Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,982	C→T	P97S (CCG→TCG)	entB →	isochorismatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,982	0	C	T	100.0%	175.9 / NA	45	P97S (CCG→TCG)	entB	isochorismatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

TGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAG  >  NC_000913/627841‑628128                                                                                                                                              |                                                                                                                                                   tGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCAGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTg                                                                                                                                            >  1:99590/1‑150 (MQ=255)          atgGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGATCGc                                                                                                                                  <  1:18598/151‑1 (MQ=255)              aGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGa                                                                                                                              <  1:388404/151‑1 (MQ=255)               gCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGaa                                                                                                                             <  2:246666/151‑1 (MQ=255)                      atagcgAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACACTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACa                                                                                                                           >  2:750070/4‑146 (MQ=255)                      aTCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCaaa                                                                                                                      <  1:542101/151‑1 (MQ=255)                        cgcgAATATTGCTGCACTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCaaa                                                                                                                      >  2:1045336/1‑149 (MQ=255)                               atTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGgtggtg                                                                                                              <  2:149837/150‑1 (MQ=255)                                    tgcgctgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGc                                                                                                        >  2:454707/1‑151 (MQ=255)                                        ctgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTg                                                                                                     >  2:676445/1‑150 (MQ=255)                                         tgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGAc                                                                                                   >  1:531642/1‑151 (MQ=255)                                                ccactgataaCAGCATAATATCCCGGCTTATCACACCGCTCAGCCGACGGTGCAGAGCGACGAAGAATGGGCCCTGTTGGATGTTATGTGTGGGTCTCTCCTAACCCGGCCGCCGGATTCGCAATTTGTGGTGGATCGCCTGACGcc                                                                                                >  2:683347/9‑147 (MQ=255)                                                 tactGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATg                                                                                           >  1:3581/1‑151 (MQ=255)                                                 tactGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATg                                                                                           >  2:436697/1‑151 (MQ=255)                                                   ctGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGcc                                                                                         >  2:519595/1‑151 (MQ=255)                                                          cAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGaca                                                                                  <  1:76413/151‑1 (MQ=255)                                                            gCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACg                                                                                >  1:63417/1‑151 (MQ=255)                                                                aaTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGc                                                                            <  1:272316/151‑1 (MQ=255)                                                                aaTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGc                                                                            >  2:143974/1‑151 (MQ=255)                                                                   atCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTg                                                                          <  1:660408/150‑1 (MQ=255)                                                                     cccGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTg                                                                       <  1:136259/151‑1 (MQ=255)                                                                       cGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGa                                                                      >  1:970344/1‑150 (MQ=255)                                                                       cGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGa                                                                      >  2:1067457/1‑150 (MQ=255)                                                                          tttATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGCGCTGGTGAAGTg                                                                  >  2:855626/1‑151 (MQ=255)                                                                                 cacCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTAc                                                           >  1:189649/1‑151 (MQ=255)                                                                                  acCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGACTGACGTCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACa                                                          <  1:131434/151‑1 (MQ=255)                                                                                   ccGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTAc                                                           >  2:1011367/1‑149 (MQ=255)                                                                                      cccAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAgcgc                                                      >  2:762875/1‑151 (MQ=255)                                                                                            cGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTc                                                 >  1:439498/1‑150 (MQ=255)                                                                                                   gcagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtct                                         <  1:215502/151‑1 (MQ=255)                                                                                                    cagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtctc                                        >  1:668719/1‑151 (MQ=255)                                                                                                    cagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtctc                                        <  1:605951/151‑1 (MQ=255)                                                                                                     agagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTTTcc                                       >  1:779861/1‑151 (MQ=255)                                                                                                           aTGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTgg                                  >  1:513237/1‑150 (MQ=255)                                                                                                                gATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCaaa                            <  1:990659/151‑1 (MQ=255)                                                                                                                 aTCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAAt                           <  1:167969/151‑1 (MQ=255)                                                                                                                  tCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATg                          >  2:215861/1‑151 (MQ=255)                                                                                                                           gTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAag                   <  1:182464/149‑1 (MQ=255)                                                                                                                           gTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAag                   <  1:155796/149‑1 (MQ=255)                                                                                                                               aaTGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTg             <  2:941067/151‑1 (MQ=255)                                                                                                                                      tgtgGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTa       >  1:616379/1‑150 (MQ=255)                                                                                                                                        tgGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAAcc    >  1:274806/1‑151 (MQ=255)                                                                                                                                         gggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAAcc    <  1:535089/150‑1 (MQ=255)                                                                                                                                         gggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCa   <  2:181527/151‑1 (MQ=255)                                                                                                                                          ggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAg  <  1:379376/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   TGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAG  >  NC_000913/627841‑628128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑