Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,140,385	G→A	R118H (CGT→CAT)	flgL →	flagellar hook‑filament junction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,140,385	0	G	A	100.0%	254.6 / NA	65	R118H (CGT→CAT)	flgL	flagellar hook‑filament junction protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

CCACTCAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCGTGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCT  >  NC_000913/1140238‑1140530                                                                                                                                                    |                                                                                                                                                  ccACTCAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGa                                                                                                                                                <  1:792743/151‑1 (MQ=255)     tCAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGAcc                                                                                                                                              >  1:924663/1‑149 (MQ=255)      cAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGt                                                                                                                                           >  2:238982/1‑151 (MQ=255)      cAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGAGCATCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGt                                                                                                                                           >  2:478439/1‑151 (MQ=255)         aaaGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGc                                                                                                                                        <  2:729735/151‑1 (MQ=255)                 aCTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTg                                                                                                                                >  1:891610/1‑151 (MQ=255)                              gtACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACt                                                                                                                    <  1:10032/150‑1 (MQ=255)                              gtACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACt                                                                                                                    <  2:920454/150‑1 (MQ=255)                                 cTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACg                                                                                                                >  2:468954/1‑151 (MQ=255)                                             accacTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCt                                                                                                    <  1:537087/151‑1 (MQ=255)                                             accacTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCt                                                                                                    >  2:545010/1‑151 (MQ=255)                                               cacTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTAc                                                                                                  <  1:818961/151‑1 (MQ=255)                                               cacTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTAc                                                                                                  >  2:391945/1‑151 (MQ=255)                                                 cTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACAt                                                                                                >  1:463606/1‑151 (MQ=255)                                                   gCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACAttt                                                                                              >  1:358176/1‑151 (MQ=255)                                                       tCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGc                                                                                          >  1:881214/1‑151 (MQ=255)                                                         cAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCg                                                                                        <  1:7500/151‑1 (MQ=255)                                                           taaTGCTCAGTAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGt                                                                                      >  2:929982/2‑151 (MQ=255)                                                             aTGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTta                                                                                    <  1:862662/151‑1 (MQ=255)                                                               gCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTtata                                                                                  <  2:206471/151‑1 (MQ=255)                                                                 tCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATaa                                                                                 >  1:110503/1‑150 (MQ=255)                                                                 tCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATaa                                                                                 >  2:832659/1‑150 (MQ=255)                                                                  cAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATaaaa                                                                               <  2:140027/151‑1 (MQ=255)                                                                  cAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATaa                                                                                 <  1:631044/149‑1 (MQ=255)                                                                     gAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACag                                                                            <  2:349409/151‑1 (MQ=255)                                                                      aaaaaaTTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAAc                                                                              >  1:375344/1‑148 (MQ=255)                                                                      aaaaaaTTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACaga                                                                           <  2:51195/151‑1 (MQ=255)                                                                        aaaaTTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAgaga                                                                         <  1:941256/151‑1 (MQ=255)                                                                          aaTTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACt                                                                       >  1:734605/1‑151 (MQ=255)                                                                            ttGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTgc                                                                     >  2:405826/1‑151 (MQ=255)                                                                             tGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTgcg                                                                    >  1:267209/1‑151 (MQ=255)                                                                              gTCTACGCCAGCAATGGCACATTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTgcg                                                                    >  2:940081/1‑150 (MQ=255)                                                                                 tACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGt                                                                >  1:203065/1‑151 (MQ=255)                                                                                 tACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGt                                                                >  1:45742/1‑151 (MQ=255)                                                                                   cGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGcc                                                                  <  1:937132/147‑1 (MQ=255)                                                                                    gCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTa                                                             <  1:119021/151‑1 (MQ=255)                                                                                    gCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTa                                                             <  1:434635/151‑1 (MQ=255)                                                                                       aGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCg                                                          <  1:71001/151‑1 (MQ=255)                                                                                                  cTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAggg                                               >  2:507933/1‑151 (MQ=255)                                                                                                  cTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAggg                                               >  1:17676/1‑151 (MQ=255)                                                                                                    tGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGa                                              <  2:666319/150‑1 (MQ=255)                                                                                                     gAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGaa                                             >  2:88368/1‑150 (MQ=255)                                                                                                      aGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAAt                                           <  1:947014/151‑1 (MQ=255)                                                                                                        tGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATAc                                         >  1:372584/1‑151 (MQ=255)                                                                                                         gACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACg                                        >  2:934667/1‑151 (MQ=255)                                                                                                           cGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTc                                      >  2:703037/1‑151 (MQ=255)                                                                                                           cGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTc                                      <  2:974145/151‑1 (MQ=255)                                                                                                            gATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCg                                     >  2:29293/1‑151 (MQ=255)                                                                                                                  cGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTgg                                 >  2:369539/1‑149 (MQ=255)                                                                                                                  cGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAg                               <  2:250035/151‑1 (MQ=255)                                                                                                                  cGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAg                               <  1:641912/151‑1 (MQ=255)                                                                                                                     gCCTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGc                              >  1:1019641/1‑149 (MQ=255)                                                                                                                      ccTCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGa                           <  1:22366/151‑1 (MQ=255)                                                                                                                        tagCTGGCTACGGATATTCCGGGGCTTCATGACGAGTTGCTGAATCTGGCAAACACCACTGACGGTAACTGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGTAGAGAAAGGGAAATACGTCGGTGGAGCAGaa                          >  2:327563/3‑150 (MQ=255)                                                                                                                        tCGCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGaa                          >  1:939962/1‑150 (MQ=255)                                                                                                                          gCTGGCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGt                       >  1:853756/1‑151 (MQ=255)                                                                                                                             ggCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTa                      <  1:382682/149‑1 (MQ=255)                                                                                                                             ggCTACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTAtt                    <  2:465784/151‑1 (MQ=255)                                                                                                                                tACGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTaa                  >  1:42937/1‑150 (MQ=255)                                                                                                                                 aCGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTaa                  <  1:908255/149‑1 (MQ=255)                                                                                                                                 aCGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTaa                  <  1:36527/149‑1 (MQ=255)                                                                                                                                 aCGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAaac                >  1:126589/1‑151 (MQ=255)                                                                                                                                  cGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTaa                  <  2:856197/148‑1 (MQ=255)                                                                                                                                  cGGATATTCAGGGGCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAaac                >  1:328464/1‑150 (MQ=255)                                                                                                                                               gCTTCATGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCt  <  1:242520/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCACTCAAAAAGTGTCACTGGAAGAGAGTGTACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAATTGTCTACGCCAGCAATGGCACCTTGAGTGACGATGACCGGGCCTCGCTGGCTACGGATATTCAGGGGCTTCGTGACCAGTTGCTGAATCTGGCAAACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCT  >  NC_000913/1140238‑1140530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑