Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,053,549	A→G	L877P (CTA→CCA)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,053,549	0	A	G	100.0%	223.2 / NA	61	L877P (CTA→CCA)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

TATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTAAATAATACCAG  >  NC_000913/1053401‑1053694                                                                                                                                                     |                                                                                                                                                  tATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGgc                                                                                                                                                 >  2:926512/1‑151 (MQ=255)   ttCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGgctg                                                                                                                                               >  2:436923/1‑151 (MQ=255)       aGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGTCTGCTGCTCCAGTTGTGCGCATGgcgggctg                                                                                                                                           <  1:691831/151‑1 (MQ=255)        gttgttCTTAACTGGCTGATATTGATGCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGctggctgg                                                                                                                                          <  2:999455/151‑1 (MQ=255)          tgttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCg                                                                                                                                        >  2:286822/1‑151 (MQ=255)           gttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCAtggctggctggca                                                                                                                                        <  2:54998/150‑2 (MQ=255)                tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATg                                                                                                                                   <  1:983006/150‑1 (MQ=255)                  aCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCAt                                                                                                                                <  2:185766/151‑1 (MQ=255)                     ggCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTc                                                                                                                              <  1:393689/150‑1 (MQ=255)                      gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGCAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTccc                                                                                                                            >  2:184802/1‑151 (MQ=255)                        tGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTccc                                                                                                                            >  1:944013/1‑149 (MQ=255)                         gATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCa                                                                                                                           <  2:719506/149‑1 (MQ=255)                          atatTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGAc                                                                                                                        >  1:283151/1‑151 (MQ=255)                            atTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACt                                                                                                                       <  1:551276/150‑1 (MQ=255)                              tGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCg                                                                                                                     <  1:122861/150‑1 (MQ=255)                               gATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAg                                                                                                                   <  1:929968/151‑1 (MQ=255)                                aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAg                                                                                                                   >  1:835385/1‑150 (MQ=255)                                aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGc                                                                                                                  >  2:159152/1‑151 (MQ=255)                                     aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTg                                                                                                             >  1:689309/1‑151 (MQ=255)                                      aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGc                                                                                                            >  2:60224/1‑151 (MQ=255)                                        cgcgTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCt                                                                                                           <  2:13215/150‑1 (MQ=255)                                         gcgTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCttt                                                                                                         >  1:515660/1‑151 (MQ=255)                                             tCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCttttt                                                                                                       >  2:937860/1‑149 (MQ=255)                                              cAGGTCTTTCTTATGCAACCATGCTTCCAGCGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGc                                                                                                    >  1:317799/1‑151 (MQ=255)                                              cAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAg                                                                                                     <  1:937909/150‑1 (MQ=255)                                                    tttcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgc                                                                                              >  1:83186/1‑151 (MQ=255)                                                     ttcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGAt                                                                                                <  1:770788/148‑1 (MQ=255)                                                     ttcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcg                                                                                             >  2:706730/1‑151 (MQ=255)                                                     ttcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                                                                                               <  2:396720/149‑1 (MQ=255)                                                      tcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcgc                                                                                            >  2:542150/1‑151 (MQ=255)                                                      tcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcg                                                                                             <  1:217013/150‑1 (MQ=255)                                                         tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                                                                                         >  2:109049/1‑151 (MQ=255)                                                            gCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc                                                                                       <  2:773966/150‑1 (MQ=255)                                                             cAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGc                                                                                     <  1:710191/151‑1 (MQ=255)                                                                  aTGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAt                                                                                <  1:622533/151‑1 (MQ=255)                                                                          agagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGc                                                                        >  2:579184/1‑151 (MQ=255)                                                                          agagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGc                                                                        >  2:369980/1‑151 (MQ=255)                                                                             gCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTAt                                                                     >  1:436300/1‑151 (MQ=255)                                                                             gCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTAt                                                                     >  1:6580/1‑151 (MQ=255)                                                                              cGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATc                                                                    >  1:109873/1‑151 (MQ=255)                                                                                gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCt                                                                  <  2:5191/151‑1 (MQ=255)                                                                                 cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTg                                                                 <  2:906930/151‑1 (MQ=255)                                                                                 cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTg                                                                 >  2:21453/1‑151 (MQ=255)                                                                                  aaCACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGa                                                                <  2:99536/151‑1 (MQ=255)                                                                                     acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTg                                                             <  2:492186/151‑1 (MQ=255)                                                                                      cTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTgg                                                            >  1:241386/1‑151 (MQ=255)                                                                                        gCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGgc                                                           <  1:666396/150‑1 (MQ=255)                                                                                          gtgtAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGcgcg                                                        >  2:686033/1‑151 (MQ=255)                                                                                          gtgtAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGcgcg                                                        <  1:35501/151‑1 (MQ=255)                                                                                          gtgtAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGcgcg                                                        <  1:905033/151‑1 (MQ=255)                                                                                                 ttcttcGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATg                                                 >  1:457304/1‑151 (MQ=255)                                                                                                 ttcttcGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATg                                                 >  1:272985/1‑151 (MQ=255)                                                                                                   cttcGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTc                                               >  2:760269/1‑151 (MQ=255)                                                                                                         gtgGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGAttt                                          <  2:898761/150‑1 (MQ=255)                                                                                                             aaGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCg                                     >  2:319336/1‑151 (MQ=255)                                                                                                               ggggggCAGATAATGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCgag                                   <  2:251188/151‑1 (MQ=255)                                                                                                                            tGGCTGCTGCTCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGc                       >  2:517869/1‑150 (MQ=255)                                                                                                                              gctgctgctCCAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCAt                     >  1:362508/1‑150 (MQ=255)                                                                                                                                       ccAGTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTa             <  1:106415/149‑1 (MQ=255)                                                                                                                                          gTTGTGCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGTCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTaaa           >  2:325185/1‑148 (MQ=255)                                                                                                                                              tgCGCATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTAAATAATAcc    >  1:94539/1‑151 (MQ=255)                                                                                                                                                cgcATGGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTAAATAATACCAg  <  1:543950/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGGCAATGTCGATTTCATCGAGAAGCGGCAGGGCATGTTGTGTAAATAATACCAG  >  NC_000913/1053401‑1053694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑