Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,460,132 | A→G | N360S (AAC→AGC) | paaH → | 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,460,132 | 0 | A | G | 98.2% | 191.9 / NA | 58 | N360S (AAC→AGC) | paaH | 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent |
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (26/30); total (28/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGC > NC_000913/1459986‑1460277 | tGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCg > 1:831014/1‑151 (MQ=255) cGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGa < 2:166862/150‑1 (MQ=255) cGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGAc < 2:182715/151‑1 (MQ=255) gtCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGGGGGGACCATTGCTGCTGCCGCGGTGAGCCCGGACTc > 2:322493/1‑149 (MQ=255) cACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGc < 2:385800/150‑1 (MQ=255) cGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGGGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGa > 2:365094/1‑150 (MQ=255) aaaTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGGGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGAccca < 2:560711/151‑2 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGCGGTGATCATTGCTGTTGCAGCGGGGAACCCGGACTCAGCGagc > 2:558284/1‑145 (MQ=255) gACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGGGGTGACCATTGCCGCTTTAGCGGTGAGCCCGGACTCAGCGACCCGCCAgg > 2:465330/1‑151 (MQ=255) aTGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGAGAGCCCGGACTCAGCGACCCGCAAGGCCAt > 2:764953/1‑151 (MQ=255) tttATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATttat > 2:28297/1‑151 (MQ=255) tttATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATttat > 1:514579/1‑151 (MQ=255) tgattgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATTGATAAAATGGCGGGCAAGGAGGGGACCAATGCCGGTGCCGCGGTTATCCCGGACTCAGCGAACCCCCAGGGCTTTTAATAcc > 1:686091/1‑150 (MQ=255) gattgaGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCt < 1:160526/150‑1 (MQ=255) gattgaGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTg > 1:211632/1‑151 (MQ=255) attgaGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTACTACCTGc < 2:1039014/151‑1 (MQ=255) tgaGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGGGACCATTGCTGCTGCAGCGGGGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCt > 2:991032/1‑147 (MQ=255) agaCACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGGGGAGACCATTGCCGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCa > 2:144551/1‑148 (MQ=255) gaCACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCACGGCCATTTATTACCTGCaa > 1:151348/1‑148 (MQ=255) gaCACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAAca < 2:417388/150‑1 (MQ=255) acacAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAAcagc < 2:310850/151‑1 (MQ=255) gCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCACGGTGGTGCCCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCaa < 2:47068/151‑1 (MQ=255) gCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCaa < 1:465355/151‑1 (MQ=255) agaCGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCaaaa > 1:528206/1‑150 (MQ=255) caGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGtgctgc < 2:1000477/151‑1 (MQ=255) gcTGGCAATACTACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTTCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAAtt < 2:812440/151‑1 (MQ=255) ggCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTg < 1:61396/149‑1 (MQ=255) gCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCACGGTGGTGACCAACGCTGCTGCCGCGGTGAGCCCGGACTCAGAGACCCGCAAGGCCATTTATTACCTTCCACAGCCGGGCAAAACAATGCTGCa > 2:1060427/1‑143 (MQ=255) aCGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTAc > 1:334640/1‑151 (MQ=255) tGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAgg > 1:128791/1‑151 (MQ=255) ggCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGa < 2:809736/151‑1 (MQ=255) ccGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGa < 1:606066/151‑1 (MQ=255) ggtAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTgg < 2:796269/151‑1 (MQ=255) ggtAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTgg > 1:553666/1‑151 (MQ=255) gtAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGc < 2:976169/151‑1 (MQ=255) gATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACg < 1:1031770/151‑1 (MQ=255) aTCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGACATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACgg > 1:380729/1‑151 (MQ=255) cGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTa < 2:910404/151‑1 (MQ=255) aTAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGc > 1:1001938/1‑151 (MQ=255) aTAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGc < 1:461975/151‑1 (MQ=255) tAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGc > 2:921753/1‑150 (MQ=255) aaaTGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAAt > 2:449100/1‑151 (MQ=255) gCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCaa < 1:584165/151‑1 (MQ=255) aaGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATg < 2:908465/151‑1 (MQ=255) aaGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATg > 1:6845/1‑151 (MQ=255) aGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGa < 1:477336/151‑1 (MQ=255) aGGTGGTGACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGa < 1:548307/151‑1 (MQ=255) ggtgACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGcc < 2:395445/151‑1 (MQ=255) gtgACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGccc > 2:409348/1‑151 (MQ=255) tgACCATTGCTGCTGCAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCt > 2:441633/1‑151 (MQ=255) tgctgcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCtt > 2:903235/1‑151 (MQ=255) gctgcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCtt < 1:914939/150‑1 (MQ=255) ctgcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTc > 2:865493/1‑150 (MQ=255) gcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCaaa < 1:471138/151‑1 (MQ=255) gcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCa < 1:144553/149‑1 (MQ=255) gcAGCGGTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCa < 1:77207/149‑1 (MQ=255) ggTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGc > 1:698563/1‑151 (MQ=255) ggTGAGCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGc < 1:956683/151‑1 (MQ=255) | TGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGC > NC_000913/1459986‑1460277 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |