Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,259,033 A→G K81K (AAA→AAG)  ychH → DUF2583 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,259,0330AG92.7% 166.0 / ‑4.1 55K81K (AAA→AAG) ychHDUF2583 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (4/0);  new base G (14/37);  total (18/37)
Fisher's exact test for biased strand distribution p-value = 8.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCC  >  NC_000913/1258886‑1259175
                                                                                                                                                   |                                                                                                                                              
gTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGt                                                                                                                                             <  2:795714/151‑1 (MQ=255)
  tACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGtt                                                                                                                                            <  1:518332/150‑1 (MQ=255)
       caGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGGCCGTTACTGGGGGGGTCGCCACTATGACAAACGTTgccccc                                                                                                                                      >  2:338739/1‑151 (MQ=255)
       caGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCACTATGACAAGCGTTgccgcc                                                                                                                                      >  1:245002/1‑151 (MQ=255)
         gTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGt                                                                                                                                    <  2:874628/151‑1 (MQ=255)
                atGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGAt                                                                                                                              <  1:907787/150‑1 (MQ=255)
                  gCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAAt                                                                                                                           <  2:830435/151‑1 (MQ=255)
                  gCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAAt                                                                                                                           <  2:286110/151‑1 (MQ=255)
                    cccAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCACTATGACAAGCGTTGCCGCCGGAGCGATAAt                                                                                                                           >  1:823397/1‑149 (MQ=255)
                    cccAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGCGTGCGACCGTTACTGGTGGGTTCGCCACTATTACAAGCGTTTCCGCCGCAGCGGTAATc                                                                                                                          >  1:576742/1‑150 (MQ=255)
                       aGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGcca                                                                                                                      <  2:640076/151‑2 (MQ=255)
                          aTTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTc                                                                                                                    <  1:946420/150‑1 (MQ=255)
                             tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATa                                                                                                                 <  2:91173/150‑1 (MQ=255)
                              cGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAg                                                                                                                <  1:1053967/150‑1 (MQ=255)
                               gCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCt                                                                                                              <  1:331826/151‑1 (MQ=255)
                                   aTGGTGCAGCGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACa                                                                                                          <  1:328013/151‑1 (MQ=255)
                                     ggTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACaa                                                                                                         <  1:223986/150‑1 (MQ=255)
                                     ggTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACaa                                                                                                         <  1:148733/150‑1 (MQ=255)
                                     ggTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACaa                                                                                                         <  2:930049/150‑1 (MQ=255)
                                       tGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAaat                                                                                                       >  1:220745/1‑150 (MQ=255)
                                        gCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCTTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAaataa                                                                                                     <  1:167145/151‑1 (MQ=255)
                                         cAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAaataa                                                                                                     <  1:640788/150‑1 (MQ=255)
                                          aGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATg                                                                                                   <  2:920412/151‑1 (MQ=255)
                                                aaGTATTTTCGTCGGTGCCATTCTCTTGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCTTTACTGGTGGTTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGtt                                                                                              <  2:596990/150‑1 (MQ=255)
                                                 aGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTg                                                                                            <  2:929917/151‑1 (MQ=255)
                                                    aTTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTg                                                                                         <  2:544745/151‑1 (MQ=255)
                                                     ttttCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGGGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTTCTg                                                                                         >  2:392377/1‑150 (MQ=255)
                                                                gCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGGGGGCATGAAAAGGTGTGCGACCGGTACTGGGGGGGTCGCCCCTATGACAAGCGTTGCCGCCGTAGCGATAATCGGCGTCATAGGTAACAAATAATGCAGGTTGCTGAACAGTCAAttt                                                                             >  2:1055146/1‑151 (MQ=255)
                                                                gCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTTGGGGGGTCGCCACTATGACAAACGTTTCCGCCGTAACGATAATCGCCGTCATAACTAACAAATAATGCAGTTTTCTGAACAGTCAGgtt                                                                             >  1:1062907/1‑151 (MQ=255)
                                                                  cATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTg                                                                            >  1:769574/1‑150 (MQ=255)
                                                                   aTTCTCTGGCTGGCGGGTGCCCGGGTTGGGGGGCATGAACAGGTGGGCGACCGTTACTGGAGGGGTCGGCACTAAGCCAAACGTTGCCCCCCGCGCGGCAATCCCCGGCCTAACTAACAAATAATTCAGTTTTTTGACcaagcaggtt                                                                             >  2:885242/1‑140 (MQ=255)
                                                                    ttCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTTCTGACCAGTCAGTTTgcgc                                                                         >  1:753819/1‑151 (MQ=255)
                                                                     tctcTGGCTGGCGGGGGCCCGGGTTGGCGGGCATTAAAAAGTGTGCGACCGTTTCTGGGGGGTTCGCCACTATTACAAACGGTGCCGCCGTAGCGATAAACGCCGTCATAGCTAACAAAAAAAGCAGTTTGCTGACCAGTCAGTTTgcgc                                                                         >  2:947915/1‑150 (MQ=255)
                                                                                  ggTGCCCGTGATGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCACCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAAtct                                                           >  2:628268/1‑151 (MQ=255)
                                                                                     gCCCGTGTTGGCGGGCATGAACAGGTGTGGGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCg                                                         <  2:568543/150‑1 (MQ=255)
                                                                                        cGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACt                                                     <  1:1055201/151‑1 (MQ=255)
                                                                                           gtTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTta                                                   <  1:517671/150‑1 (MQ=255)
                                                                                               gCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAAt                                              <  2:516990/151‑1 (MQ=255)
                                                                                                    cATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGcc                                         <  2:63031/151‑1 (MQ=255)
                                                                                                     aTGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCa                                        <  2:97095/151‑1 (MQ=255)
                                                                                                     aTGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCa                                        <  2:359232/151‑1 (MQ=255)
                                                                                                        aaCAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATcg                                     <  1:1067921/151‑1 (MQ=255)
                                                                                                            ggTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATcgccgc                                 <  1:656033/151‑1 (MQ=255)
                                                                                                             gtgtGCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCcgcg                                <  2:758819/151‑1 (MQ=255)
                                                                                                               gtgCGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCcgcgc                               <  2:125076/150‑1 (MQ=255)
                                                                                                                  cGACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGcgacg                           <  2:1014535/151‑1 (MQ=255)
                                                                                                                   gACCGTTACTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGcgacg                           >  1:873184/1‑150 (MQ=255)
                                                                                                                          aCTGGTGGGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCggg                    >  1:14640/1‑150 (MQ=255)
                                                                                                                            tggtggGTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCCGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATAGCCGCGCGACGATTCGGga                   >  2:583684/1‑149 (MQ=255)
                                                                                                                                gggTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAAc              <  1:299314/150‑1 (MQ=255)
                                                                                                                                gggTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACg             >  2:374233/1‑151 (MQ=255)
                                                                                                                                gggTTCGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACg             >  2:591126/1‑151 (MQ=255)
                                                                                                                                     cGCCACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAg         <  2:996889/150‑1 (MQ=255)
                                                                                                                                        cACTATGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGt     <  1:848761/151‑1 (MQ=255)
                                                                                                                                            aTGACAAGCGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTccc  <  1:513720/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTGACTGGTCAATCTCGTACTTATAAATCCGCCATCGCCGCGCGACGATTCGGGAAGAACGCCAGGCGTCCC  >  NC_000913/1258886‑1259175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑