                                                   gggTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTAt                                                                                             >  2:167568/1‑150 (MQ=255)                                                        ggTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCa                                                                                       <  2:898526/151‑1 (MQ=255)                                                           cGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACt                                                                                     <  1:376790/150‑1 (MQ=255)                                                           cGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTGTCGGCTACGGGCTAATGGTAGCCCCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACt                                                                                     <  2:417814/150‑1 (MQ=255)                                                              agaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCg                                                                                 >  1:936212/1‑151 (MQ=255)                                                               gaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCg                                                                                 >  2:1004944/1‑150 (MQ=255)                                                                aaCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGAt                                                                               >  1:581406/1‑151 (MQ=255)                                                                          cTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGtt                                                                     >  2:753944/1‑151 (MQ=255)                                                                           ttATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTg                                                                    >  1:257924/1‑151 (MQ=255)                                                                            tataTCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGtt                                                                     >  2:405532/1‑149 (MQ=255)                                                                             atatCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAc                                                                  >  1:857533/1‑151 (MQ=255)                                                                              tatCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcc                                                                 >  1:944172/1‑151 (MQ=255)                                                                                   tCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATAACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCACACAGTCGATTACCAACGTTGACCCCCCg                                                            >  2:297962/1‑151 (MQ=255)                                                                                    cAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccccc                                                             <  1:7602/149‑1 (MQ=255)                                                                                    cAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCg                                                            >  2:294404/1‑150 (MQ=255)                                                                                         acaacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCTCCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTccc                                                      <  2:375406/151‑1 (MQ=255)                                                                                          caacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCg                                                     >  1:541839/1‑151 (MQ=255)                                                                                          caacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCg                                                     >  1:742862/1‑151 (MQ=255)                                                                                           aacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCgg                                                    >  2:440505/1‑151 (MQ=255)                                                                                                       aCATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtgt                                           <  1:515813/148‑1 (MQ=255)                                                                                                       aCATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCtt                                        >  2:387177/1‑151 (MQ=255)                                                                                                        cctAGAGAGCCCCACCTTCTCTCCCGCCTACCAGCTAACGGTAGCACCATTACAGTTAGCGCGCGTCTACGCAACTATCTCCAGCTACGGCATTTACCGCCCACTGTCGATTACCAAAGTTGACCCCCCGTTTCCCGTTGCTCGCGTCtt                                        >  2:4732/3‑150 (MQ=255)                                                                                                        cATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTc                                       <  2:989883/151‑1 (MQ=255)                                                                                                           agagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTccc                                     <  2:964356/150‑1 (MQ=255)                                                                                                           agagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCg                                    >  2:985662/1‑151 (MQ=255)                                                                                                             agagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGa                                  <  2:395570/151‑1 (MQ=255)                                                                                                              gagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGa                                  >  2:893056/1‑150 (MQ=255)                                                                                                                   ccACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCAt                            <  1:350176/151‑1 (MQ=255)                                                                                                                    cACCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCAtt                           >  1:987758/1‑151 (MQ=255)                                                                                                                     aCCTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTg                          >  2:544665/1‑151 (MQ=255)                                                                                                                      ccTTCTCTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGt                         <  1:814907/151‑1 (MQ=255)                                                                                                                         tctcTTTCGGCTACGGTCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCg                      >  2:79675/1‑151 (MQ=255)                                                                                                                         tctcTTTCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTcc                       <  2:639153/150‑1 (MQ=255)                                                                                                                              ttCGGCTACGGGCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCAc                   <  2:614110/149‑1 (MQ=255)                                                                                                                                       gggCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCata        <  1:83933/151‑1 (MQ=255)                                                                                                                                       gggCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCat         >  1:143054/1‑150 (MQ=255)                                                                                                                                        ggCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCatat       <  1:743021/151‑1 (MQ=255)                                                                                                                                        ggCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCatat       <  1:692711/151‑1 (MQ=255)                                                                                                                                        ggCTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCatat       <  1:406520/151‑1 (MQ=255)                                                                                                                                          cTAATGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatg      >  1:97144/1‑150 (MQ=255)                                                                                                                                            aaTGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATatgatg   <  1:856091/151‑1 (MQ=255)                                                                                                                                             aTGGTAGCACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATgg  <  2:590097/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGG  >  NC_000913/92536‑92826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑