Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 209,483 C→T A288V (GCG→GTG)  accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,4830CT98.5% 244.0 / NA 66A288V (GCG→GTG) accAacetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/32);  minor base A (1/0);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGC  >  NC_000913/209337‑209629
                                                                                                                                                  |                                                                                                                                                  
gagCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCa                                                                                                                                                 >  1:726137/1‑150 (MQ=255)
          aaaGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCgg                                                                                                                                      <  2:43346/151‑1 (MQ=255)
            aGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGAt                                                                                                                                    <  2:1026899/151‑1 (MQ=255)
             gcgcCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATc                                                                                                                                   >  2:228899/1‑151 (MQ=255)
              cgccgcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCt                                                                                                                                  <  1:361626/151‑1 (MQ=255)
                  gcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGcc                                                                                                                              >  1:113773/1‑151 (MQ=255)
                  gcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGcc                                                                                                                              <  2:123217/151‑1 (MQ=255)
                  gcTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGcc                                                                                                                              >  1:967796/1‑151 (MQ=255)
                    tGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCg                                                                                                                             >  2:1014427/1‑150 (MQ=255)
                    tGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCg                                                                                                                             >  2:406815/1‑150 (MQ=255)
                     ggcggcTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCg                                                                                                                             <  1:898261/149‑1 (MQ=255)
                     ggcggcTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGAt                                                                                                                           >  2:227625/1‑151 (MQ=255)
                           tGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGAc                                                                                                                     >  2:313110/1‑151 (MQ=255)
                                  aTGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTa                                                                                                               >  1:443854/1‑150 (MQ=255)
                                  aTGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTa                                                                                                               >  1:672715/1‑150 (MQ=255)
                                   tGGGTGTCATTGCGCTGCGTCTGAAAGAACTGAAACTGACCGAGTCCATAATCCCGGAACCGCTGGGTGTTGCTCACCGTAACCCGGAAGAGATGTCGGCATCGTTGAAAGTGCGACGGCTGGCGGATCTGGCCGATCTCGACGGGTTa                                                                                                               <  2:608784/149‑1 (MQ=255)
                                           aTTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGa                                                                                                       >  1:784733/1‑149 (MQ=255)
                                             tGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGa                                                                                                    <  2:109465/150‑1 (MQ=255)
                                                 ccGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGAttt                                                                                                 <  1:96262/149‑1 (MQ=255)
                                                              aactgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGtt                                                                                   >  1:545997/1‑150 (MQ=255)
                                                              aactgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTa                                                                                  <  1:679545/151‑1 (MQ=255)
                                                              aaatgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGAGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGtt                                                                                   >  2:931147/4‑150 (MQ=255)
                                                               actgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTAt                                                                                 <  2:213033/151‑1 (MQ=255)
                                                               actgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTAt                                                                                 <  2:518155/151‑1 (MQ=255)
                                                               actgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTAt                                                                                 >  2:260547/1‑151 (MQ=255)
                                                                 tgaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                                >  1:982092/1‑150 (MQ=255)
                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTAta                                                                                <  1:571199/149‑2 (MQ=255)
                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                                <  2:1059587/149‑1 (MQ=255)
                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATc                                                                                >  1:399218/1‑149 (MQ=255)
                                                                  gaaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCa                                                                               <  1:813360/150‑1 (MQ=255)
                                                                   aaactgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAgc                                                                             >  2:964542/1‑151 (MQ=255)
                                                                     actgaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAgcgc                                                                           >  1:787361/1‑151 (MQ=255)
                                                                        gaTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCAGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCt                                                                         <  2:498109/150‑1 (MQ=255)
                                                                         aTCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtg                                                                        >  1:919129/1‑150 (MQ=255)
                                                                           cGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtgatg                                                                     >  1:78685/1‑151 (MQ=255)
                                                                               tCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCt                                                                 >  1:774304/1‑151 (MQ=255)
                                                                                 catcatCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGc                                                                  <  1:569415/148‑1 (MQ=255)
                                                                                   tcatcCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACgg                                                             >  2:423923/1‑151 (MQ=255)
                                                                                    catcCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACgg                                                             <  2:686912/150‑1 (MQ=255)
                                                                                       cccGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTAc                                                         <  1:35905/151‑1 (MQ=255)
                                                                                          ggAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTAcgcg                                                      <  2:73334/151‑1 (MQ=255)
                                                                                           gAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtgatga                                                                    >  2:925877/1‑136 (MQ=255)
                                                                                           gAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCtgatga                                                                    <  1:925924/136‑1 (MQ=255)
                                                                                             aCCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTa                                                    >  1:679395/1‑150 (MQ=255)
                                                                                             aCCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTa                                                    >  2:502508/1‑150 (MQ=255)
                                                                                                aCTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTc                                                >  2:272286/1‑151 (MQ=255)
                                                                                                 cTGGGTGGTGCTCACCGTAGCCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTc                                                <  2:484033/150‑1 (MQ=255)
                                                                                                  tGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGc                                              >  1:569/1‑151 (MQ=255)
                                                                                                   gggTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCa                                             <  1:215524/151‑1 (MQ=255)
                                                                                                     gtggtgCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCaaa                                           <  1:298554/151‑1 (MQ=255)
                                                                                                          gCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTc                                      <  1:853176/151‑1 (MQ=255)
                                                                                                              aCCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGa                                   >  2:220996/1‑150 (MQ=255)
                                                                                                                 gTAACCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGaaaa                                >  2:971822/1‑150 (MQ=255)
                                                                                                                    aCCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGATCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAggg                            <  1:40595/151‑1 (MQ=255)
                                                                                                                    aCCCGGAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAggg                            <  2:569/151‑1 (MQ=255)
                                                                                                                        ggAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCAc                        >  1:219911/1‑151 (MQ=255)
                                                                                                                         gAAGCGATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCAc                        >  2:45172/1‑150 (MQ=255)
                                                                                                                              gATGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGt                  <  2:845644/151‑1 (MQ=255)
                                                                                                                               aTGGCGGCATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTg                 <  1:625367/151‑1 (MQ=255)
                                                                                                                                      cATCGTTGAAAGTGCAACTGCTGGCGGTTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGCGCTACGGTTACGCGTAATTCCCAAAAGTTCTGAAACAGGGTCCCTTCGGTGGCCCttt          <  2:233745/151‑1 (MQ=255)
                                                                                                                                      cATCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCttt          >  1:167582/1‑151 (MQ=255)
                                                                                                                                       aTCGTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttt         <  2:789057/151‑1 (MQ=255)
                                                                                                                                          gTTGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTa      <  1:767376/151‑1 (MQ=255)
                                                                                                                                           ttGAAAGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTAt     >  2:525699/1‑151 (MQ=255)
                                                                                                                                              aaaGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGc  <  1:275258/151‑1 (MQ=255)
                                                                                                                                              aaaGTGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGc  >  1:115540/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAGCGCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAACTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAAGCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGC  >  NC_000913/209337‑209629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑