Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,179	A→G	N429S (AAC→AGC)	cstA →	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,179	0	A	G	100.0%	175.6 / NA	46	N429S (AAC→AGC)	cstA	carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/26);  total (20/26)

GTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGT  >  NC_000913/631031‑631325                                                                                                                                                     |                                                                                                                                                   gTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGcc                                                                                                                                                  <  2:1023385/151‑1 (MQ=255)    tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGcccg                                                                                                                                                >  2:975044/1‑148 (MQ=255)      cGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGGGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGAtt                                                                                                                                             >  2:465323/1‑151 (MQ=255)           gTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCa                                                                                                                                         <  2:1044742/150‑1 (MQ=255)             atcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGc                                                                                                                                       <  1:647424/150‑1 (MQ=255)               catcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGaa                                                                                                                                    <  2:846906/151‑1 (MQ=255)                  cGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTg                                                                                                                                 <  1:940249/151‑1 (MQ=255)                      ccGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTg                                                                                                                             >  1:424030/1‑151 (MQ=255)                      ccGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTg                                                                                                                             >  2:47956/1‑151 (MQ=255)                         ggCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAAc                                                                                                                          >  2:354242/1‑151 (MQ=255)                              gtATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTc                                                                                                                      <  1:459258/150‑1 (MQ=255)                                   ttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCa                                                                                                                 <  1:536409/150‑1 (MQ=255)                                      ccATGAACAGCCCGATGGCGGGGCTGGCTCCGGCAGGGACGGCGGCTGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTc                                                                                                             >  2:634346/1‑151 (MQ=255)                                        aTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTccc                                                                                                           <  2:240575/151‑1 (MQ=255)                                        aTGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTccc                                                                                                           >  2:770423/1‑151 (MQ=255)                                           aaCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTg                                                                                                        <  2:94098/151‑1 (MQ=255)                                                      ggCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTgcg                                                                                             <  2:450897/151‑1 (MQ=255)                                                        cGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGcc                                                                                           <  1:228601/151‑1 (MQ=255)                                                              tGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGc                                                                                      >  2:281503/1‑150 (MQ=255)                                                                gCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTgg                                                                                   <  1:149618/151‑1 (MQ=255)                                                                     gtcAGGGACGGCGGATGGGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTg                                                                              >  2:50351/3‑151 (MQ=255)                                                                      gCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTgg                                                                             >  1:26423/1‑151 (MQ=255)                                                                              ggcggATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGcc                                                                     >  1:991174/1‑151 (MQ=255)                                                                                 ggATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTAc                                                                  <  1:371019/151‑1 (MQ=255)                                                                                            cTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACgg                                                       >  1:28531/1‑151 (MQ=255)                                                                                              tCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACgg                                                       <  1:93156/149‑1 (MQ=255)                                                                                                 gCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGgcgcg                                                   <  1:546828/150‑1 (MQ=255)                                                                                                 gCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGgcgc                                                    <  1:570387/149‑1 (MQ=255)                                                                                                             gtgAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGGGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCat                                        >  2:119865/1‑149 (MQ=255)                                                                                                                   aGCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggatg                                >  2:129482/1‑151 (MQ=255)                                                                                                                    gCTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGAtgt                               >  1:507354/1‑151 (MQ=255)                                                                                                                     cTGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGAtgtg                              >  1:634698/1‑151 (MQ=255)                                                                                                                      tGGGGCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCCACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTgg                             >  2:522726/1‑151 (MQ=255)                                                                                                                        gggCTTTAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCg                           <  1:761826/151‑1 (MQ=255)                                                                                                                              tAGCATTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTg                      <  1:929401/150‑1 (MQ=255)                                                                                                                                  aTTACACCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATc                 <  2:371838/151‑1 (MQ=255)                                                                                                                                     acacCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCAc               >  2:134887/1‑150 (MQ=255)                                                                                                                                     acacCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACt              <  1:4614/151‑1 (MQ=255)                                                                                                                                     acacCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACt              <  1:1027757/151‑1 (MQ=255)                                                                                                                                      cacCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACtt             <  2:992386/151‑1 (MQ=255)                                                                                                                                       acCAGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGGATCACtt             >  2:488163/1‑150 (MQ=255)                                                                                                                                          aGATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATTGATGTGGCGTTCTGGTATCACTTCGcc         <  1:212023/151‑1 (MQ=255)                                                                                                                                           gATACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCa        <  2:242754/151‑1 (MQ=255)                                                                                                                                            aTACGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAt       <  2:927804/151‑1 (MQ=255)                                                                                                                                               cGCTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGCCGCTGGCGTTGGGGATGGCCTACATTCTGCCCGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAttt     <  2:534789/150‑1 (MQ=255)                                                                                                                                                 cTAAGCCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATtttgt  >  1:93410/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGT  >  NC_000913/631031‑631325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑